Search results for "betalain"

Phytochemical Indicaxanthin Inhibits Colon Cancer Cell Growth and Affects the DNA Methylation Status by Influencing Epigenetically Modifying Enzyme E…

2015

<b><i>Background:</i></b> Recently, we have shown anti-proliferative and pro-apoptotic effects of indicaxanthin associated with epigenetic modulation of the onco-suppressor <i>p16</i><sup><i>INK4a</i></sup> in the human colon cancer cell line CACO2. In the present study, the epigenetic activity of indicaxanthin and the mechanisms involved were further investigated in other colorectal cancer cell lines. <b><i>Methods:</i></b> LOVO1, CACO2, HT29, HCT116, and DLD1 cells were used to evaluate the potential influence of consistent dietary concentrations of indicaxanthin on DNA methylation, and the epigenetic mech…

Transepithelial transport of the bioactive betalain pigments indicaxanthin and betanin across human intestinal Caco-2 cell monolayers

2012

Increased resistance to oxidation of betalain-enriched human low density lipoproteins

2003

Betalains are natural pigments recently considered as compounds with potential antioxidative properties. In this work, ex vivo plasma spiking of pure either betanin or indicaxanthin, followed by isolation of low density lipoprotein (LDL), and measurement of its resistance to copper-induced oxidation, has been used to research if these betalains can bind to LDL and prevent oxidation of LDL lipids. When pooled human plasma from 10 healthy volunteers was incubated in the presence of 25-100 microM either betanin or indicaxanthin, incorporation of both compounds in LDL was observed, with a maximum binding of 0.52 +/- 0.08, and 0.51 +/- 0.06 nmoles of indicaxanthin and betanin, respectively, per …

Antioxidant betalains from cactus pear (Opuntia ficus-indica) inhibit endothelial ICAM-1 expression.

2004

It has been suggested that some pigments would have antioxidant properties and that their presence in dietary constituents would contribute to reduce the risk of oxidative stress–correlated diseases. Among others, inflammatory response depends on redox status and may implicate oxidative stress. Vascular endothelial cells are a direct target of oxidative stress in inflammation. We have tested the impact of the free radical scavenger and antioxidant properties of betalains from the prickle pear in an in vitro model of endothelial cells. Here we show the capacity of betalains to protect endothelium from cytokine- induced redox state alteration, through ICAM-1 inhibition. KEYWORDS: endothelial …

Anti-proliferative effect of betalains from Sicilian cactus pear (Opuntia ficus indica, L. Mill.) on malignant HepG2, Huh-7, and HA22T human liver ce…

2008

Antioxidant activities of prickly pear ( Opuntia ficus indica) fruits and its betalains: betanin and indicaxanthin.

2004

Phytochemicals and phytomedicines are now an expanding research fields. A great number of active agents occurring in plants and herbs have been discovered, which is fundamental to find a rationale for the health effects of these herbs, in many cases used for centuries as traditional remedies. The knowledge of the mechanisms and molecular basis of their action is the final objective to understand the mode of action of the discovered principles. Studies on the bioactivity of cactus pear are very recent and results obtained so far, while exciting, now generate new questions. In vivo studies on healthy humans showed a strong protection of the body antioxidant system, and a marked reduction of p…

Uptake of Betanin and Indicaxanthin by Caco-2 cell monolayer.

2009

Transepithelial transport of betalainic phytochemicals in Caco-2 cell monolayers.

2010

Anti-oxidant and anti-inflammatory properties of betalains from Cactus Pear

2004

Anti-oxidant and anti-inflammatory properties of Betalains from Cactus Pear. C. Gentile1, A. Marconi1, M. A. Livrea2, G. Uzan1, P. D’Alessio1. 1 Inserm U602, Paris, France. 2 Dip. Farmacochimico, Tossicologico e Biologico, Università Palermo, Italy. Betalains of phytochemical origin, known as potent radical-scavengers in chemical and biological systems, have been tested in a model of inflammation. New phytochemicals are continuously inspected for potential pharmacological applications. The red betanin and the yellow indicaxanthin have recently been isolated and characterized from Cactus Pear. Reactive Oxygen Species may be toxic or display a regulatory character. During the inflammation rea…

Cytoprotective effects of the antioxidant phytochemical indicaxanthin in beta-thalassemia red blood cells

2006

Antioxidant phytochemicals are investigated as novel treatments for supportive therapy in beta-thalassemia. The dietary indicaxanthin was assessed for its protective effects on human beta-thalassemic RBCs submitted in vitro to oxidative haemolysis by cumene hydroperoxide. Indicaxanthin at 1.0-10 microM enhanced the resistance to haemolysis dose-dependently. In addition, it prevented lipid and haemoglobin (Hb) oxidation, and retarded vitamin E and GSH depletion. After ex vivo spiking of blood from thalassemia patients with indicaxanthin, the phytochemical was recovered in the soluble cell compartment of the RBCs. A spectrophotometric study showed that indicaxanthin can reduce perferryl-Hb ge…