Search results for "atomic"

showing 10 items of 27529 documents

Redox Imbalance and Mitochondrial Release of Apoptogenic Factors at the Forefront of the Antitumor Action of Mango Peel Extract

2021

Today, an improved understanding of cancer cell response to cellular stress has become more necessary. Indeed, targeting the intracellular pro-oxidant/antioxidant balance triggering the tumor commitment to cell demise could represent an advantageous strategy to develop cancer-tailored therapies. In this scenario, the present study shows how the peel extract of mango—a tropical fruit rich in phytochemicals with nutraceutical properties—can affect the cell viability of three colon cancer cell lines (HT29, Caco-2 and HCT116), inducing an imbalance of cellular redox responses. By using hydro-alcoholic mango peel extract (MPE), we observed a consistent decline in thiol group content, which was a…

0301 basic medicineAntioxidantmedicine.medical_treatmentCellPharmaceutical ScienceOrganic chemistryApoptosisphytochemicalArticleAnalytical Chemistry03 medical and health scienceschemistry.chemical_compound0302 clinical medicineQD241-441Downregulation and upregulationCell Line TumorNeoplasmsDrug DiscoverymedicineHumansViability assayPhysical and Theoretical ChemistryMethyl gallateMembrane Potential MitochondrialMangiferaPlant Extractsmitochondrial apoptogenic proteinsphytochemicalsAntineoplastic Agents PhytogenicBcl-2 family proteinCell biologyMitochondriaBcl-2 family proteins030104 developmental biologymedicine.anatomical_structurechemistryChemistry (miscellaneous)030220 oncology & carcinogenesisCancer cellMolecular MedicineVDAC1Oxidation-ReductionIntracellularmitochondria injuryMolecules
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On the prospect of serum exosomal miRNA profiling and protein biomarkers for the diagnosis of ascending aortic dilatation in patients with bicuspid a…

2018

Background: To determine the impact of circulating miRNA and protein activity on the severity of ascending aortic dilatation in patients with bicuspid (BAV) and tricuspid aortic valve (TAV). Methods: By reverse transcription polymerase chain reaction, exosomal circulating expression levels (versus healthy aorta) of miRNAs and absolute levels of transforming growth factor β (TGF-β), matrix metalloproteinases (MMP-2, -3 and -9), tissue inhibitors (TIMP-1, -2, -3 and -4), and soluble receptors for advanced glycation end products AGEs (sRAGE) were evaluated in ascending dilated aortas of 71 patients with different valve morphotype. Results: Less-dilated ascending aorta exhibited a specific miRN…

0301 basic medicineAortic valveAdultMalePathologymedicine.medical_specialtyBicuspid aortic valveHeart Valve Diseases030204 cardiovascular system & hematologyMatrix metalloproteinaseExosomesCohort Studies03 medical and health sciences0302 clinical medicineBicuspid aortic valveBicuspid Aortic Valve DiseaseGlycationmedicine.arteryAscending aortamedicineHumansProspective StudiesReceptorTissue inhibitorAortaAgedAortabusiness.industryAortic failure Ascending aortic dilatationGene Expression ProfilingTransforming growth factor-βMicroRNAMiddle Agedmedicine.diseaseAortic AneurysmReverse transcription polymerase chain reactionMatrix metalloproteinaseMicroRNAs030104 developmental biologymedicine.anatomical_structureAortic Valvecardiovascular systemFemaleTricuspid ValveCardiology and Cardiovascular MedicinebusinessBiomarkersInternational journal of cardiology
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Hemodynamic Instability in Heart Failure Intensifies Age-Dependent Cognitive Decline

2020

This review attempts to examine two key elements in the evolution of cognitive impairment in the elderly who develop heart failure. First, major left side heart parts can structurally and functionally deteriorate from aging wear and tear to provoke hemodynamic instability where heart failure worsens or is initiated; second, heart failure is a major inducer of cognitive impairment and Alzheimer's disease in the elderly. In heart failure, when the left ventricular myocardium of an elderly person does not properly contract, it cannot pump out adequate blood to the brain, raising the risk of cognitive impairment due to the intensification of chronic brain hypoperfusion. Chronic brain hypoperfus…

