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showing 10 items of 29275 documents
Dexamethasone premedication suppresses vaccine-induced immune responses against cancer
2020
ABSTRACT Glucocorticosteroids (GCS) have an established role in oncology and are administered to cancer patients in routine clinical care and in drug development trials as co-medication. Given their strong immune-suppressive activity, GCS may interfere with immune-oncology drugs. We are developing a therapeutic cancer vaccine, which is based on a liposomal formulation of tumor-antigen encoding RNA (RNA-LPX) and induces a strong T-cell response both in mice as well as in humans. In this study, we investigated in vivo in mice and in human PBMCs the effect of the commonly used long-acting GCS Dexamethasone (Dexa) on the efficacy of this vaccine format, with a particular focus on antigen-specif…
Eukaryotic rRNA Modification by Yeast 5-Methylcytosine-Methyltransferases and Human Proliferation-Associated Antigen p120.
2015
International audience; Modified nucleotide 5-methylcytosine (m(5)C) is frequently present in various eukaryotic RNAs, including tRNAs, rRNAs and in other non-coding RNAs, as well as in mRNAs. RNA: m(5)C-methyltranferases (MTases) Nop2 from S. cerevisiae and human proliferation-associated nucleolar antigen p120 are both members of a protein family called Nop2/NSUN/NOL1. Protein p120 is well-known as a tumor marker which is over-expressed in various cancer tissues. Using a combination of RNA bisulfite sequencing and HPLC-MS/MS analysis, we demonstrated here that p120 displays an RNA:m(5)C-MTase activity, which restores m(5)C formation at position 2870 in domain V of 25S rRNA in a nop2 Delta …
Measuring Distraction at the Levels of Tactical and Strategic Control: The Limits of Capacity-Based Measures for Revealing Unsafe Visual Sampling Mod…
2011
The control theory of driving suggests that driver distraction can be analyzed as a breakdown of control at three levels. Common approach for analyzing distraction experimentally is to utilize capacity-based measures to assess distraction at the level of operational control. Three driving simulation experiments with 61 participants were organized to evaluate which kind of measures could be used to analyze drivers' tactical visual sampling models and the related effects of distraction while searching textual information on in-car display. The effects of two different text types were evaluated. The utilized capacity-based measures seemed to be insufficient for revealing participants' tactical…
Visualization in the integrated SimPhoNy multiscale simulation framework
2018
Abstract We describe three distinct approaches to visualization for multiscale materials modelling research. These have been developed with the framework of the SimPhoNy FP7 EU-project, and complement each other in their requirements and possibilities. All have been integrated via wrappers to one or more of the simulation approaches within the SimPhoNy project. In this manuscript we describe and contrast their features. Together they cover visualization needs from electronic to macroscopic scales and are suited to simulations made on personal computers, workstations or advanced High Performance parallel computers. Examples as well as recommendations for future calculations are presented.
An Approach for Network Outage Detection from Drive-Testing Databases
2012
A data-mining framework for analyzing a cellular network drive testing database is described in this paper. The presented method is designed to detect sleeping base stations, network outage, and change of the dominance areas in a cognitive and self-organizing manner. The essence of the method is to find similarities between periodical network measurements and previously known outage data. For this purpose, diffusion maps dimensionality reduction and nearest neighbor data classification methods are utilized. The method is cognitive because it requires training data for the outage detection. In addition, the method is autonomous because it uses minimization of drive testing (MDT) functionalit…
High-precision measurement of the mass difference between 102Pd and 102Ru
2019
Abstract The Q-value for the neutrinoless double electron capture on 102Pd, Qϵϵ(102Pd), is determined as the atomic mass difference between 102Pd and 102Ru. A precise measurement of the Qϵϵ(102Pd) at the SHIPTRAP Penning trap showed a more than 10σ deviation to the adopted Atomic Mass Evaluation (AME) value. The reliability of the SHIPTRAP measurement was challenged because the AME value was based on numerous experiments including β and electron capture decays and very precise (n, γ) data, all agreeing with each other. To solve the discrepancy, the Qϵϵ(102Pd) has now been determined with the JYFLTRAP Penning trap at the IGISOL facility in the Accelerator Laboratory of the University of Jyva…
Effect of Ion Escape Velocity and Conversion Surface Material on H- Production
2011
According to generally accepted models surface production of negative ions depends on ion escape velocity and work function of the surface. We have conducted an experimental study addressing the role of the ion escape velocity on H− production. A converter‐type ion source at Los Alamos Neutron Science Center was employed for the experiment. The ion escape velocity was affected by varying the bias voltage of the converter electrode. It was observed that due to enhanced stripping of H− no direct gain of extracted beam current can be achieved by increasing the converter voltage. The conversion efficiency of H− was observed to vary with converter voltage and follow the existing theories in qual…
Jahn–Teller effects in Au25(SR)18
2016
The relationship between oxidation state, structure, and magnetism in many molecules is well described by first-order Jahn–Teller distortions. This relationship is not yet well defined for ligated nanoclusters and nanoparticles, especially the nano-technologically relevant gold-thiolate protected metal clusters. Here we interrogate the relationships between structure, magnetism, and oxidation state for the three stable oxidation states, −1, 0 and +1 of the thiolate protected nanocluster Au25(SR)18. We present the single crystal X-ray structures of the previously undetermined charge state Au25(SR)18+1, as well as a higher quality single crystal structure of the neutral compound Au25(SR)180. …
JIMWLK and beyond: From concepts to observables
2016
Volume: 112 Host publication title: 6th International Conference on Physics Opportunities at an Electron-Ion Collider The Color Glass Condensate and its associated evolution equation, the JIMWLK equation have applications to many observables far beyond totally inclusive observables. The phenomenology is so rich that little has been done to explore beyond scaling behavior of correlators. We show first examples that exemplify the considerations necessary to access additional information both experimentally and theoretically and demonstrate that the Wilson line correlators appearing throughout make it imperative to consistently take into account that one is dealing with correlators of group el…
SERS detection of cell surface and intracellular components of microorganisms using nano-aggregated Ag substrate
2016
Abstract The intracellular and cell surface composition and structural features of gram-positive and gram-negative bacteria were identified using near-infrared surface-enhanced Raman scattering (SERS). The structural differences of components that reside in the cell envelope are manifested by their SERS spectra, e.g. gram-negative vs. gram-positive. Silver particles were used as a SERS substrate by exploiting the existence of strong local electromagnetic fields (hot spots) within nanoscale aggregates of the particles. The aggregation of silver nanoparticles was induced by magnesium ions. These hot spots reduce the screening length of the double layer. The obtained SERS spectra showed excell…