Search results for "autoantibodie"

showing 10 items of 294 documents

Concurrent autoimmune diseases in patients with autoimmune hepatitis.

2010

Although the pathomechanisms of autoimmune diseases in various organs remain unresolved, an accumulation of autoimmune diseases in individual patients has been observed. An overlap of autoimmune hepatitis (AIH) and primary biliary cirrhosis (PBC) or primary sclerosing cirrhosis has been well documented. However, the overlap of autoimmune diseases other than PBC or PSC has not yet been investigated in a large cohort.The goal of our analysis was to investigate the incidence of concurrent autoimmune diseases in patients with AIH.We analyzed our cohort of 278 patients with AIH for concurrent autoimmune diseases.A total of 111 patients (40%) were diagnosed with additional autoimmune diseases. Be…

AdultMaleCirrhosisAdolescentAutoimmune hepatitisThyroiditisAutoimmune DiseasesCohort StudiesYoung AdultPrimary biliary cirrhosisImmunopathologyMedicineHumansIn patientChildAgedAutoantibodiesHepatitisAutoimmune diseaseAged 80 and overbusiness.industryLiver Cirrhosis BiliaryGastroenterologyThyroiditis AutoimmuneMiddle Agedmedicine.diseasedigestive system diseasesHepatitis AutoimmuneImmunologyFemalebusinessFollow-Up StudiesJournal of clinical gastroenterology
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Increased frequency of the CTLA-4 49 A/G polymorphism in patients with acquired haemophilia A compared to healthy controls

2007

Acquired haemophilia (AH) is an autoimmune disorder characterized by autoantibodies against endogenous factor VIII (FVIII). Half of the patients present with an underlying disease known to cause the FVIII autoantibodies whereas in the other half the disease is of idiopathic nature. Recently, it has been shown that variants of the polymorphic cytotoxic T lymphocyte antigen-4 (CTLA-4) gene are associated with autoimmune diseases and also represent a risk factor for inhibitor formation in inherited haemophilia A. In the present study, we investigated whether CTLA-4 variants also play a role in the pathogenesis of AH. Therefore, we analyzed three single nucleotide polymorphisms (SNPs) of the CT…

AdultMaleGenotypeSingle-nucleotide polymorphismHemophilia AHaemophiliaPolymorphism Single NucleotideGene FrequencyAntigens CDGenotypemedicineHumansCTLA-4 AntigenGenetic Predisposition to DiseaseAlleleAllele frequencyGenetics (clinical)AgedAutoantibodiesAged 80 and overAutoimmune diseaseFactor VIIIbusiness.industryAutoantibodyHematologyGeneral MedicineMiddle Agedmedicine.diseaseAntigens DifferentiationCase-Control StudiesImmunologyFemaleGene polymorphismbusinessHaemophilia
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A prospective, double-blind, placebo-controlled trial to establish a safe gluten threshold for patients with celiac disease

2007

BACKGROUND: Treatment of celiac disease (CD) is based on the avoidance of gluten-containing food. However, it is not known whether trace amounts of gluten are harmful to treated patients. OBJECTIVE: The objective was to establish the safety threshold of prolonged exposure to trace amounts of gluten (ie, contaminating gluten). DESIGN: This was a multicenter, double-blind, placebo-controlled, randomized trial in 49 adults with biopsy-proven CD who were being treated with a gluten-free diet (GFD) for > or =2 y. The background daily gluten intake was maintained at < 5 mg. After a baseline evaluation (t0), patients were assigned to ingest daily for 90 d a capsule containing 0, 10, or 50 mg glute…

AdultMaleGluten free diet Celiac Diseasemedicine.medical_specialtyGlutensPlacebo-controlled studyMedicine (miscellaneous)gastroenterologyDiseasedigestive systemGastroenterologyCoeliac diseaselaw.inventionRandomized controlled trialDouble-Blind Methodgluten-free dietlawInternal medicineImmunopathologyIntestine SmallMedicineHumansgluten toxicitysmall-intestinal morphometryLymphocyte CountProspective StudiesIntestinal MucosaProspective cohort studyAutoantibodiesceliac disease; gastroenterology; gluten threshold in gluten-free food; gluten threshold in gluten-free food.; gluten toxicity; gluten-free diet; small-intestinal morphometrychemistry.chemical_classificationNutrition and DieteticsDose-Response Relationship Drugbusiness.industrynutritional and metabolic diseasesMiddle Agedmedicine.diseaseGlutendigestive system diseasesgluten threshold in gluten-free foodchemistryToxicityFemalebusinessceliac disease
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Randomized trial of intravenous immunoglobulins versus prednisolone in Graves' ophthalmopathy.