0301 basic medicineAortic valveAgingCardiac outputmedicine.medical_specialtyHemodynamicsHeart Part03 medical and health sciences0302 clinical medicineInternal medicineMitral valvemedicineAnimalsHumansCognitive DysfunctionCognitive declineAtrium (heart)Heart Failurebusiness.industryGeneral NeuroscienceHemodynamicsBrainGeneral Medicinemedicine.diseasePsychiatry and Mental healthClinical Psychology030104 developmental biologymedicine.anatomical_structureHeart failureCardiologyGeriatrics and Gerontologybusiness030217 neurology & neurosurgeryJournal of Alzheimer's Disease
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Chronic inflammation: A key role in degeneration of bicuspid aortic valve.

2019

Abstract Introduction Bicuspid aortic valve (BAV) is the most common congenital valvular heart defect resulting from abnormal aortic cusp formation during heart development, where two of the three normal and equal sized cusps fuse into a single large cusp resulting in a two cusps aortic valve. Over the past years, much interest has been given in understanding the pathogenesis of BAV and its complications. In this review, we focused on the role of inflammation, involved in the degeneration of BAV and the development of its complications. Role of inflammation From a pathophysiological point of view, BAV may rapidly progress into aortic stenosis (AS) and is related to aortopathy. Several histo…

0301 basic medicineAortic valveHeart Defects Congenitalmedicine.medical_specialtyBicuspid aortic valveCongenital valvular heart defectHeart Valve DiseasesInflammation030204 cardiovascular system & hematologyPathogenesis03 medical and health sciences0302 clinical medicineBicuspid aortic valveBicuspid Aortic Valve DiseaseInternal medicinemedicineHumanscardiovascular diseasesEndothelial dysfunctionMolecular BiologyInflammationHeart developmentNeovascularization Pathologicbusiness.industryCalcinosisChronic inflammationAortic Valve Stenosismedicine.diseaseStenosis030104 developmental biologymedicine.anatomical_structureAortic ValveChronic Diseasecardiovascular systemCardiologyAortic valve calcificationmedicine.symptombusinessCardiology and Cardiovascular MedicineJournal of molecular and cellular cardiology
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Identification of circumferential regional heterogeneity of ascending thoracic aneurysmal aorta by biaxial mechanical testing

2019

Abstract Ascending thoracic aortic aneurysm (ATAA) in patients with bicuspid aortic valve (BAV) can present an asymmetrical aortic dilatation compared with patients with tricuspid aortic valve (TAV). This pattern of aneurysm dilatation led us to hypothesize that biomechanical differences likely induced by regional heterogeneity of material properties can underlie the observed asymmetric enlargement discrepancies between BAV ATAA and TAV ATAA. This study aimed to characterize the mechanical properties and associated aortic tissue stiffness changes along the circumferential direction of aortic rings collected from surgically-repaired patients with ATAA. Biaxial material testing was performed …

0301 basic medicineAortic valveMaleMaterial parameterBicuspid aortic valveHeart Valve DiseasesAorta Thoracic030204 cardiovascular system & hematologyThoracic aortic aneurysm03 medical and health sciences0302 clinical medicineBicuspid aortic valveAneurysmVascular StiffnessBicuspid Aortic Valve Diseasemedicine.arteryMedicineHumansIn patientMolecular BiologyAgedAged 80 and overAortaAortic Aneurysm Thoracicbusiness.industryAscending aortic aneurysmSettore ING-IND/34 - Bioingegneria IndustrialeAnatomyMiddle Agedmedicine.diseaseSettore FIS/07 - Fisica Applicata(Beni Culturali Ambientali Biol.e Medicin)Tissue stiffness030104 developmental biologymedicine.anatomical_structureAortic Valvecardiovascular systemFemaleTissue stiffnessCardiology and Cardiovascular MedicinebusinessBiaxial testingSettore ICAR/08 - Scienza Delle CostruzioniElastic fiberDilatation Pathologic
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Patterns of ascending aortic dilatation and predictors of surgical replacement of the aorta: A comparison of bicuspid and tricuspid aortic valve pati…

