Search results for "bacterial"

Spatial processes driving soil microbial community assembly on a wide scale

2012

International audience; Soil houses a huge biodiversity involved in ecological services through microbial community assembly. However, processes driving soil microbial community assembly are still scarcely understood, particularly the relative importance of environmental heterogeneity regarding to dispersal limitations. This can be achieved through studying the determinism of taxa-area relationship (TAR, how community composition change with geographic distance), a fundamental relationship in ecology. Here, a biogeographical approach was applied on a wide scale to evaluate TAR for soil bacterial and fungal communities and to partition their spatial variations into environmental heterogeneit…

Do tropical soil engineer stimulate the bacterial communities link to N2O emission?

2009

National audience

Recherche d'indicateurs de la résistance des sols aux maladies d'origine tellurique

2014

Development of a new functional marker for β-triketone herbicides exposure in agricultural soils

2018

The β-triketone herbicides are maize selective herbicides that have been largely applied in replacement of atrazine, banned in Europe in 2003. Their mode of action lays on the inhibition of the p-hydroxyphenylpyruvate dioxygenase (HPPD), a key enzyme of the carotenoid biosynthesis. In recent studies, we showed that within the soil bacterial community, many microorganisms possess a functional HPPD enzyme involved in tyrosine metabolism. These “non-target organisms” harbor the target of the β-triketone herbicides and consequently may be affected in response to its exposure. Within this context, the objective of our work is to check for the interest of hppd bacterial community as a marker of e…

Use of fonctional genes to quantify denitrifiers in the environment

2006

 ; During the last decade, application of molecular methods using cultivation-independent approaches has provided new insights into the composition and structure of denitrifying communities in various environments. However, little is known about their abundance, and quantification is still performed using cultivation-based approaches, which are not only biased by the inability to cultivate of many micro-organisms but also fastidious and time-consuming. Two types of cultivation-independent approaches have recently been developed to quantify denitrifiers. The first type, which is based on the hybridization technique, comprises the use of Southern hybridization and DNA arrays. The second type,…

Dispersal potential of antibiotrophy along the manure-soil-sediment continuum of sulfamethazine-contaminated agrosystems

2021

An emerging function in the scientific literature, antibiotrophy is the bacterial capacity to metabolize antibiotics. Considered a bioremediation tool, it remains a health risk due to its association with antibiotic resistance. The first goal of this thesis was to study its dispersion within agrosystems contaminated with antibiotics during two coalescence events, i.e. manure spreading on the soil and its erosion towards the aquatic compartment. Experiments have shown the systematic invasion of the soil and sediment bacterial communities by the antibiotroph Microbacterium sp. C448 when sulfamethazine is present but not necessarily mineralized. The antibiotic would have a double function towa…

Contrasting Spatial Patterns and Ecological Attributes of Soil Bacterial Taxa across French National Territory

2017

EA SPE BIOME GENOSOL UB UB Agrosup EA SPE BIOME GENOSOL UB UB AgrosupEASPEBIOMEGENOSOLUB UB Agrosup; Even though recent studies have clarified the influence of environmental filters on bacterial community diversity, those constraining bacterial populations variations remain unclear. In consequence, our ability to understand the ecological attributes of soil bacteria and to predict microbial community response to environmental stress is therefore limited. Here, we characterized the bacterial community composition and the various bacterial taxonomic groups constituting the community across the French country, by using a 16x16 km systematic grid representing 1825 sites to precisely decipher th…

Response of soil bacterial and hppd communities to tembotrione herbicide

2022

Herbicides used in agriculture aim to prevent weed growth but are known to end up in contactwith soil microorganisms, thus defined as non-target organisms. Tembotrione, a recentlymarketed β-triketone herbicide, is known to inhibit the 4-HydroxyPhenylPyruvateDioxygenase(4-HPPD) in weeds. This enzyme is also found in numerous soil microorganisms, such as somePGPR and symbiotic bacteria, that play a key role in maintenance of ecosystem services.In this study, one of the major concerns is to assess whether tembotrione could have toxiceffects on soil microorganisms and could disturb soil microbial community dynamic andstructure. To investigate the possible impacts of this herbicide on these comm…

Detection of Escherichia coli strains producing cytotoxic necrotizing factor type two (CNF2) by enzyme-linked immunosorbent assay

1994

Sheep and rabbit antisera were produced against lysates of E. coli strain 711 (pVir). This K-12 strain carries the Vir plasmid which codes for Cytotoxic Necrotizing Factor type 2 (CNF2). Immunoglobulin G (IgG) fractions of both immune sera were subsequently purified by a two-step precipitation method. To increase the specificity for CNF2, the sheep IgG preparation was extensively adsorbed against both a sonicated extract of isogenic K-12 strain 711 and intact phenol-treated cells of vaccine strain 711 (pVir). An enzyme-linked immunosorbent assay (ELISA) was developed to detect clinical isolates of E. coli producing CNF2, using the final preparations of rabbit and sheep IgG in a double sandw…

Diversity and complexity of microbial communities from a chlor-alkali tailings dump

2015

Abstract Revegetation of the tailings dumps produced by various industrial activities is necessary to prevent dust storms and erosion and represents a great challenge for ecological restoration. Little is known about the microbial colonisation and community structure of revegetated tailings following site exploitation. Here, we report the sequencing of 16S rRNA and internal transcribed spacer (ITS) fungal RNA gene amplicons from chlor-alkali residue and from an adjacent undisturbed soil to define the composition and assembly of the rhizosphere microbial communities. After quality filtering, a total of 72,373 and 89,929 bacterial sequences and 122,618 and 111,209 fungal sequences remained fo…