Search results for "allozyme"
showing 10 items of 21 documents
Congruence in genetic markers used to describe Mediterranean and Atlantic populations of European hake (Merluccius merluccius L. 1758)
2004
Summary Eight samples of the hake, Merluccius merluccius L., from the Mediterranean basin (370 fishes total) and one from the Atlantic ocean (50 fishes) were analysed in order to assess genetic variability and describe genetic population structure. Five polymorphic protein coding loci were scored (ADH*, PGI-1*, PGI-2*, PGM* and SOD-1*) in eight samples, together with a haplotype variation of four samples, obtained from polymerase chain reaction/restriction fragment length polymorphism (PCR–RFLP) analysis on the mitochondrial DNA control region. The average value for observed heterozygosity was typically higher than expected (showing an excess of heterozygotes among the samples) whereas the …
Allozymic variation in Mediterranean hake, Merluccius merluccius (Gadidae)
1998
Abstract Four hundred and twenty individual hake from 10 sample sites in the Mediterranean Sea were analysed in order to study genetic variability and identify genetic stock structure. Twenty loci were identified, four of which were polymorphic at the 95% level: ADH*, PGI‐1*, PGI‐2* and SOD‐1*. Average observed and expected heterozygosity were 0.084 and 0.090, respectively. PG1–1* deviated from Hardy‐Weinberg expectations due to an excess of heterozygotes and F‐statistic analysis showed also a significant excess of heterozygosity at SOD‐1*. FST was not significant for each locus except for PGI‐2*, where a single sample from the Channel of Sicily (C5) showed a different pattern in allelic fr…
Are there any distinct genetic sub-populations of sand smelt, Atherina boyeri (Teleostei: Atherinidae) along Italian coasts? Evidence from allozyme a…
2007
Two hundred and ninety-nine specimens of Atherina boyeri, collected from five Mediterranean sites (three from the open sea (Chioggia, Catania and Gaeta) and two from river mouths (the Birgi and S. Bartolomeo rivers) were analyzed in order to confirm previous genetic studies. Eigtheen loci were scored from 12 enzymatic systems, 3 of which were polymorphic: EST*, PGM* and PGI*. Mean Fst value (0.300, p< 0.05) indicated a high genetic heterogeneity. Pairwise Fst and genetic distance revealed that samples from the river mouths (Birgi and S. Bartolomeo) were grouped separately from the open sea samples (Catania, Chioggia and Gaeta). These results are in agreement with the most recent classificat…
Patrimonio genetico di Apis mellifera sicula Montagano in Sicilia occidentale ed isole minori.
2002
Artificial environmental conditions can affect allozyme genetic structure of the marine gastropod Patella caerulea
2001
Samples of the Mediterranean limpet Patella caerulea collected from 10 sites were examined for genetic population structure. Six of the 20 identified enzymatic loci were polymorphic. The AAT* locus was polymorphic only in two samples from an artificial environment (TI2 and PE). The proportion of polymorphic loci ranged from 0.20 to 0.30, and the observed and expected mean heterozygosity varied between 0.098 and 0.076 and between 0.109 and 0.086, respectively. Mean Fis values were significantly positive in AAT*, ESTD*, PEPC-2*, and PEPD*, showing heterozygosity deficiency. In all, mean Fst value of 0.007 indicated high genetic homogeneity between the samples analyzed, whereas single-locus Fs…
Biochemical genetic differentiation between Pomatoschistus marmoratus and P. tortonesei
1999
Several diagnostic genetic markers were identified in Pomatoschistus marmoratus and P. tortonesei using polyacrylamide gel electrophoresis (PAGE) of allozymes. Twenty-one loci were resolved, including the electrophoretic pattern of muscle proteins. The MDH*, PGM1,2*, EST-1,2*, FUM* and PGI-2* loci exhibited different alleles which were fixed for the two species being analysed. Genetic distance, as calculated by Nei's index, showed a value of 0.413. Environmental hypersalinity, could have influenced the geographical distribution of P. tortonesei.
Genetic and morphometric variation of Mediterranean hake, Merluccius merluccius, in the Strait of Sicily (Central Mediterranean)
2004
Allozyme, morphometric and growth analyses were applied to samples collected in the Strait of Sicily (central Mediterranean Sea) to test the hypothesis of the existence of a unique hake stock (Merluccius merluccius) in the study area. The level of genetic variation was detected from five polymorphic loci (ADH*, PGI-1*, PGI-2*, PGM*, SOD-1*). The average observed heterozygosity amounted to 0.421, while the average expected was 0.353. Weir & Cockerham statistics showed no heterogeneity, except for the single PGI? locus (theta = 0.011; P 0.05). Morphometric analyses revealed some differentiation. Females showed different relationships in six out of eight morphometric indices with total length,…
Biochemical taxonomic differentiation between Mullus barbatus and mullus surmuletus (Pisces, mullidae)
1991
Abstract 1. 1. The electrophoretic data from seven enzymatic systems, codifying for 20 loci, and the patterns of general proteins from some tissues of Mullus barbatus and Mullus surmuletus were examined. 2. 2. The value of the genetic distance index as per Nei is 0.068, this criterium showing a high similarity between the two species. 3. 3. Species-specific patterns were found for the SOD enzyme and general proteins of the muscle.
Studies on the Sicilian populations ofAnacolia webbii(Mont.) Schimp. (Bartramiaceae, Bryophyta), rare moss in Europe
2014
New data on the distribution, biology, intra- and inter-population variability and conservation state of Sicilian populations of Anacolia webbii are provided. Two new localities are reported, so that total five fragmented populations occur in the island, all of them in a rather small area in the western part of the province of Palermo. They live on wet limestone or sandstone cliffs with base at 900–1200 m a.s.l. and N, N-NE aspect, within communities of Aceri campestris-Querco-ilicis sigmetum. The plants are particularly vigorous, but some data suggest a condition of vulnerability of the populations. In fact, in addition to specificity to a rare habitat on the island, a morphometric and all…
Biochemical genetic differentiation between Pomatoschistus marmoratus and P. tortonesei (Pisces, Gobiidae).
1999
Several diagnostic genetic markers were identified in Pomatoschistus marmoratus and P. tortonesei using polyacrylamide gel electrophoresis (PAGE) of allozymes. Twenty-one loci were resolved, including the electrophoretic pattern of muscle proteins. The MDH*, PGM- 1,2*, EST-1,2*, FUM* and PGI-2* loci exhibited different alleles which were fixed for the two species being analysed. Genetic distance, as calculated by Nei’s index, showed a value of 0·413. Environmental hypersalinity, could have influenced the geographical distribution of P. tortonesei.