Search results for "aged"

showing 10 items of 17367 documents

Unexpected subcellular distribution of a specific isoform of the Coxsackie and adenovirus receptor, CAR-SIV, in human pancreatic beta cells

2018

Aims/hypothesis: The Coxsackie and adenovirus receptor (CAR) is a transmembrane cell-adhesion protein that serves as an entry receptor for enteroviruses and may be essential for their ability to infect cells. Since enteroviral infection of beta cells has been implicated as a factor that could contribute to the development of type 1 diabetes, it is often assumed that CAR is displayed on the surface of human beta cells. However, CAR exists as multiple isoforms and it is not known whether all isoforms subserve similar physiological functions. In the present study, we have determined the profile of CAR isoforms present in human beta cells and monitored the subcellular localisation of the princi…

0301 basic medicineMaleviruksetEndocrinology Diabetes and MetabolismInsulin-Secreting CellsProtein IsoformsReceptorChildProinsulinEnterovirusMicroscopy ConfocalChemistryNuclear ProteinsImmunogold labellingMiddle AgedFlow CytometryImmunohistochemistryTransmembrane protein3. Good healthCell biologyEndocrinologieenteroviruksetMédecine interneProtein interacting with C-kinase 1 (PICK1)medicine.anatomical_structureChild PreschoolCoxsackievirus BFemalePancreasPICK1Gene isoformBeta cells; Coxsackie and adenovirus receptor; Coxsackievirus B; Enterovirus; Insulin granule; Pancreas; Protein interacting with C-kinase 1 (PICK1)AdultCoxsackie and Adenovirus Receptor-Like Membrane ProteinAdolescentImmunoprecipitationBlotting WesterninsuliiniArticle03 medical and health sciencesYoung AdultMétabolismeInternal MedicinemedicineHumansImmunoprecipitationPancreasCoxsackie and adenovirus receptorInsulin granuleDiabétologieBeta cellshaima030104 developmental biologyDiabetes Mellitus Type 1Carrier ProteinsDiabetologia
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Inference of SARS-CoV-2 spike-binding neutralizing antibody titers in sera from hospitalized COVID-19 patients by using commercial enzyme and chemilu…

2021

medRxiv: https://doi.org/10.1101/2020.09.07.20188151

0301 basic medicineMalevirusesAntibodies ViralImmunoglobulin Glaw.invention0302 clinical medicineMedical microbiologylawMedicine030212 general & internal medicineNeutralizing antibodyImmunoassaychemistry.chemical_classificationmedicine.diagnostic_testbiologyAntibody titerGeneral MedicineMiddle AgedHospitalizationTiterInfectious DiseasesSpike Glycoprotein CoronavirusOriginal ArticleFemaleAntibodyMicrobiology (medical)Adultmedicine.medical_specialtySevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)030106 microbiologyNeutralizing antibodiesSensitivity and SpecificityVirusCOVID-19 Serological Testing03 medical and health sciencesEnzyme-linked immunosorbent assayNeutralization TestsHumansAgedChemiluminescenceSARS-CoV-2business.industryfungiCOVID-19Antibodies NeutralizingVirologyChemiluminescent immunoassaysKineticsEnzymechemistryImmunoassayImmunoglobulin Gbiology.proteinbusiness
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Epidemiologic and Clinical Characteristics of Coronavirus and Bocavirus Respiratory Infections after Allogeneic Stem Cell Transplantation: A Prospect…

2018

Highlights • Human coronavirus are common after allogeneic stem cell transplantation and can progress to LRTDs, and in some cases this leads to hospitalization and requires supportive care. • Human bocavirus are quite rare after allogeneic stem cell transplantation and are commonly detected in conjunction with other viral co-pathogens.

