Search results for "asm"
showing 10 items of 16598 documents
Observation of classically 'forbidden' electromagnetic wave propagation and implications for neutrino detection.
2018
Ongoing experimental efforts in Antarctica seek to detect ultra-high energy neutrinos by measurement of radio-frequency (RF) Askaryan radiation generated by the collision of a neutrino with an ice molecule. An array of RF antennas, deployed either in-ice or in-air, is used to infer the properties of the neutrino. To evaluate their experimental sensitivity, such experiments require a refractive index model for ray tracing radio-wave trajectories from a putative in-ice neutrino interaction point to the receiving antennas; this gives the degree of signal absorption or ray bending from source to receiver. The gradient in the density profile over the upper 200 meters of Antarctic ice, coupled wi…
Chemical and mineralogical analyses on stones from Sagunto Castle (Spain)
2019
Abstract For the first time, an archaeometric study was carried out on the carbonate rock ashlars of the Sagunto Castle. The studied site is one of the most important and best preserved Spanish archaeological and architectural monuments, characterized by different construction phases from the Roman period to Modern Ages. Forty samples collected from thirteen different structures of Sagunto Castle and two quarries, located in the Sagunto's hill were used for comparative purposes. The samples were analyzed by X-ray diffraction, X-ray fluorescence and inductively coupled plasma mass spectrometry to determine their mineralogical and elemental composition. The obtained data show similar chemical…
Dental microwear texture analysis on extant and extinct sharks : Ante- or post-mortem tooth wear?
2020
Sharks are apex-predators that play an important role in past and present aquatic food webs. However, their diet - especially in extinct species - is often not well constrained. Dental microwear texture analysis (DMTA) has been successfully applied to reconstruct diet and feeding behaviours of different aquatic and terrestrial vertebrates. However, unlike in mammals, food-to-tooth contact in sharks is rather limited because only larger prey is manipulated before swallowing. Together with a fast tooth replacement rate, this reduces wear on individual teeth. Here, we present an explorative study of dental microwear texture on extant and extinct sharks to test whether ante-mortem wear is relat…
First shark record from the Upper Cretaceous of the Kuril Islands, Far East Russia
2020
Abstract The first find of a Late Cretaceous shark tooth, or of any cartilaginous fish for that matter, from the Kuril Islands in the Russian Far East is recorded as Carcharias sp. (Lamniformes, Carchariidae). The specimen originates from Maastrichtian strata on the island of Shikotan that are assigned to the Malokurilsk Formation. It constitutes an extremely rare find from rocks of this age in the northwest Pacific region. External and internal dental structures have been reconstructed by the use of computed tomography. The vasculature of this lamniform tooth is first modelled by CT scanning and shown under different angles, which allows an assessment of the spatial arrangement of hierarch…
Mn/Ca in shells of Arctica islandica (Baltic Sea) – A potential proxy for ocean hypoxia?
2021
Oxygen depletion threatens an increasing number of shallow water environments, specifically habitats below the seasonal halocline in coastal settings of the Baltic Sea. To understand the natural variations of dissolved oxygen levels on seasonal and inter-annual time-scales prior to the instrumental era, high-resolution archives are urgently required. The present study evaluates the potential use of Mn/Ca values in shells of the bivalve, Arctica islandica to infer concentrations of past dissolved oxygen concentrations. This study is based on laser ablation – inductively coupled plasma – mass spectrometry (LA-ICP-MS) data of six contemporaneous specimens and demonstrates that background varia…
Recombinant laccase from Pediococcus acidilactici CECT 5930 with ability to degrade tyramine
2017
Biogenic amines degradation by bacterial laccases is little known, so we have cloned and heterologously expressed, in E. coli, a new laccase from Pediococcus acidilactici CECT 5930 (Lpa5930), a lactic acid bacterium commonly found in foods able to degrade tyramine. The recombinant enzyme has been characterized by physical and biochemical assays. Here we report the optimization of expression and purification procedures of this laccase. DNA encoding sequence of laccase from P. acidilactici was amplified by PCR and cloned into the expression plasmid pET28a for induction by isopropyl-β-D-thiogalactoipyranoside. Protein expression was performed in E. coli BL21(DE3) harboring pGro7 plasmid expres…
Transcriptional responses to pre-flowering leaf defoliation in grapevine berry from different growing sites, years, and genotypes
2017
Leaf removal is a grapevine canopy management technique widely used to modify the sourceâsink balance and/or microclimate around berry clusters to optimize fruit composition. In general, the removal of basal leaves before flowering reduces fruit set, hence achieving looser clusters, and improves grape composition since yield is generally curtailed more than proportionally to leaf area itself. Albeit responses to this practice seem quite consistent, overall vine performance is affected by genotype, environmental conditions, and severity of treatment. The physiological responses of grape varieties to defoliation practices have been widely investigated, and just recently a whole genome trans…
Toward the valorization of olive (Olea europaea var. europaea L.) biodiversity: horticultural performance of seven Sicilian cultivars in a hedgerow p…
2019
Abstract An intense survey of the Sicilian’s olive growing areas for autochthonous germplasm, mainly represented by centennials olive trees (Olea europaea var. europaea L.) apparently older then III centuries, started at the beginning of the 1980s and resulted in the selection of more than 150 cultivars and accessions. This germplasm was propagated in a nursery, by grafting onto seedlings of Olea europaea L., and planted in an experimental orchard, in an olive district located in the South-west of the Island, where they were evaluated for over 30 years and selected for their early bearing, high and constant productivity, as well as high oil content of the fruits and excellent chemical (olei…
Genetic Mechanisms of the Devious Intruder Candidatus Liberibacter in Citrus.
2017
HLB symptom progression may result from three types of dysfunction occurring in Ca.L.-infected citrus: (1) a carbohydrate disorder linked to disruption of the source-sink relationship, (2) perturbation of hormonal crosstalk involved in plant immune responses (JA-SA signaling crosstalk), and (3) changes in the rapid activation of detoxifying pathways (particularly GSTs). The development of innovative short- or long-term biotechnological tools that allow beneficial modulation of these three pathways will help increase Citrus tolerance to this devastating disease.
Role of Bacillus thuringiensis Cry1A toxins domains in the binding to the ABCC2 receptor from Spodoptera exigua
2018
Abstract Cry proteins from Bacillus thuringiensis (Bt) have been used to control insect pests either as formulated sprays or as in Bt-crops. However, field-evolved resistance to Bt proteins is threatening the long-term use of Bt products. The SeABCC2 locus has been genetically linked to resistance to a Bt bioinsecticide (Xentari™) in Spodoptera exigua (a mutation producing a truncated form of the transporter lacking an ATP binding domain was found in the resistant insects). Here, we investigated the role of SeABCC2 in the mode of action of Cry1Aa, Cry1Ab, Cry1Ac, Cry1Ca, and two Cry1A-1Ca hybrids by expressing the receptor in Sf21 and HEK293T cell lines. Cell toxicity assays showed that Sf2…