Search results for "alkaline"

Detection of primary DNA damage in Chlamydomonas reinhardtii by means of modified microgel electrophoresis.

1997

The assessment of genotoxic potential in surface water requires test methods, among which are those that detect initial DNA damage in organisms of aquatic biocenosis. The microgel electrophoresis (MGE) "comet assay" was applied to a ubiquitous unicellular green alga (Chlamydomonas reinhardtii) to detect DNA damage caused by genotoxins. For this, the test protocol described by Singh NP et al. [Exp Cell Res 175: 184-191, 1988] was modified. Major modifications were the use of alkaline lysis buffer with ionic detergents and the reduction of preincubation and electrophoresis times. Short-time exposure of Chlamydomonas to the well-known genotoxicants 4-nitroquinoline-1-oxide (4-NQO), N-nitrosodi…

Specific detection of cyclobutane pyrimidine dimers in phytoplankton DNA by a non-radioactive assay based on T4-endonuclease V digestion.

2001

The effect of artificial and natural UV irradiation on DNA in marine phytoplankton Isochrysis galbana monoculture was investigated. The presence of cyclobutane pyrimidine dimers (CPDs) in unlabelled I. galbana DNA was detected by a non-radiometric alkaline filter elution assay after T4-endonuclease V digestion. The quantity of CPDs was estimated by alkaline agarose gel electrophoresis. Precise determination of the amount of DNA in the presence of I. galbana pigments was achieved by oxazole yellow homodimer (YOYO) dye. T4-endonuclease V-sensitive sites frequency (ESS/kb), measured after exposure to 2-40 kJ m(-2) of artificial UV light, increased in a dose-dependent manner. Twelve hours after…

Technogenic Magnetic Particles in Alkaline Dusts from Power and Cement Plants

2012

During this study, we investigated the mineralogical characterization of technogenic magnetic particles (TMPs) contained in alkaline industrial dust and fly ash emitted by coal burning power plants and cement plants. The reaction of tested dust samples varied between values of pH 8 and pH 12. Their magnetic properties were characterized by measurement of magnetic susceptibility (χ), frequency dependence of magnetic susceptibility (χ(fd)), and temperature dependence of magnetic susceptibility. Mineralogical and geochemical analyses included scanning electron microscopy with energy dispersive spectroscopy, microprobe analysis and X-ray diffraction. The TMPs in fly ash from hard coal combustio…

Determination and comparison of acidic gas ratios at the Stromboli Volcano and Mount Etna obtained by various active alkaline traps

2013

Expression patterns of matrix genes during human skeletal development.

1994

High risk of bacterobilia in advanced experimental chronic fasciolosis

2006

Fasciolosis is recognized as an important human disease. Wistar rats experimentally infected with Fasciola hepatica were examined using data obtained in the advanced chronic state of the disease (200, 300 and 400 days post-infection, dpi). Pigment stones (PS) and bile specimens were collected. The same procedure was applied in control rats. Liver tests were determined using stored serum samples. Bacteriological bile culture revealed viable bacteria (Escherichia coli, 45% of cases, Enterococcus faecalis, 45% and Klebsiella pneumoniae, 10%). The presence of bacterobilia was associated with liver serum enzymes, including aspartate aminotransferase (AST or SGOT), alanine aminotransferase (ALT o…

Volatiles and trace elements content in melt inclusions from the zoned Green Tuff ignimbrite (Pantelleria, Sicily): petrological inferences

2018

International audience; The island of Pantelleria is one of the best known localities of bimodal mafic-felsic magmatism (alkali basalt and trachyte-pantellerite). Among the felsic rocks, the coexistence in a single eruption of products of both trachyte and pantellerite compositions is limited to few occurrences, the Green Tuff (GT) ignimbrite being one of these. The GT is compositionally zoned from pantellerite (70.1 wt% SiO2, mol Na+K/Al = 1.86, 1871 ppm Zr) at the base to crystal-rich (>30 vol%) comenditic trachyte (63.4 wt% SiO2, mol Na+K/Al = 1.10, 265 ppm Zr) at the top, although the pantellertic compositions dominate the erupted volume. We present here new data on melt inclusions (MIs…

Age and evolution of late Mesozoic metamorphic core complexes in southern Siberia and northern Mongolia

2008

Numerous Cretaceous metamorphic core complexes (MCCs) extend from Transbaikalia in Russia to northern Mongolia within the Central Asian Orogenic Belt. We investigated the Buteel and Zagan MCCs in detail. Shear sense indicators in mylonitized rocks show footwall-to-the-NW tectonic transport. Single zircon dating of footwall rocks in the Buteel MCC establishes the emplacement of granitoid orthogneiss precursors at 240–211 Ma, a felsic metavolcanic rock at 265.0 ± 1.2 Ma, a syenite at 265.5 ± 1.2 Ma and a metarhyolite of the pre-granitoid basement at 553.6 ± 2.9 Ma. A peralkaline granite intruding orthogneisses of the Zagan MCC has a new U–Pb zircon age of 151.6 ± 0.7 Ma. 40Ar/39Ar ages of 133…

Effectiveness of sodium acetate treatment on the mechanical properties and morphology of natural fiber-reinforced composites

2021

This paper aims to investigate the ability of an eco-friendly and cheap treatment based on sodium acetate solutions to improve the mechanical properties of flax fiber-reinforced composites. Flax fibers were treated for 5 days (i.e., 120 h) at 25 °C with mildly alkaline solutions at 5%, 10% and 20% weight content of the sodium salt. Quasi-static tensile and flexural tests, Charpy impact tests and dynamical mechanical thermal (DMTA) tests were carried out to evaluate the mechanical properties of the resulting composites. Fourier transform infrared analysis (FTIR) was used to evaluate the chemical modification on the fibers surface due to the proposed treatment, whereas scanning electron micro…

A bio-imitating approach to fabricate an artificial matrix for cartilage tissue engineering using magnesium-polyphosphate and hyaluronic acid

2016

Here we describe an artificial cartilage-like material based on a hyaluronic acid-Mg/Ca-polyphosphate paste (HA-aMg/Ca-polyP-p) that is fabricated from a water-soluble Na-salt of energy-rich inorganic polyphosphate (polyP) and soluble hyaluronic acid in the presence of water-insoluble CaCO3. The resulting material, after conversion of Na-polyP into the less soluble Mg/Ca-salt consisting of amorphous Mg/Ca-polyP microparticles, was found to mimic the physiological cartilage tissue and to bind Ca2+ ions present in the synovial fluid. After the Mg2+/Ca2+ exchange and water extrusion, the polyP becomes more stable, but is still susceptible to hydrolytic cleavage by the alkaline phosphatase (ALP…