Search results for "assessment."

showing 10 items of 5004 documents

Human Upcyte Hepatocytes: Characterization of the Hepatic Phenotype and Evaluation for Acute and Long-Term Hepatotoxicity Routine Testing

2016

The capacity of human hepatic cell-based models to predict hepatotoxicity depends on the functional performance of cells. The major limitations of human hepatocytes include the scarce availability and rapid loss of the hepatic phenotype. Hepatoma cells are readily available and easy to handle, but are metabolically poor compared with hepatocytes. Recently developed human upcyte hepatocytes offer the advantage of combining many features of primary hepatocytes with the unlimited availability of hepatoma cells. We analyzed the phenotype of upcyte hepatocytes comparatively with HepG2 cells and adult primary human hepatocytes to characterize their functional features as a differentiated hepatic …

0301 basic medicineTime FactorsPrimary Cell CultureTransfectionToxicologyRisk AssessmentTranscriptome03 medical and health sciences0302 clinical medicineMetabolomicsCytochrome P-450 Enzyme SystemIn vivoToxicity TestsmedicineHumansChildGlycogen synthaseDose-Response Relationship DrugbiologyInfant NewbornCytochrome P450Hep G2 CellsMiddle Agedmedicine.diseasePhenotypeHigh-Throughput Screening AssaysIsoenzymesOxidative StressPhenotype030104 developmental biologyGene Expression RegulationLiver030220 oncology & carcinogenesisHepatocytesbiology.proteinHepatic stellate cellCancer researchChemical and Drug Induced Liver InjurySteatosisTranscriptomeToxicological Sciences
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Validación del cribado nutricional Malnutrition Screening Tool comparado con la valoración nutricional completa y otros cribados en distintos ámbitos…

2018

Resumen Introducción: los cribados son empleados para detectar el riesgo nutricional y permiten intervenir precozmente e influir en el pronóstico. La herramienta Malnutrition Screening Tool (MST) está validada en paciente hospitalizado y oncológico ambulatorio. Objetivo: analizar por ámbitos sociosanitarios y grupos etarios los cribados MST, Malnutrition Universal Screening Tool (MUST) y Mini Nutritional Assessment-Short Form (MNA-sf) respecto a la valoración nutricional completa (VNC) como patrón de referencia, y evaluar la utilidad del MST en aquellos ámbitos donde no está validado. Material y métodos: se han incluido 815 sujetos ambulatorios, hospitalizados e institucionalizados. Se les …

0301 basic medicineValidation study030109 nutrition & dieteticsNutrition and DieteticsNutrition assessmentValoración nutricionalbusiness.industryMedicine (miscellaneous)Fiabilidad03 medical and health sciencesMedicineValidezbusinessRiesgo nutricionalCribado nutricionalHumanitiesDesnutrición establecida
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Evaluation of Immunofluorescence Antibody Test Used for the Diagnosis of Canine Leishmaniasis in the Mediterranean Basin: A Systematic Review and Met…

2015

With an expected sensitivity (Se) of 96% and specificity (Sp) of 98%, the immunofluorescence antibody test (IFAT) is frequently used as a reference test to validate new diagnostic methods and estimate the canine leihmaniasis (CanL) true prevalence in the Mediterranean basin. To review the diagnostic accuracy of IFAT to diagnose CanL in this area with reference to its Se and Sp and elucidate the potential causes of their variations, a systematic review was conducted (31 studies for the 26-year period). Three IFAT validation methods stood out: the classical contingency table method, methods based on statistical models and those based on experimental studies. A variation in the IFAT Se and Sp …

