Search results for "bacteria."
showing 10 items of 4757 documents
Evolution of indigenous starter microorganisms and physicochemical parameters in spontaneously fermented beef, horse, wild boar and pork salamis prod…
2020
Abstract The present work was carried out to evaluate the microbiological and physicochemical composition of salamis produced with the meat of beef, horse, wild boar and pork. Salami productions occurred under controlled laboratory conditions to exclude butchery environmental contaminations, without the addition of nitrate and nitrite. All trials were monitored during the ripening (13 °C and 90% relative humidity) extended until 45 d. The evolution of physicochemical parameters showed that beef and pork salamis were characterized by a higher content of branched chain fatty acids (FA) and rumenic acid than horse and wild boar salamis, whereas the last two productions showed higher values of …
Molecular characterization of dominant bacterial population in “Vastedda della Valle del Belice” cheese: preliminary investigation
2010
The sensory characteristics of raw-milk cheeses are linked to the cheese-making process, to the environmental factors as animal feeding and to the biochemical and microbiological composition of the milk. In this report we temped to characterize the microflora in the typical Sicilian historical cheese as Vastedda della valle del Belice. Each cheese was previous subjected to microbial isolation on specific media (M17 and MRS). The colony obtained on the solid medium were subject to biochemical tests and DNA extraction. The microbial diversity occurring in the strains was evaluated by PCR, RFLP and sequencing targeted on 16S ribosomal DNA. A number of closest relatives species of lactic and co…
BATTERI LATTICI CON ATTIVITA’ ANTIBATTERICA ISOLATI DA FORMAGGI TRADIZIONALI SICILIANI
2013
The study was conducted on typical Sicilian cheeses made from raw cow milk (Caciocavallo Palermitano) and sheep milk (Vastedda della valle del Belice PDO e Pecorino PDO). Genotypic identification of the LAB isolates was carried out by 16S/23S rRNA sequencing. a total of 400 bacteria strains were isolated and genotypically identified as Lactobacillus spp., Lactoccoccus spp., Pediococcus spp., Leuconostoc spp., Enterococcus spp. and Streptococcus spp. Evaluation of bacteriocin-producing LAB was investigated in vitro using the “spot on the lawn” method (2) aganist Listeria monocytogenes, Staphylococcus aureus, Salmonella enteritidis and Escherichia coli. Results showed a predominance of LAB sh…
EVALUATION OF BACTERIAL AND FUNGAL LOAD IN FRESH, FROZEN AND DRIED FOOD MUSHROOMS
2015
The analysis carried out by Doores et al. (1) and Venturini et al. (2) on the total bacterial load tested in wild and cultivated fresh mushrooms highlight values ranging between 3.7 and 9.3 log ufc/g. Due to the absence of pathogens, the microbiological quality of mushrooms analysed by the above-mentioned authors has been considered good. On the basis of data reported in literature we have also extended the observations with frozen and dried mushrooms in order to evaluate the mesophilous bacterial and fungal load. In particular the presence/absence of Escherichia coli (Migula,1895) Castellani & Chalmers, 1919, Salmonella spp. and Listeria monocytogenes (Murray et al.1926) Pirie 1940 was…
Bioactive compounds from brown algae inhabiting the north-western Mediterranean Sea
2021
Marine algae produce a great amount of secondary metabolites (e.g. terpenoids, fatty acids, steroids, polysaccharides and polyphenols) to face environmental stressors they are exposed to, but also to defend themselves from biological pressures such as competitors, pathogens, grazers and epiphytes. Over the last decade, secondary metabolites isolated from brown algae and phenolic compounds, in particular, are receiving increasing attention due to their putative ecological roles, and several biological properties. Brown fucoid algae of the genus Cystoseira sensu latu (Sargassaceae) and Dictyotaceae produce a wide variety of secondary metabolites which have been investigated mainly for their d…
Discovery of plants and fungi with antibacterial activity against Propionibacterium acnes
2016
Acne vulgaris is a common skin disorder that affects >85% of teenagers. Propionibacterium acnes is implicated in its pathogenesis, and like many other bacteria, is subject to emerging trends in antibiotic resistance. Novel therapies are in high demand. The aim of this study was to identify new leads for future pre-clinical development from herbal skin remedies used in Italy. We screened a unique natural product library composed of extracts from medicinal and randomly collected plants and fungi from the Mediterranean for growth-inhibitory and anti-biofilm activity in P. acnes. Evaluation of 157 extracts from 10 fungi and 58 plants led to the identification of crude extracts from seven specie…
CARATTERIZZAZIONE MOLECOLARE DI BATTERI IN REPERTI LIGNEI SOMMERSI
2013
The focus of this study was the identification of bacterial colonies in waterlogged wood fragments from the rostrum of a excellent workmanship, that is very likely one of the wrecks attributed to Sextus Pompey fleet (36 BC) and discovered in Acqualadroni, Messina, Sicily, Italy (2008). The wood samples were analyzed by light and Scanning Electron Microscopy (SEM), in vitro culture and molecular technique (DNA base techniques). The results, focused on bacterial consortia, allowed us to reveal the presence of Pseudomonas sp., Sphingomonas sp., Xanthomonas sp. besides Marinobacter sp. and Desulforudis audaxviator. A prompt and accurate characterization of bacterial colonization represents one …
BIOREMOVAL 0F SULPHATE LAYER FROM A 15TH CENTURY POLYCHROME MARBLE ARTIFACT
2015
This case study shows the application of viable bacterial cells for removing undesired deposits from the surface of a stone polychrome bas-relief, exhibited at the Interdisciplinary Gallery of Sicily Palazzo Abatellis (Palermo). Diagnostic studies carried out during the restoration were aimed at characterizing the technique of execution and understanding the degradation processes. The results of the XRF and FTIR investigations showed that on large areas of the artifact’s surface, stratified deposits with a significant presence of sulfates were present. For bioremoval of the deposits, viable bacterial cells belonging to the species Desulfovibrio vulgaris, were utilized in gelled solution for…
Differential response of benthic microbes and meiofauna to fish-farm disturbance in coastal sediments
2001
Bacterial and meiofaunal abundance and biomass and their response to the disturbance induced by fish-farm biodeposition were investigated from March to October 1997 on a monthly basis at two stations of the Gaeta Gulf (Tyrrhenian Sea, Mediterranean Sea). The biopolymeric fraction of the organic matter was characterized by high concentrations which was similar at both fish-farming-impacted and control stations. Similarly, bacteria accounted for a small fraction of the biopolymeric organic carbon (<1%), while the contribution due to auto-fluorescent cell biomass (i.e. prokaryotic and eukaryotic cells displaying auto-fluorescence) to the total biopolymeric carbon was quantitatively negligible …
Intrinsic bioremediation potential of a chronically polluted marine coastal area.
2015
A microbiological survey of the Priolo Bay (eastern coast of Sicily, Ionian Sea), a chronically polluted marine coastal area, was carried out in order to discern its intrinsic bioremediation potential. Microbiological analysis, 16S rDNA-based DGGE fingerprinting and PLFAs analysis were performed on seawater and sediment samples from six stations on two transects. Higher diversity and variability among stations was detected by DGGE in sediment than in water samples although seawater revealed higher diversity of culturable hydrocarbon-degrading bacteria. The most polluted sediment hosted higher total bacterial diversity and higher abundance and diversity of culturable HC degraders. Alkane- an…