Search results for "astrocito"
showing 5 items of 5 documents
Pharmacological blockade of the fatty acid amide hydrolase (FAAH) alters neural proliferation, apoptosis and gliosis in the rat hippocampus, hypothal…
2015
Endocannabinoids participate in the control of neurogenesis, neural cell death and gliosis. The pharmacological effect of the fatty acid amide hydrolase (FAAH) inhibitor URB597, which limits the endocannabinoid degradation, was investigated in the present study. Cell proliferation (phospho-H3(+) or BrdU(+) cells) of the main adult neurogenic zones as well as apoptosis (cleaved caspase-3(+)), astroglia (GFAP(+)), and microglia (Iba1(+) cells) were analyzed in the hippocampus, hypothalamus and striatum of rats intraperitoneally treated with URB597 (0.3 mg/kg/day) at one dose/4-days resting or 5 doses (1 dose/day). Repeated URB597 treatment increased the plasma levels of the N-acylethanolamine…
Ethanol impairs extracellular zinc intake in cultured astrocytes
2009
Zinc (Zn) deficiency is present in many physiological and health problems. Among circumstances involved in Zn deficiency, ethanol consumption appears as a prominent cause. In the CNS substantial amounts of Zn appear accumulated in synaptic vesicles of a particular class of neurons: the Zn enriched neurons very abundant in the telencephalon and cerebral cortex. This is the so called synaptic Zn which is simultaneously released with the neurotransmitter thus exerting a neuromodulator role during synaptic transmission. Neighbour astrocytic processes have to capture the excess of both extracellular Zn and neurotransmitter in order to maintain efficient synaptic transmission between neurons. In …
TSQ fluorochrome incubation increase autometallographic zinc detection in primary cell cultures of astrocytes
2021
In this work, we have analyzed the combination of direct (TSQ) and indirect (AMG) zinc detection methods in order to increase the sensitivity to a better study of the zinc uptake. For that purpose, rat primary cell cultured astrocytes were used. Intracellular Zn levels were visualized by using the TSQ zinc fluorochrome, either in normal conditions or after supplementary addition of 50 µM ZnSO4 to the culture. Fluorescence was recorded with an Olympus microscope BX50WI, equipped with a Hamamatsu ORCA digital camera controlled with the Aquacosmos software. Monolayers were fixed and ionic zinc precipitated by sodium sulphide or sodium selenite. Autometallographic zinc detection was the last st…
Astrocitos y zinc: alteraciones inducidas en modelos in vitro de los síndromes alcohólico fetal y de Down
2015
El zinc es un elemento traza esencial; crítico para un gran número de proteínas estructurales, procesos enzimáticos y factores de transcripción. Esta variada ubicación en las biomoléculas hace que participe en multitud de funciones celulares y tisulares. En el caso particular del cerebro, los iones zinc están implicados, además, en la transmisión sináptica. Por todo ello, la homeostasis del zinc es crucial para la función y supervivencia de las células, y estas han desarrollado una amplia variedad de sistemas para el control de la concentración de zinc en las células. La deficiencia de zinc puede acabar desencadenando muchos problemas fisiológicos y de salud, que afectan a la mayoría de los…
Evaluación y programas de intervención psicoeducativa en niños con tumores en el cerebelo
2003
Se describen las secuelas neurológicas en niños diagnosticados de un tumor en el cerebelo y se ofrecen los resultados de la evaluación de 39 niños, con tumor en el cerebelo, tratados en la Unidad de Oncología Pediátrica del Hospital Infantil “La Fe” de Valencia. Asimismo, se compara la evaluación de secuelas de dos niños de educación infantil afectados de un tumor en el cerebelo: Yolanda (meduloblastoma, PNET) y Santiago (astrocitoma cerebeloso), y se describen las áreas en las que es aconsejable realizar un programa de intervención psicoeducativa.