0301 basic medicineVeterinary medicinelcsh:MedicineArtificial Gene Amplification and ExtensionPolymerase Chain ReactionMediterranean BasinMathematical and Statistical Techniques0302 clinical medicineZoonosesMedicine and Health SciencesCanine leishmaniasisDog Diseaseslcsh:ScienceLeishmaniasisMammalsMultidisciplinaryGeographymedicine.diagnostic_testMediterranean RegionGeographical regionsRegional geographyResearch AssessmentInfectious DiseasesFluorescent Antibody Technique DirectMeta-analysisVertebratesPhysical Sciencesmedicine.symptomStatistics (Mathematics)Research ArticleNeglected Tropical DiseasesSystematic Reviews030231 tropical medicine030106 microbiologyResearch and Analysis MethodsImmunofluorescenceSensitivity and SpecificityAsymptomatic03 medical and health sciencesDogsparasitic diseasesParasitic DiseasesmedicineAnimalsStatistical MethodsMolecular Biology TechniquesMolecular BiologyProtozoan Infectionsbusiness.industrylcsh:RfungiOrganismsReproducibility of ResultsBiology and Life SciencesLeishmaniasisGold standard (test)Tropical Diseasesmedicine.diseaseEarth sciencesParasitologyAmniotesImmunologyMediterranean BasinParasitologylcsh:QbusinessMathematicsMeta-AnalysisPLOS ONE
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Primary vitrectomy for degenerative and tractional lamellar macular holes: A systematic review and meta-analysis

2021

Purpose To assess the efficacy of vitrectomy in degenerative and tractional lamellar macular holes (LMHs) by meta-analysis of published studies. Methods PubMed, Medline and Embase databases were searched up to May 2020. Included cohorts were divided into three groups: degenerative LMH group, lamellar hole associated epiretinal proliferation (LHEP) group and tractional LMH group. LHEP is likely to be associated with degenerative LMHs, but less commonly could be associated with mixed LMHs. To reduce risk of possible misclassification bias, eyes with LHEP which could not have been precisely classified by the authors, were included into the LHEP group. The primary outcome was to investigate th…

0301 basic medicineVisual acuitygenetic structuresVisionmedicine.medical_treatmentVisual AcuitySocial SciencesVitrectomyDatabase and Informatics MethodsMathematical and Statistical Techniques0302 clinical medicineVitrectomyMedicine and Health SciencesPsychologyMedicineDatabase SearchingMacular holeMultidisciplinaryIncidence (epidemiology)StatisticsQOphthalmic ProceduresRMetaanalysisResearch AssessmentMeta-analysisPhysical SciencesMedicineSensory PerceptionAnatomymedicine.symptomResearch Articlemedicine.medical_specialtySystematic ReviewsScienceSurgical and Invasive Medical ProceduresResearch and Analysis Methods03 medical and health sciencesOcular SystemOphthalmologyHumansStatistical Methodsbusiness.industryPrimary vitrectomyCognitive PsychologyBiology and Life SciencesHumans; Retinal Perforations; Visual Acuity; VitrectomyRetinal Perforationsmedicine.diseaseConfidence intervaleye diseases030104 developmental biology030221 ophthalmology & optometryCognitive ScienceEyesPerceptionVisual gainbusinessHeadMathematicsNeuroscience
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Biomarkers and clinical characteristics of autoimmune chronic spontaneous urticaria: Results of the PURIST Study.

2019

Background: Autoimmune chronic spontaneous urticaria (aiCSU) is an important subtype of chronic spontaneous urticaria (CSU) in which functional IgG autoantibodies to IgE or its high-affinity receptor (FcεRI) induces mast cell degranulation and subsequent symptom development. However, it has not been tightly characterized. This study aimed to better define the clinical and immunological features and to explore potential biomarkers of aiCSU. Methods: This was a multinational, multicenter study of 182 CSU patients. The clinical features studied included: urticaria activity and impact (UAS7 and quality of life); autologous serum skin test (ASST); IgG anti-FcεRI and IgG anti-IgE; IgG-anti-thyrop…

0301 basic medicinechronic spontaneous urticariaMaleBasophilIgG autoantibodiesImmunoglobulin EAutoantigensHistamine Releasechemistry.chemical_compound0302 clinical medicineIron-Binding ProteinsImmunology and AllergyChronic UrticariaReceptorAutologous serum skin testbiologyDegranulationMiddle AgedChronic spontaneous urticariaAntibodies Anti-IdiotypicBasophilsmedicine.anatomical_structurePhenotypeAutologous serum skin testautologous serum skin testFemaleSymptom AssessmentHistamineAdultAdolescentImmunologyIodide PeroxidaseAutoimmune Diseases03 medical and health sciencesYoung Adultautoimmune CSUmedicineJournal ArticleHumansAgedAutoantibodiesbusiness.industryReceptors IgEAutoantibodyBasophil activation030104 developmental biology030228 respiratory systemchemistryImmunoglobulin GImmunologyBasophil activation assaysbiology.proteinAutoimmune CSUbusinessbasophil activation assaysBiomarkersAllergyREFERENCES
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Genome-wide association meta-analysis of corneal curvature identifies novel loci and shared genetic influences across axial length and refractive err…

