Search results for "adem"

Dual targeting of higher-order DNA structures by azacryptands induces DNA junction-mediated DNA damage in cancer cells

2021

Abstract DNA is intrinsically dynamic and folds transiently into alternative higher-order structures such as G-quadruplexes (G4s) and three-way DNA junctions (TWJs). G4s and TWJs can be stabilised by small molecules (ligands) that have high chemotherapeutic potential, either as standalone DNA damaging agents or combined in synthetic lethality strategies. While previous approaches have claimed to use ligands that specifically target either G4s or TWJs, we report here on a new approach in which ligands targeting both TWJs and G4s in vitro demonstrate cellular effects distinct from that of G4 ligands, and attributable to TWJ targeting. The DNA binding modes of these new, dual TWJ-/G4-ligands w…

gNOMO: a multi-omics pipeline for integrated host and microbiome analysis of non-model organisms

2020

The study of bacterial symbioses has grown exponentially in the recent past. However, existing bioinformatic workflows of microbiome data analysis do commonly not integrate multiple meta-omics levels and are mainly geared toward human microbiomes. Microbiota are better understood when analyzed in their biological context; that is together with their host or environment. Nevertheless, this is a limitation when studying non-model organisms mainly due to the lack of well-annotated sequence references. Here, we present gNOMO, a bioinformatic pipeline that is specifically designed to process and analyze non-model organism samples of up to three meta-omics levels: metagenomics, metatranscriptomic…

Between Interactions and Aggregates: The PolyQ Balance

2021

Abstract Polyglutamine regions (polyQ) are highly abundant consecutive runs of glutamine residues. They have been generally studied in relation to the so-called polyQ-associated diseases, characterized by protein aggregation caused by the expansion of the polyglutamine tract via a CAG-slippage mechanism. However, more than 4800 human proteins contain a polyQ, and only 9 of these regions are known to be associated with disease. Computational sequence studies and experimental structure determinations are completing a more interesting picture in which polyQ emerge as a motif for modulation of protein-protein interactions. But long polyQ regions may lead to an excess of interactions, and produc…

Simulating the impact of non-pharmaceutical interventions limiting transmission in COVID-19 epidemics using a membrane computing model

2021

Epidemics caused by microbial organisms are part of the natural phenomena of increasing biological complexity. The heterogeneity and constant variability of hosts, in terms of age, immunological status, family structure, lifestyle, work activities, social and leisure habits, daily division of time and other demographic characteristics make it extremely difficult to predict the evolution of epidemics. Such prediction is, however, critical for implementing intervention measures in due time and with appropriate intensity. General conclusions should be precluded, given that local parameters dominate the flow of local epidemics. Membrane computing models allows us to reproduce the objects (virus…

Dianthus superbus as a critically endangered species in Latvia: evaluation of its growth conditions and conservation possibilities

2021

Abstract Dianthus superbus is one of the most endangered species in Latvia and is on the verge of local extinction. Therefore, the aim of this study was to inventory previously identified populations of D. superbus in Latvia and to develop activities to conserve this species in accordance with the results obtained in situ. Expeditions on 18 previously documented locations, according to the data of Nature Conservation Agency, revealed only three existing D. superbus localities in Latvia with a significant number of specimens located in the Latgale region near Silenieki. In 2020, for the first time, two more new D. superbus localities were found in the vicinity of these three approved locatio…

Molecular mechanisms of endomembrane trafficking in plants

2021

Abstract Endomembrane trafficking is essential for all eukaryotic cells. The best-characterized membrane trafficking organelles include the endoplasmic reticulum (ER), Golgi apparatus, early and recycling endosomes, multivesicular body, or late endosome, lysosome/vacuole, and plasma membrane. Although historically plants have given rise to cell biology, our understanding of membrane trafficking has mainly been shaped by the much more studied mammalian and yeast models. Whereas organelles and major protein families that regulate endomembrane trafficking are largely conserved across all eukaryotes, exciting variations are emerging from advances in plant cell biology research. In this review, …

Two New Alternatives to the Conventional Arm-in-Cage Test for Assessing Topical Repellents

2021

Abstract European guidelines for testing attractant and repellent efficacy (i.e., Product type 19 [PT19]) have been in revision since 2017. A key topic of discussion is the current approach to evaluating topical repellents. The European Chemical Agency has stated field testing should be avoided because of mosquito-borne disease risks. However, the most common laboratory method, the arm-in-cage (AIC) test, may limit the reliable extrapolation of lab results to field conditions. This study’s main goal was to assess alternative laboratory methods for evaluating topical mosquito repellents that use mosquito landing rates more representative of those in the field. The study took place at three E…

The neuroinflammation collection: a vision for expanding neuro-immune crosstalk in Brain

2021

La herencia clásica palladiana en dos proyectos catedralicios valencianos: la renovación de la Puerta de los Apóstoles

2005

El presente artículo pretende analizar los proyectos de renovación de la Puerta de los Apóstoles, en la catedral de Valencia. Estos proyectos son los de Vicente Marzo y José García, realizados ambos en el año 1796. A partir de ellos se buscarán sus influencias que remiten, especialmente, a I Quattro Libri dell'Architettura de Palladio. (A)

El arquitecto Lorenzo Martínez

1989

LORENZO MARTINEZ ES UNO DE LOS PRINCIPALES ARTIFICES CONSTRUCTORES DE LA VALENCIA DE LA SEGUNDA MITAD DEL SIGLO XVIII. CONTEMPORANEO DE ANTONIO GILABERT, CON EL QUE INTERVINO EN LA REMODELACION CLASICISTA DE LA CATEDRAL DE VALENCIA --Y EN BUENA MEDIDA TAMBIEN DE FELIPE RUBIO Y MULET, VICENTE GASCO, JUAN BAUTISTA MINGUEZ Y MUCHOS OTROS-- FUE MAESTRO MAYOR DE OBRAS DE LA CIUDAD Y ARQUITECTO ACADEMICO.-- LA EPOCA QUE LE TOCO VIVIR, DE HONDA TRASCENDENCIA, SUPONE EL TRANSITO HACIA LA MODERNIDAD EN LA ARQUITECTURA VALENTINA CON EL RELEVO EN EL DOMINIO DE SU GESTION ENTRE EL GREMIO DE ALBAÑILES, QUE PERECE VIRTUALMENTE EN 1787, Y LA SECCION DE ARQUITECTURA DE LA REAL ACADEMIA DE BELLAS ARTES DE S…