2019

Abstract Background Predictors of thoracic aorta growth and early cardiac surgery in patients with bicuspid aortic valve are undefined. Our aim was to identify predictors of ascending aorta dilatation and cardiac surgery in patients with bicuspid aortic valve (BAV). Methods Forty-one patients with BAV were compared with 165 patients with tricuspid aortic valve (TAV). All patients had LV EF > 50%, normal LV dimensions, and similar degree of aortic root or ascending aorta dilatation at enrollment. Patients with more than mild aortic stenosis or regurgitation were excluded. A CT-scan was available on 76% of the population, and an echocardiogram was repeated every year for a median time of 4 ye…

0301 basic medicineAortic valveMalemedicine.medical_specialtyBicuspid aortic valveHeart Valve Diseases030204 cardiovascular system & hematologyThoracic aorta03 medical and health sciencesAortic aneurysm0302 clinical medicineBicuspid aortic valveAneurysmBicuspid Aortic Valve DiseaseAneurysm; Bicuspid aortic valve; Echocardiography; Repeated measures; Thoracic aortaInternal medicinemedicine.arterymedicineThoracic aortaHumansMolecular BiologyAortaAgedDyslipidemiasAortaTricuspid valvebusiness.industryAortic Valve StenosisMiddle Agedmedicine.diseaseAneurysmStenosis030104 developmental biologymedicine.anatomical_structureRepeated measureEchocardiographyAortic ValveHypertensioncardiovascular systemCardiologyFemaleTricuspid ValveRepeated measuresCardiology and Cardiovascular MedicinebusinessTomography X-Ray ComputedDilatation PathologicFollow-Up Studies
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Culture into perfusion-assisted bioreactor promotes valve-like tissue maturation of recellularized pericardial membrane

2020

Derivation of tissue-engineered valve replacements is a strategy to overcome the limitations of the current valve prostheses, mechanical, or biological. In an effort to set living pericardial material for aortic valve reconstruction, we have previously assessed the efficiency of a recellularization strategy based on a perfusion system enabling mass transport and homogenous distribution of aortic valve-derived “interstitial” cells inside decellularized pericardial material. In the present report, we show that alternate perfusion promoted a rapid growth of valve cells inside the pericardial material and the activity of a proliferation-supporting pathway, likely controlled by the YAP transcrip…

0301 basic medicineAortic valvelcsh:Diseases of the circulatory (Cardiovascular) systemCardiovascular Medicine030204 cardiovascular system & hematologyProtein contentBiomaterials03 medical and health sciences0302 clinical medicineBioreactormedicinePericardiumEngineered tissueOriginal ResearchDecellularizationChemistryPerfusion systemBiomaterialValve interstitial cell030104 developmental biologymedicine.anatomical_structureMembranelcsh:RC666-701Valve implantCardiology and Cardiovascular MedicinePerfusionPericardiumBiomedical engineering
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Apolipoprotein B is regulated by gonadotropins and constitutes a predictive biomarker of IVF outcomes

2016

Background Follicular fluid (FF) is an important micro-environment influencing oocyte growth, its development competence, and embryo viability. The FF content analysis allows to identify new relevant biomarkers, which could be predictive of in vitro fertilization (IVF) outcomes. Inside ovarian follicle, the amount of FF components from granulosa cells (GC) secretion, could be regulated by gonadotropins, which play a major role in follicle development. Methods This prospective study included 61 female undergoing IVF or Intra-cytoplasmic sperm injection (ICSI) procedure. Apolipoprotein B (APOB) concentrations in follicular fluid and APOB gene and protein expression in granulosa cells from rep…