1996

Abstract Glucocorticoids are usually given for management of Graves' ophthalmopathy (GO), but they may cause side effects. By comparison, intravenous administration of immunoglobulins resulted in clinical improvement and decreased antibody titres in a large number of autoimmune diseases. Therefore, a randomized trial was done, in which 19 patients with active GO were treated with a 20-week course of oral prednisolone (P, starting dose 100 mg/day), and 21 received 1 g immunoglobulin/kg body weight for 2 consecutive days every 3 weeks. The immunoglobulin course was repeated six times. Before and at the end (20 weeks) of immunomodulating therapy, ophthalmological investigation and quantitative…

AdultMaleIntraocular pressureThyroid HormonesVisual acuityEye DiseasesGraves' diseasePrednisoloneImmunologyAdministration OralThyroglobulinlaw.inventionGraves' ophthalmopathyRandomized controlled triallawOral administrationmedicineImmunology and AllergyHumansProspective StudiesGlucocorticoidsAutoantibodiesbusiness.industryImmunoglobulins IntravenousReceptors ThyrotropinOriginal ArticlesMiddle Agedmedicine.diseaseMagnetic Resonance ImagingAnti-thyroid autoantibodiesGraves DiseaseImmunologyPrednisoloneFemalemedicine.symptombusinessmedicine.drugClinical and experimental immunology
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Analysis of Autoantibodies Against Human Retinal Antigens in Sera of Patients with Glaucoma and Ocular Hypertension

2008

The aim of this study was to show that complex antibody patterns against retinal antigens in sera of patients with glaucoma, found in previous studies, are autoantibodies against human antigens.Sera of 179 patients were collected at the Department of Ophthalmology (University of Mainz, Germany): non-glaucomatous control patients (n=45), primary open-angle glaucoma (n=45), ocular hypertension (n=44), and normal tension glaucoma patients (n=45). The sera were tested against Western blots of human retinal antigens. IgG antibody patterns were analyzed by multivariate statistical techniques, and some significant antigens were identified by mass spectrometry.All subjects, even healthy ones, showe…

AdultMaleIntraocular pressuregenetic structuresBlotting WesternGlaucomaOcular hypertensionAutoantigensMass SpectrometryRetinaImmunoglobulin GHistonesTonometry OcularCellular and Molecular NeuroscienceAntigenNormal tension glaucomamedicineHumansIntraocular PressureAgedAutoantibodiesAged 80 and overArrestinbiologybusiness.industryAutoantibodyMiddle Agedmedicine.diseaseeye diseasesSensory SystemsOphthalmologyImmunoglobulin GImmunologybiology.proteinFemaleOcular HypertensionAntibodyCarrier ProteinsbusinessGlaucoma Open-AngleCurrent Eye Research
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Screening for celiac disease in non-Hodgkin's lymphoma patients: a serum anti-transglutaminase-based approach

2003

Several studies have shown the existence of an association between celiac disease (CD) and non-Hodgkin's lymphoma (NHL). Our aim was to evaluate the usefulness of the serum anti-tissue transglutaminase (anti-tTG) antibody assay in screening for CD in consecutive NHL patients. In all, 80 consecutive patients (median age 61 years) with a new diagnosis of NHL were included. To compare the frequency of CD and of positive results for the anti-tTG assay, we enrolled 500 blood donors. In all patients serum anti-tTG was determined with two different ELISA: one based on tTG from guinea pig (gp-tTG) and the other based on human recombinant t-TG (h-tTG) as the antigens. Serum anti-endomysial antibodie…

AdultMaleLymphoma B-CellSettore MED/09 - Medicina InternaAdolescentDuodenumBiopsyGuinea PigsImmunoenzyme TechniqueLymphoma T-CellGliadinGuinea PigImmunoenzyme TechniquesAnimalsHumansIntestinal MucosaFluorescent Antibody Technique IndirectAutoantibodiesAgedNeoplasm StagingAged 80 and overTransglutaminasesAnimalLymphoma Non-HodgkinMiddle AgedAutoantibodieImmunoglobulin ACeliac DiseaseFemaleHuman
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Enhancement of endothelial cell E-selectin expression by sera from patients with active Behçet's disease: moderate correlation with anti-endothelial …

1999

We studied the in vitro E-selection expression of endothelial cells treated with sera from patients with Behcet's disease (BD) and factors (anti-endothelial cell antibodies, anti-neutrophil cytoplasmic antibodies, cytokines, and myeloperoxidase (MPO) that may contribute to adhesion molecule expression. A total of 21 patients with BD and 27 healthy controls were studied. In vitro E-selectin endothelial cell expression was investigated by ELISA after HUVEC incubation with sera or purified IgG from patients with BD and controls. Increased E-selectin expression was observed when endothelial cells were incubated with sera from patients with active disease or from patients with circulating anti-e…