2020

Corneal curvature, a highly heritable trait, is a key clinical endophenotype for myopia - a major cause of visual impairment and blindness in the world. Here we present a trans-ethnic meta-analysis of corneal curvature GWAS in 44,042 individuals of Caucasian and Asian with replication in 88,218 UK Biobank data. We identified 47 loci (of which 26 are novel), with population-specific signals as well as shared signals across ethnicities. Some identified variants showed precise scaling in corneal curvature and eye elongation (i.e. axial length) to maintain eyes in emmetropia (i.e. HDAC11/FBLN2 rs2630445, RBP3 rs11204213); others exhibited association with myopia with little pleiotropic effects …

0301 basic medicinegenetic structuresMedicine (miscellaneous)EmmetropiaGenome-wide association studyVARIANTSGenome-wide association studiesSensory disorders Donders Center for Medical Neuroscience [Radboudumc 12]Cornea0302 clinical medicineRisk FactorsCorneaDatabases GeneticMULTIPLEMyopiaGene Regulatory NetworksEXPRESSION PATTERNS10. No inequalitylcsh:QH301-705.5POPULATIONGeneticseducation.field_of_studymedicine.diagnostic_testHERITABILITYCorneal DiseasesAsian Continental Ancestry Group ; Axial Length Eye ; Cornea ; Corneal Topography ; Databases Genetic ; European Continental Ancestry Group ; Gene Regulatory Networks ; Genetic Loci ; Genetic Predisposition to Disease ; Genome-Wide Association Study ; Humans ; Myopia ; Phenotype ; Polymorphism Single Nucleotide ; Refractometry ; Risk Assessment ; Risk FactorsCorneal topographyEYE SIZE3. Good healthAxial Length EyePhenotypemedicine.anatomical_structureGeneral Agricultural and Biological SciencesExtracellular matrix organizationKeratoconusCorneal diseasesPopulationBiologyPolymorphism Single NucleotideRisk AssessmentArticleWhite PeopleGeneral Biochemistry Genetics and Molecular BiologyOCULAR COMPONENT DIMENSIONS03 medical and health sciencesSPHERICAL EQUIVALENTAsian PeoplemedicineHumansGenetic Predisposition to DiseaseKERATOCONUS3125 Otorhinolaryngology ophthalmologyeducationCorneal Topographymedicine.diseaseCOLLAGENeye diseasesRefractometry030104 developmental biologylcsh:Biology (General)Genetic LociRE3111 Biomedicinesense organs030217 neurology & neurosurgeryGenome-Wide Association StudyCommunications Biology
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Polymorphisms within the ARNT2 and CX3CR1 Genes Are Associated with the Risk of Developing Invasive Aspergillosis.

2020

Invasive aspergillosis (IA) is a life-threatening infection that affects an increasing number of patients undergoing chemotherapy or allo-transplantation, and recent studies have shown that genetic factors contribute to disease susceptibility. In this two-stage, population-based, case-control study, we evaluated whether 7 potentially functional single nucleotide polymorphisms (SNPs) within the ARNT2 and CX3CR1 genes influence the risk of IA in high-risk hematological patients. We genotyped selected SNPs in a cohort of 500 hematological patients (103 of those had been diagnosed with proven or probable IA), and we evaluated their association with the risk of developing IA. The association of …

0301 basic medicinehost immunityGenotype030106 microbiologyImmunologyPopulationCX3C Chemokine Receptor 1Single-nucleotide polymorphismARNT2 ; CX3CR1 ; genetic susceptibility; host immunity; invasive aspergillosisBiologyAspergillosisMicrobiologyPolymorphism Single NucleotideRisk Assessment03 medical and health sciencesCX3CR1GenotypemedicineGenetic predispositionBasic Helix-Loop-Helix Transcription FactorsHumansGenetic Predisposition to DiseaseARNT2AlleleeducationInvasive Pulmonary Aspergillosiseducation.field_of_studyinvasive aspergillosisHaplotypeAryl Hydrocarbon Receptor Nuclear TranslocatorPCRAGA Study Groupmedicine.diseaseHematologic Diseases3. Good healthSettore MED/15 - MALATTIE DEL SANGUE030104 developmental biologyInfectious DiseasesAspergillusCase-Control StudiesExpression quantitative trait lociImmunologyParasitologygenetic susceptibility
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Development and Validation of an Italian Adaptation of the Psychosocial Aspects of Hereditary Cancer Questionnaire