0301 basic medicineApolipoprotein Bmedicine.medical_treatment[SDV]Life Sciences [q-bio]Human follicular fluidOocyte RetrievalChorionic GonadotropinHuman chorionic gonadotropinFollicle-stimulating hormone0302 clinical medicineEndocrinologyProspective Studies030219 obstetrics & reproductive medicinebiologyObstetrics and Gynecology[SDV] Life Sciences [q-bio]Treatment Outcomemedicine.anatomical_structureFemalelipids (amino acids peptides and proteins)Apolipoprotein BEmbryo qualityAdultmedicine.medical_specialtyendocrine systemEmbryonic DevelopmentFertilization in Vitro03 medical and health sciencesOvulation InductionInternal medicinemedicineIVF outcomesHumansOvarian follicleApolipoproteins BHuman granulosa cellsGranulosa CellsIn vitro fertilisation[ SDV ] Life Sciences [q-bio]ResearchEmbryo MammalianOocyteFollicular fluidFollicular Fluid030104 developmental biologyEndocrinologyReproductive MedicineFertilizationbiology.proteinFollicle Stimulating HormoneBiomarkersGonadotropinsDevelopmental BiologyReproductive Biology and Endocrinology
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Myeloid-Specific Deletion of Diacylglycerol Lipase α Inhibits Atherogenesis in ApoE-Deficient Mice

2016

BACKGROUND:The endocannabinoid 2-arachidonoylglycerol (2-AG) is a known modulator of inflammation. Despite its high concentration in vascular tissue, the role of 2-AG in atherogenesis has not yet been examined. METHODS:ApoE-deficient mice were sublethally irradiated and reconstituted with bone marrow from mice with a myeloid-specific knockout of the 2-AG synthesising enzyme diacylglycerol lipase α (Dagla) or control bone marrow with an intact 2-AG biosynthesis. After a cholesterol-rich diet for 8 weeks, plaque size and plaque morphology were examined in chimeric mice. Circulating inflammatory cells were assessed by flow cytometry. Aortic tissue and plasma levels of endocannabinoids were mea…

0301 basic medicineApolipoprotein Emedicine.medical_specialtyMyeloidDiacylglycerol lipaselcsh:MedicineInflammationBlood Pressure030204 cardiovascular system & hematologyReceptor Cannabinoid CB203 medical and health sciencesMice0302 clinical medicineApolipoproteins EHeart RateSuperoxidesInternal medicinemedicineAnimalsMyeloid Cellslcsh:ScienceReceptorVascular tissueMice KnockoutLipoprotein lipaseMultidisciplinarybiologyMacrophageslcsh:RAtherosclerosisEndocannabinoid systemPlaque AtheroscleroticLipoprotein Lipase030104 developmental biologymedicine.anatomical_structureEndocrinologyBiochemistrybiology.proteinlcsh:Qlipids (amino acids peptides and proteins)medicine.symptomResearch ArticlePLoS ONE
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Disruption of the CCL1-CCR8 axis inhibits vascular Treg recruitment and function and promotes atherosclerosis in mice

2019

The CC chemokine 1 (CCL1, also called I-309 or TCA3) is a potent chemoattractant for leukocytes that plays an important role in inflammatory processes and diseases through binding to its receptor CCR8. Here, we investigated the role of the CCL1-CCR8 axis in atherosclerosis. We found increased expression of CCL1 in the aortas of atherosclerosis-prone fat-fed apolipoprotein E (Apoe)-null mice; moreover, in vitro flow chamber assays and in vivo intravital microscopy demonstrated an essential role for CCL1 in leukocyte recruitment. Mice doubly deficient for CCL1 and Apoe exhibited enhanced atherosclerosis in aorta, which was associated with reduced plasma levels of the anti-inflammatory interle…

0301 basic medicineApolipoprotein Emedicine.medical_specialtyRegulatory T cellCCL1030204 cardiovascular system & hematologyCCR8T-Lymphocytes RegulatoryReceptors CCR8Chemokine CCL1Mice03 medical and health sciencesApolipoproteins ETh2 Cells0302 clinical medicineInternal medicineLeukocytesmedicineSplenocyteAnimalsMolecular BiologyInflammationMice KnockoutChemistryTh1 CellsAtherosclerosisInterleukin-10Mice Inbred C57BLTregInterleukin 10030104 developmental biologyEndocrinologymedicine.anatomical_structureIL-10CytokinesCardiology and Cardiovascular MedicineCCR8Intravital microscopyCCL1LipoproteinJournal of Molecular and Cellular Cardiology
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