AdultMaleNeutrophilsImmunologyCellIn Vitro TechniquesImmunoglobulin GAntibodies Antineutrophil CytoplasmicE-selectinmedicineImmunology and AllergyHumansCells CulturedAgedAutoantibodiesPeroxidasebiologyCell adhesion moleculeBehcet SyndromeAutoantibodyMiddle AgedMolecular biologyEndothelial stem cellmedicine.anatomical_structureMyeloperoxidaseImmunoglobulin GImmunologybiology.proteinCytokinesFemaleEndothelium VascularAntibodyE-SelectinClinical immunology (Orlando, Fla.)
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The effect of olive oil polyphenols on antibodies against oxidized LDL. A randomized clinical trial

2010

summary Background & aim: Oxidized LDL (oxLDL) is a highly immunogenic particle that plays a key role in the development of atherosclerosis. Some data suggest a protective role of OxLDL autoantibodies (OLAB) in atherosclerosis. Our aim was to assess the effect of olive oil polyphenols on the immunogenicity of oxLDL to autoantibody generation. Methods: In a crossover, controlled trial, 200 healthy men were randomly assigned to 3-week sequences of 25 mL/day of 3 olive oils with high (366 mg/kg), medium (164 mg/kg), and low (2.7 mg/kg) phenolic content. Results: Plasma OLAB concentration was inversely associated with oxLDL (p < 0.001). Olive oil phenolic content increased OLAB generation, with…

AdultMaleOxidized low density lipoproteinCritical Care and Intensive Care MedicineOxidative damageYoung AdultTandem Mass SpectrometryHumansPlant OilsMedicineFood scienceOlive OilChromatography High Pressure LiquidAutoantibodiesCross-Over StudiesNutrition and Dieteticsbiologybusiness.industryAutoantibodyPolyphenolsMiddle AgedLipoproteins LDLOxidative StressBiochemistryPolyphenolLinear Modelsbiology.proteinlipids (amino acids peptides and proteins)Lipid PeroxidationAntibodybusinessOxidized ldlOlive oilClinical Nutrition
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Antibodies to cytoskeletal proteins in patients with Crohn's disease

1990

. The immunologic basis of inflammatory bowel disease has been the focus of interest of a series of studies on Crohn's disease and the process of immune sensitization at the gastrointestinal mucosal level is functionally poorly understood. To date only few contradictory reports concerning the incidence of autoantibodies in patients with this disease exist. The aim of this study was to investigate the sera drawn from 60 patients suffering from biopsy-proven Crohn's disease to evaluate the prevalence of autoantibodies against nuclear antigens and cytoskeletal proteins. Using standard methods, no anti-nuclear antibodies or antibodies to extractable nuclear antigens could be detected. All sera …

AdultMalePathologymedicine.medical_specialtyAdolescentAnti-nuclear antibodyExtractable nuclear antigensClinical BiochemistryBiochemistryInflammatory bowel diseaseImmune systemCrohn DiseaseAntigenmedicineHumansChildAgedAutoantibodiesbiologyAutoantibodyGeneral MedicineMiddle Agedmedicine.diseaseAnti-thyroid autoantibodiesImmunoglobulin ACytoskeletal ProteinsImmunoglobulin MAntibodies AntinuclearImmunoglobulin GImmunologybiology.proteinFemaleAntibodyEuropean Journal of Clinical Investigation
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Two different subtypes of antimitochondrial antibodies are associated with primary biliary cirrhosis: identification and characterization by radioimm…

1987

Antimitochondrial antibodies from patients with primary biliary cirrhosis react with different mitochondrial polypeptides as demonstrated by Western blots. The IgG fractions of a patient with primary biliary cirrhosis Stage I reacting exclusively with a pair of polypeptides at 48,000 daltons (p 48) on Western blot and from a patient with Stage III primary biliary cirrhosis reacting exclusively with a single 62,000 dalton polypeptide (p 62) were labeled with 125I; two radio-immunoassays were established detecting antimitochondrial antibodies against p 62 and p 48, respectively. Autologous sera blocked the assay, but the two reference sera did not block each other. Fourteen of 40 patients wit…

AdultMalePathologymedicine.medical_specialtyAdolescentBiliary cirrhosisRadioimmunoassayMitochondria LiverBiologyPrimary biliary cirrhosisWestern blotAntigenmedicineHumansAgedAutoantibodiesAged 80 and overHepatologymedicine.diagnostic_testStaining and LabelingLiver Cirrhosis BiliaryAutoantibodyRadioimmunoassayMiddle Agedmedicine.diseaseMolecular biologyBlotbiology.proteinImmunologic TechniquesFemaleAntibodyHepatology (Baltimore, Md.)
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