0301 basic medicineMalevirusesBocavirus Community-acquired respiratory virusmedicine.disease_cause0302 clinical medicineHuman bocavirus030212 general & internal medicineProspective StudiesRespiratory Tract InfectionsCoronavirusRespiratory tract infectionsbiologyHuman bocavirusHematopoietic Stem Cell Transplantationvirus diseasesHematologyMiddle AgedAllograftsHospitalizationViral pneumoniaRespiratory virusFemaleCoronavirus InfectionsAdultmedicine.medical_specialtyViral pneumonia030106 microbiologyContext (language use)ArticleParvoviridae InfectionsBocavirus03 medical and health sciencesCommunity-acquired respiratory virusInternal medicinemedicineHumansAgedTransplantationbusiness.industrymedicine.diseasebiology.organism_classificationVirologyrespiratory tract diseasesAllogeneic stem cell transplantationTransplantationCoronavirusRespiratory failureRespiratory virus infectionbusinessFollow-Up Studies
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Determinants of the Quality of Warfarin Control after Venous Thromboembolism and Validation of the SAMe-TT2-R2 Score: An Analysis of Hokusai-VTE.

2019

Background Time in therapeutic range (TTR) measures the quality of vitamin K antagonist (VKA) anticoagulation. In patients with atrial fibrillation, the dichotomized SAMe-TT2-R2 score (≥2 vs. < 2 points) can predict if adequate TTR is unlikely to be achieved. Aims We validated the SAMe-TT2-R2 score in patients with venous thromboembolism (VTE) randomized to the warfarin arm of the Hokusai-VTE trial. Patients and Methods A total of 3,874 patients were included in the primary analysis (day 31–180 from randomization). The efficacy and safety outcomes were symptomatic recurrent VTE and major or clinically relevant non-major bleeding. Results The rates of recurrent VTE and bleeding events we…

0301 basic medicineMalevitamin K antagonistEXTERNAL VALIDATIONTime FactorsVitamin KWarfarin/therapeutic use030204 cardiovascular system & hematologyTHERAPYSeverity of Illness Indexlaw.inventionchemistry.chemical_compound0302 clinical medicineRandomized controlled trialEdoxabanlawRecurrenceAtrial FibrillationVitamin K/antagonists & inhibitorsStrokeRISKAtrial fibrillationHematologyVenous ThromboembolismVitamin K antagonistMiddle Agedrisk assessment modelTIMEPREDICTSTreatment OutcomeAnticoagulants/therapeutic useResearch DesignANTICOAGULATION CONTROLFemaleLife Sciences & Biomedicinemedicine.drugHemorrhage/drug therapyAdultmedicine.medical_specialtyRandomizationmedicine.drug_classvenous thromboembolismHemorrhageRisk AssessmentSensitivity and SpecificityEDOXABAN03 medical and health sciencesDouble-Blind MethodVITAMIN-K ANTAGONISTSInternal medicinemedicineNONVALVULAR ATRIAL-FIBRILLATIONORAL ANTICOAGULANTHumansInternational Normalized RatioBlood CoagulationScience & Technologybusiness.industryquality of treatmentWarfarinAnticoagulantsmedicine.diseasewarfarinClinical trial030104 developmental biologyPeripheral Vascular DiseasechemistryBlood Coagulation/drug effectsAtrial Fibrillation/bloodCardiovascular System & CardiologyLinear ModelsWarfarinbusinessVenous Thromboembolism/drug therapyThrombosis and haemostasis
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Dairy product consumption and risk of type 2 diabetes in an elderly Spanish Mediterranean population at high cardiovascular risk.

2015

PURPOSE The possible effects of dairy consumption on diabetes prevention remain controversial. The aim of this study was to investigate the association between the dairy consumption and type 2 diabetes (T2D) risk in an elderly Mediterranean population at high cardiovascular risk. METHODS: We prospectively followed 3,454 non-diabetic individuals from the PREDIMED study. Dairy consumption was assessed at baseline and yearly using food frequency questionnaires and categorized into total, low-fat, whole-fat, and subgroups: milk, yogurt, cheeses, fermented dairy, concentrated full fat, and processed dairy. Hazard ratios (HRs) were calculated using Cox proportional hazards regression models. RESU…

