Search results for "alignment"
showing 10 items of 627 documents
Sponge Bcl-2 homologous protein (BHP2-GC) confers distinct stress resistance to human HEK-293 cells
2001
It is established that sponges, the phylogenetically oldest still extant phylum of Metazoa, possess key molecules of the apoptotic pathways, that is members from the Bcl-2 family and a pro-apoptotic molecule with death domains. Here we report on transfection studies of human cells with a sponge gene, GCBHP2. Sponge tissue was exposed to heat shock and tributyltin, which caused an upregulation of gene expression of GCBHP2. The cDNA GCBHP2 was introduced into human HEK-293 cells and mouse NIH-3T3 cells; the stable transfection was confirmed by the identification of the transcripts, by Western blotting as well as by immunofluorescence using antibodies raised against the recombinant polypeptide…
Primary Structure of Selected Archaeal Mesophilic and Extremely Thermophilic Outer Surface Layer Proteins
2002
The archaea are recognized as a separate third domain of life together with the bacteria and eucarya. The archaea include the methanogens, extreme halophiles, thermoplasmas, Sulfate reducers and Sulfur metabolizing thermophiles, which thrive in different habitats such as anaerobic niches, salt lakes, and marine hydrothermals systems and continental solfataras. Many of these habitats represent extreme environments in respect to temperature, osmotic pressure and pH-values and remind on the conditions of the early earth. The cell envelope structures were one of the first biochemical characteristics of archaea studied in detail. The most common archaeal cell envelope is composed of a single cry…
Pythium burgundicum sp. nov. isolated from soil samples taken in French vineyards.
2009
During the course of investigation on pythiaceous fungi occurring in the Burgundian vineyards, a new species of Pythium has been isolated. This oomycete is characterized by its nonproliferating and nonsporulating, spherical to cylindrical type of sporangia (hyphal bodies) germinating through germ tubes, smooth-walled oogonia that are supplied with hypogynous, monoclinous or rarely diclinous antheridia, and smooth-walled oospores. The antheridial cells are very prominent and are reminiscent of Pythium bifurcatum, Pythium segnitium and Pythium longandrum described previously by the author. The internal transcribed spacer region of the rRNA of this new species is composed of 883 bases, which i…
Potentialities of a Highway Alignment Optimization Method in an I-BIM Environment
2019
The BIM (Building Information Modeling) approach potential in the civil engineering field opened novel scenarios in the design idea concept, from planning to executive and constructive phases. The related advantages are numerous and not only limited to a real-time interaction among the involved subjects, that can actually operate in an optimized 3D shared environment. Owing to the sharing information philosophy and to the features of various "smart objects" combined in the project, this innovation reduces potential errors and increases the effectiveness of the design solution in terms of both functionality and cost. Despite these advantages, the highway alignment design problem remains very…
Structure and function of the vacuolar Ccc1/VIT1 family of iron transporters and its regulation in fungi
2020
Iron is an essential micronutrient for most living beings since it participates as a redox active cofactor in many biological processes including cellular respiration, lipid biosynthesis, DNA replication and repair, and ribosome biogenesis and recycling. However, when present in excess, iron can participate in Fenton reactions and generate reactive oxygen species that damage cells at the level of proteins, lipids and nucleic acids. Organisms have developed different molecular strategies to protect themselves against the harmful effects of high concentrations of iron. In the case of fungi and plants, detoxification mainly occurs by importing cytosolic iron into the vacuole through the Ccc1/V…
Fine Alignment of Thermographic Images for Robotic Inspection of Parts with Complex Geometries
2022
Increasing the efficiency of the quality control phase in industrial production lines through automation is a rapidly growing trend. In non-destructive testing, active thermography techniques are known for their suitability to allow rapid non-contact and full-field inspections. The robotic manipulation of the thermographic instrumentation enables the possibility of performing inspections of large components with complex geometries by collecting multiple thermographic images from optimal positions. The robotisation of the thermographic inspection is highly desirable to improve assessment speed and repeatability without compromising inspection accuracy. Although integrating a robotic setup fo…
Learning the relevant image features with multiple kernels
2009
This paper proposes to learn the relevant features of remote sensing images for automatic spatio-spectral classification with the automatic optimization of multiple kernels. The method consists of building dedicated kernels for different sets of bands, contextual or textural features. The optimal linear combination of kernels is optimized through gradient descent on the support vector machine (SVM) objective function. Since a na¨ive implementation is computationally demanding, we propose an efficient model selection procedure based on kernel alignment. The result is a weight — learned from the data — for each kernel where both relevant and meaningless image features emerge after training. E…
Plant virus cell-to-cell movement is not dependent on the transmembrane disposition of its movement protein
2009
ABSTRACT The cell-to-cell transport of plant viruses depends on one or more virus-encoded movement proteins (MPs). Some MPs are integral membrane proteins that interact with the membrane of the endoplasmic reticulum, but a detailed understanding of the interaction between MPs and biological membranes has been lacking. The cell-to-cell movement of the Prunus necrotic ringspot virus (PNRSV) is facilitated by a single MP of the 30K superfamily. Here, using a myriad of biochemical and biophysical approaches, we show that the PNRSV MP contains only one hydrophobic region (HR) that interacts with the membrane interface, as opposed to being a transmembrane protein. We also show that a proline resi…
Profile and molecular modeling of 3-(indole-3-yl)-4-(3,4,5-trimethoxyphenyl)-1 H-pyrrole-2,5-dione (1) as a highly selective VEGF-R2/3 inhibitor.
2006
We report on selectivity profiling of 1 in a panel of 20 protein kinases and molecular modeling indicating 1 to be highly active and selective for VEGF-R2/3. Sequence alignment analysis and detailed insights into the ATP binding pockets of targeted protein kinases from the panel result in a unique structural architecture of VEGF-R2 mainly caused by the hydrophobic pocket I, determining the molecular basis for activity and selectivity of 1.
Aligning Two Specifications for Controlling Information Security
2014
Assuring information security is a necessity in modern organizations. Many recommendations for information security management exist, which can be used to define a baseline of information security requirements. ISO/ IEC 27001 prescribes a process for an information security management system, and guidance to implement security controls is provided in ISO/IEC 27002. Finnish National Security Auditing Criteria (KATAKRI) has been developed by the national authorities in Finland as a tool to verify maturity of information security practices. KATAKRI defines both security control objectives and security controls to meet an objective. Here the authors compare and align these two specifications in…