Search results for "babesia"

A large-scale screening for the taiga tick, Ixodes persulcatus, and the meadow tick, Dermacentor reticulatus, in southern Scandinavia, 2016

2019

Abstract The taiga tick, Ixodes persulcatus, has previously been limited to eastern Europe and northern Asia, but recently its range has expanded to Finland and northern Sweden. The species is of medical importance, as it, along with a string of other pathogens, may carry the Siberian and Far Eastern subtypes of tick-borne encephalitis virus. These subtypes appear to cause more severe disease, with higher fatality rates than the central European subtype. Until recently, the meadow tick, Dermacentor reticulatus, has been absent from Scandinavia, but has now been detected in Denmark, Norway and Sweden. Dermacentor reticulatus carries, along with other pathogens, Babesia canis and Rickettsia r…

Hemoprotozoa of domestic animals in France: Prevalence and molecular characterization

2008

Very limited information is available on epizootiology of haematozoan infections in French domestic animals. In an attempt to address this issue, prevalence of piroplasmida was studied in carnivores and ruminants, whereas prevalence of Hepatozoon spp. was only investigated in carnivores. In total, 383 animals were included in the survey (namely 116 cats, 108 dogs, 91 sheep and 68 cows). Parasite diagnosis was carried out using molecular methods such as PCR and sequencing of the 18S rRNA gene. In addition, ruminant samples were analyzed with the reverse line blotting technique (RLB). Results of RLB and PCR plus sequencing were in total agreement. In carnivores, haematozoan prevalence was clo…

Sympatric Ixodes-tick species: pattern of distribution and pathogen transmission within wild rodent populations

2018

AbstractThe generalist tick Ixodes ricinus is the most important vector for tick-borne pathogens (TBP), including Borrelia burgdorferi sensu lato, in Europe. However, the involvement of other sympatric Ixodes ticks, such as the specialist vole tick I. trianguliceps, in the enzootic circulations of TBP remains unclear. We studied the distribution of I. ricinus and I. trianguliceps in Central Finland and estimated the TBP infection likelihood in the most common rodent host in relation with the abundance of the two tick species. Ixodes trianguliceps was encountered in all 16 study sites whereas I. ricinus was frequently observed only at a quarter of the study sites. The abundance of I. ricinus…

Monitoring of ticks and tick-borne pathogens through a nationwide research station network in Finland.

2020

In 2015 a long-term, nationwide tick and tick-borne pathogen (TBP) monitoring project was started by the Finnish Tick Project and the Finnish Research Station network (RESTAT), with the goal of producing temporally and geographically extensive data regarding exophilic ticks in Finland. In the current study, we present results from the first four years of this collaboration. Ticks were collected by cloth dragging from 11 research stations across Finland in May September 2015-2018 (2012-2018 in Seili). Collected ticks were screened for twelve different pathogens by qPCR: Borrelia afzelii, Borrelia garinii, Borrelia valaisiana, Borrelia burgdorferi sensu stricto, Borrelia miyamotoi, Babesia sp…

Characterization of a novel Babesia bigemina gene and its potential use as molecular marker for strain discrimination

2008

Babesia bovis and B. bigemina diffusion in Eastern Sicily

2007

Cats as reservoir of Babesia microti

2007

Sequence variability in the Babesia bigemina merozoite surface antigen GP45 gene among Mexican and Italian isolates

2008

A promising new ELISA diagnostic test for cattle babesiosis based on Babesia bigemina Apical Membrane Antigen-1.

2016

Babesiosis due to Babesia bigemina is a relevant tick‑borne disease, affecting cattle worldwide. Many surface proteins of the pathogen including the Apical Membrane Antigen 1 (AMA‑1) ‑ have been analysed for vaccine and diagnostic purposes. This study focused on B. bigemina AMA‑1 and on its use for the assessment of diagnostic tests. After bioinformatic analyses, AMA‑1 codifying region was amplified and cloned into an expression vector used to induce protein synthesis in Escherichia coli cells. AMA‑1 was purified by affinity chromatography and used to set up the best condition for an ELISA protocol. Bovine field sera positive to B. bigemina were used to evaluate the presence of anti‑AMA‑1 a…

SVILUPPO DI METODICHE PER LA DIAGNOSI DELLA BABESIOSI BOVINA E RICERCA DI CANDIDATI VACCINALI IN BABESIA BIGEMINA

2012

Cattle babesiosis is a tick-borne disease transmitted by haemoparasites belonging to the phylum of Apicomplexa, such as Babesia bovis and B.bigemina. The pathology affects cattle mainly in tropical and subtropical areas, but also in Europe, strongly reducing meat and milk production. The study performed during these three years of PhD was addressed to the molecular characterization of Italian B.bigemina strains isolated from infected animals, with attention to the genes codifying for surface antigens, putative candidates for vaccine and diagnostic tools development. One of the molecules potentially involved in the erythrocyte invasion by B.bigemina is the Apical Membrane Antigen-1 (AMA-1), …