0301 basic medicineMediterranean climateMaleMultivariate statisticsMedicine (miscellaneous)Type 2 diabetesBody Mass IndexCheeseRisk FactorsSurveys and QuestionnairesMedicineFood scienceProspective StudiesDiet Fat-RestrictedAged 80 and overeducation.field_of_studyNutrition and DieteticsMediterranean RegionIncidenceHazard ratioProductes lactis -- Aspectes nutritiusType 2 diabetesMiddle AgedYogurtMilkCardiovascular DiseasesOlder adultsFemaleDietary ProteinsWaist CircumferencePREDIMEDPopulationDairy03 medical and health sciencesEnvironmental healthDiabetes mellitusDietary CarbohydratesAnimalsHumanseducationTriglyceridesAgedProportional Hazards ModelsConsumption (economics)030109 nutrition & dieteticsDiabetis -- Investigacióbusiness.industryCholesterol HDLCholesterol LDLmedicine.diseasePredimedDietary FatsNutrition AssessmentDiabetes Mellitus Type 2Multivariate AnalysisDairy ProductsbusinessEnergy IntakeFollow-Up Studies
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HIV infection results in metabolic alterations in the gut microbiota different from those induced by other diseases.

2016

Imbalances in gut bacteria have been associated with multiple diseases. However, whether there are disease-specific changes in gut microbial metabolism remains unknown. Here, we demonstrate that human immunodeficiency virus (HIV) infection (n=33) changes, at quantifiable levels, the metabolism of gut bacteria. These changes are different than those observed in patients with the auto-immune disease systemic lupus erythaematosus (n=18), and Clostridium difficile-associated diarrhoea (n=6). Using healthy controls as a baseline (n=16), we demonstrate that a trend in the nature and directionality of the metabolic changes exists according to the type of the disease. The impact on the gut microbia…

0301 basic medicineMetabolite030106 microbiologyMicrobial metabolismHIV InfectionsDiseaseBiologyGut floraArticle03 medical and health scienceschemistry.chemical_compoundMetabolic flux analysismedicineMetabolomeHumansskin and connective tissue diseasesMultidisciplinaryBacteriaMiddle Agedmedicine.diseasebiology.organism_classificationMetabolic Flux AnalysisGastrointestinal MicrobiomeGastrointestinal Tract030104 developmental biologychemistrySpainImmunologyMetabolomeDysbiosissense organsDysbiosisFlux (metabolism)Scientific reports
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Identification of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome-associated DNA methylation patterns.

2018

BackgroundMyalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a complex condition involving multiple organ systems and characterized by persistent/relapsing debilitating fatigue, immune dysfunction, neurological problems, and other symptoms not curable for at least 6 months. Disruption of DNA methylation patterns has been tied to various immune and neurological diseases; however, its status in ME/CFS remains uncertain. Our study aimed at identifying changes in the DNA methylation patterns that associate with ME/CFS.MethodsWe extracted genomic DNA from peripheral blood mononuclear cells from 13 ME/CFS study subjects and 12 healthy controls and measured global DNA methylation by EL…

0301 basic medicineMicroarrayMicroarraysPathology and Laboratory MedicineBiochemistryEpigenesis GeneticCohort StudiesMedicine and Health SciencesSmall nucleolar RNAsPromoter Regions GeneticFatigueAntisense RNARegulation of gene expressionMultidisciplinaryDNA methylationFatigue Syndrome ChronicQRMethylationGenomicsMiddle AgedChromatin3. Good healthNucleic acidsBioassays and Physiological AnalysisCpG siteDNA methylationMedicineEpigeneticsFemaleDNA microarrayDNA modificationChromatin modificationResearch ArticleChromosome biologymusculoskeletal diseasesCell biologyScienceBiologyResearch and Analysis Methods03 medical and health sciencesSigns and SymptomsGenomic MedicineDiagnostic MedicineChronic fatigue syndromemedicineGeneticsHumansGene RegulationEpigeneticsNon-coding RNABiology and life sciencesDNAmedicine.diseaseMicroarray Analysis030104 developmental biologyImmunologyRNACpG IslandsGene expressionPLoS ONE
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The diagnosis of chronic endometritis in infertile asymptomatic women: a comparative study of histology, microbial cultures, hysteroscopy, and molecu…

2017

Background Chronic endometritis is a persistent inflammation of the endometrial mucosa caused by bacterial pathogens such as Enterobacteriaceae, Enterococcus, Streptococcus, Staphylococcus, Mycoplasma, and Ureaplasma. Although chronic endometritis can be asymptomatic, it is found in up to 40% of infertile patients and is responsible for repeated implantation failure and recurrent miscarriage. Diagnosis of chronic endometritis is based on hysteroscopy of the uterine cavity, endometrial biopsy with plasma cells being identified histologically, while specific treatment is determined based on microbial culture. However, not all microorganisms implicated are easily or readily culturable needing …

