Search results for "assembly"
showing 10 items of 768 documents
Impact of analytic provenance in genome analysis
2014
Background Many computational methods are available for assembly and annotation of newly sequenced microbial genomes. However, when new genomes are reported in the literature, there is frequently very little critical analysis of choices made during the sequence assembly and gene annotation stages. These choices have a direct impact on the biologically relevant products of a genomic analysis - for instance identification of common and differentiating regions among genomes in a comparison, or identification of enriched gene functional categories in a specific strain. Here, we examine the outcomes of different assembly and analysis steps in typical workflows in a comparison among strains of Vi…
Tuning the pH-Switch of Supramolecular Polymer Carriers for siRNA to Physiologically Relevant pH
2017
The preparation of histidine enriched dendritic peptide amphiphiles and their self-assembly into multicomponent pH-switchable supramolecular polymers is reported. Alternating histidine and phenylalanine peptide synthons allow the assembly/disassembly to be adjusted in a physiologically relevant range of pH 5.3-6.0. Coassembly of monomers equipped with dendritic tetraethylene glycol chains with monomers bearing peripheral primary amine groups leads to nanorods with a tunable cationic surface charge density. These surface functional supramolecular polycations are able to reversibly bind short interfering RNA (siRNA). The nanorod-like supramolecular polymers, their complexation with siRNA, and…
Self-assembly, binding ability and magnetic properties of dicopper(ii) pyrazolenophanes
2016
A novel series of dinuclear copper(II) pyrazolenophanes of the formula [Cu2(μ-4-Mepz)2(μ-ClO4)(ClO4)(bpm)2] (1), [Cu2(μ-pz)2(μ-H2O)(ClO4)(4,7-Me2phen)2]ClO4·H2O·CH3CN (2), [Cu2(μ-pz)2(μ-H2O)(ClO4)3/2(H2O)1/2(phen)2]2[Cu2(μ-pz)2(μ-ClO4)(ClO4)2(phen)2]·8H2O (3), and [Cu2(μ-pz)2(CH3CN)2(3,4,7,8-Me4phen)2](ClO4)2 (4) (Hpz = pyrazole, H-4-Mepz = 4-methylpyrazole, bpm = 2,2'-bipyrimidine, phen = 1,10-phenanthroline, 4,7-Me2phen = 4,7-dimethyl-1,10-phenanthroline, and 3,4,7,8-Me4phen = 3,4,7,8-tetramethyl-1,10-phenanthroline) have been synthesized and magneto-structurally investigated. The crystal structures of 1–4 contain bis(pyrazolate)(perchlorate)- (1 and 3), bis(pyrazolate)(aqua)- (2 and 3), …
Three‐Component Self‐Assembly Changes its Course: A Leap from Simple Polymers to 3D Networks of Spherical Host–Guest Assemblies
2021
Angewandte Chemie / International edition 60(21), 12132 - 12142 (2021). doi:10.1002/anie.202103178
Artificially inscribed defects in opal photonic crystals
2005
Opals are three-dimensional photonic crystals, self-assembled from dielectric spherical beads into a face-centered cubic lattice. By introducing intentional defects in the crystal lattice, one modifies features such as spontaneous emission and the directionality of diffracted light. We here present a method for the artificial introduction of a lattice of such intentional defects in self-assembled poly(methyl methacrylate) (PMMA) photonic crystals by means of electron beam lithography. The inscribed defects are of the size of an individual bead, providing a broad spectral range between adjacent resonance peaks. This opens for devices with single line transmission in the photonic band gap, as…
Dynamic Diglyme-Mediated Self-Assembly of Gold Nanoclusters
2015
We report the assembly of gold nanoclusters by the nonthiolate ligand diglyme into discrete and dynamic assemblies. To understand this surprising phenomenon, the assembly of Au20(SC2H4Ph)15-diglyme into Au20(SC2H4Ph)15-diglyme-Au20(SC2H4Ph)15 is explored in detail. The assembly is examined by high-angle annular dark field scanning transmission electron microscopy, size exclusion chromatography, mass spectrometry, IR spectroscopy, and calorimetry. We establish a dissociation constant for dimer to monomer conversion of 20.4 μM. Theoretical models validated by transient absorption spectroscopy predict a low-spin monomer and a high-spin dimer, with assembly enabled through weak diglyme oxygen-g…
Sequencing, De Novo Assembly and Annotation of the Colorado Potato Beetle, Leptinotarsa decemlineata, Transcriptome
2012
Background. The Colorado potato beetle (Leptinotarsa decemlineata) is a major pest and a serious threat to potato cultivation throughout the northern hemisphere. Despite its high importance for invasion biology, phenology and pest management, little is known about L. decemlineata from a genomic perspective. We subjected European L. decemlineata adult and larval transcriptome samples to 454-FLX massively-parallel DNA sequencing to characterize a basal set of genes from this species. We created a combined assembly of the adult and larval datasets including the publicly available midgut larval Roche 454 reads and provided basic annotation. We were particularly interested in diapause-specific g…
Pre-stressed translucent panels made of innovative 3D glass components DSC-integrated, for safety and active building façades
2018
The widespread use of glazed envelopes, especially in high-rise buildings, is pushing building designers and researchers to the development of novel solutions for the improvement of their performance. At the University of Palermo, SBskin. Smart Building Skin s.r.l. (an innovative start up and academic spin off) is prototyping precast, pre- stressed and dry-assembled panels made of 3D glass subcomponents, able to reduce the energy consumption of buildings, to produce clean energy and to maintain high level of mechanical resistance against horizontal forces due to winds and earthquakes. The integration of glass subcomponents with third-generation PV technologies -Dye- Sensitized Solar Cells (…
Beyond material factors? Identity, culture and the foreign and security policy of the EU
2015
The evolution of European integration in the field foreign, security and defence policy and the coordination practices established by EU member states cannot be fully explained by looking at material factors only. The chapter adds to the picture the importance of identity, and, in particular, the we-feeling typical of belonging to a security community and security culture in the evolution of the practices adopted for the international projection of the EU and its member states. In consideration of the importance that support to the UN has acquired since the 2003 ESS, both as a strategic priority and as a constitutive element of the EU identity, the chapter focuses on the coordination practi…
The New Models of Public Policy on Early Childhood Education and Care in Spain
2016
Conceptualizing Early Childhood Education and Care (ECEC) as a right for all young children has gained legitimacy in Spain in recent years (Ancheta, 2008). In 2005, the Committee on the Rights of the Child (CRC) stated that young children have specific needs for nurturing, care and guidance (CRC, 2005), and determined that early childhood education should be provided as the right of children to develop personalities, talents and mental and physical abilities from birth (United Nations General Assembly [UNGA], 2011).