2021

Individuals that attend cancer genetic counseling may experience test-related psychosocial problems that deserve clinical attention. In order to provide a reliable and valid first-line screening tool for these issues, Eijzenga and coworkers developed the Psychosocial Aspects of Hereditary Cancer (PAHC) questionnaire. The aim of this work was to develop an Italian adaptation of the PAHC (I-PACH). This prospective multicenter observational study included three stages: (1) development of a provisional version of the I-PAHC; (2) pilot studies aimed at testing item readability and revising the questionnaire; and (3) a main study aimed at testing the reliability and validity of the final version …

0301 basic medicinemedia_common.quotation_subjectGenetic counselingContext (language use)030105 genetics & hereditypsychological assessmentgenetic testing03 medical and health sciences0302 clinical medicinemedicinePsychologycancerPsychological testingGeneral PsychologyGenetic testingmedia_commongenetic counselingmedicine.diagnostic_testBrief Research ReportBF1-990cancer; genetic counseling; genetic testing; hereditary cancer; psychological assessmenthereditary cancer030220 oncology & carcinogenesisAnxietyObservational studymedicine.symptomWorryPsychologyPsychosocialClinical psychology
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Psychometric properties of the Persian version of the nutritional form for the elderly (NUFFE) in nursing home residents

2018

Background: The Nutritional Form for the Elderly (NUFFE) is a newly developed tool. This study aimed to carry out a psychometric evaluation of the Persian version of NUFFE (NUFFE-P) among nursing home residents. Methods: Nursing home participant’s aged ≥ 60 years (n=97) were enrolled. The inclusion criteria were residency for at least 6 months in the nursing home, and ability to communicate. Exclusion criteria included cognitive impairment, having depressed mood, severe hearing loss, problems in upper and lower extremities also, history of hospital admission during 6 months before enrollment. Anthropometric measures, laboratory tests, three-day food intake, NUFFE-P version, Mini Nutritional…

0301 basic medicinemedicine.medical_specialty030109 nutrition & dieteticsbusiness.industryIntraclass correlationBarthel indexConcurrent validityGeneral MedicineAnthropometry03 medical and health sciencesElderlySensitivity0302 clinical medicineCronbach's alphaSpecificityPhysical therapyPersian versionMedicineOriginal ArticleGeriatric Depression Scale030212 general & internal medicineNutrition assessmentbusinessNursing homesMedical Journal of the Islamic Republic of Iran
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Circulating microRNAs strongly predict cardiovascular death in patients with coronary artery disease—results from the large AtheroGene study

2016

Introduction Stratification for subsequent coronary events among patients with coronary artery disease (CAD) is of considerable interest because of the potential to guide secondary preventive therapies. Recently, we identified eight microRNAs (miRNAs), which facilitated acute coronary syndrome (ACS) diagnosis. In this study, we aimed to evaluate their potential role as prognostic biomarkers for cardiovascular disease. Methods The serum concentrations of eight candidate miRNAs -miR-19a, miR-19b, miR-132, miR-140-3p, miR-142-5p, miR-150, miR-186, and miR-210 were measured in a cohort of 1112 patients with documented CAD—including 430 patients with ACS and 682 patients with stable angina pecto…

0301 basic medicinemedicine.medical_specialtyAcute coronary syndromePathologyProportional hazards modelbusiness.industryDisease030204 cardiovascular system & hematologymedicine.diseaseCoronary artery disease03 medical and health sciencesCirculating MicroRNA030104 developmental biology0302 clinical medicineInternal medicineCohortmedicineCardiologyBiomarker (medicine)Cardiology and Cardiovascular MedicineRisk assessmentbusinessEuropean Heart Journal
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