Search results for "aviation.aircraft_model"
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Female sexual signaling in a capital breeder, the European glow-worm Lampyris noctiluca
2021
AbstractTheory predicts that because costs constrain female sexual signaling, females are expected to have a low signaling effort that is increased with passing time until mating is secured. This pattern of signaling is expected to result from females balancing the costs associated with a higher than optimal signaling effort and those costs associated with a low signaling effort that increase the likelihood of delayed mating. We tested whether this prediction applies in the common glow-worm Lampyris noctiluca (Coleoptera, Lampyridae), a capital breeding species in which females glow at night to attract males. Contrary to predictions, we found that the duration of female sexual signaling sig…
Expression and purification of polyhistidine-tagged firefly luciferase in insect cells
2001
The coleopteran firefly, Photinus pyralis, luciferase was produced in lepidopteran Trichoplusia ni insect cells using a baculovirus expression vector. The recombinant protein was equipped with a polyhistidine affinity tag at the carboxyl terminus and purified by immobilized metal-ion affinity chromatography in combination with an expanded bed adsorption system. This approach enabled an efficient, one-step purification protocol of a genetically modified luciferase with properties similar to those of the authentic counterpart. According to light emission measurements, the final yield of highly purified protein was 23 mg l−1 of cell culture. In addition, no specific interaction of interfering …