0301 basic medicineMicrobiological cultureBiopsyStaphylococcusChlamydia trachomatismedicine.disease_causeGastroenterologyUreaplasmaEndometriumGonorrhea0302 clinical medicineGardnerella vaginalisPathology MolecularAsymptomatic InfectionsEscherichia coli Infections030219 obstetrics & reproductive medicinebiologymedicine.diagnostic_testObstetrics and GynecologyHigh-Throughput Nucleotide SequencingBacterial InfectionsMiddle AgedStaphylococcal InfectionsGardnerella vaginalisMycoplasma hominisKlebsiella pneumoniaeFemaleEndometritisInfertility FemaleAdultDNA Bacterialmedicine.medical_specialtyPlasma CellsMycoplasma hominisHysteroscopyReal-Time Polymerase Chain ReactionSensitivity and Specificity03 medical and health sciencesYoung AdultMolecular microbiologyInternal medicineCulture TechniquesStreptococcal InfectionsmedicineEscherichia coliHumansMycoplasma InfectionsGram-Positive Bacterial Infectionsbusiness.industryStreptococcusSequence Analysis DNAChlamydia Infectionsbiology.organism_classificationNeisseria gonorrhoeaeKlebsiella Infections030104 developmental biologyChronic DiseasebusinessChronic EndometritisChlamydia trachomatisEnterococcusEndometrial biopsyAmerican journal of obstetrics and gynecology
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The usefulness of NEWS2 at day 7 of hospitalization in predicting COVID-19 evolution and as an early endpoint in therapeutic trials

2021

Highlights • There is a need for reliable tools to predict the evolution of hospitalized patients suffering from COVID-19 • The likelihood of unfavourable evolution for patients hospitalized for COVID-19 with a National • Early Warning Score 2 (NEWS2) below 7 at Day 7 of hospitalization is nearly non-existent • Such a score could thus be used to allow earlier discharge of the patients and as a judgement criterion in therapeutic trials

0301 basic medicineMicrobiology (medical)2019-20 coronavirus outbreakmedicine.medical_specialtyCoronavirus disease 2019 (COVID-19)Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)030106 microbiologyNEWS2Communicable DiseasesArticle03 medical and health sciences0302 clinical medicineMedicineHumans030212 general & internal medicineIntensive care medicineUnfavourable eventAgedbusiness.industrySARS-CoV-2COVID-19Therapeutic trialCoronavirusHospitalizationInfectious DiseasesItalySARS-CoV2businessPredictionThe Journal of Infection
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Validate or falsify: Lessons learned from a microscopy method claimed to be useful for detecting Borrelia and Babesia organisms in human blood.

2016

A modified microscopy protocol (the LM-method) was used to demonstrate what was interpreted as Borrelia spirochetes and later also Babesia sp., in peripheral blood from patients. The method gained much publicity, but was not validated prior to publication, which became the purpose of this study using appropriate scientific methodology, including a control group.Blood from 21 patients previously interpreted as positive for Borrelia and/or Babesia infection by the LM-method and 41 healthy controls without known history of tick bite were collected, blinded and analysed for these pathogens by microscopy in two laboratories by the LM-method and conventional method, respectively, by PCR methods i…

0301 basic medicineMicrobiology (medical)AdultDNA BacterialAdolescent030106 microbiologyBabesiaPolymerase Chain ReactionSensitivity and SpecificityMicrobiology03 medical and health sciencesYoung AdultLyme diseaseBorreliaBabesiosisparasitic diseasesMedicineAnimalsHumansChildAgedLyme DiseaseMicroscopyGeneral Immunology and MicrobiologyHuman bloodbiologybusiness.industryLyme borreliosisBorreliaInfantBabesiosisGeneral MedicineDNA ProtozoanMiddle Agedbiology.organism_classificationmedicine.diseaseVirologyPeripheral blood030104 developmental biologyInfectious DiseasesChild PreschoolBabesiaFemalebusinessInfectious diseases (London, England)
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