Search results for "binding"

Replication-independent expression of H1˚ and H3.3 histone variants is probably regulated by different RNA-binding proteins

2012

DNA in eukaryotes is wrapped around core histones to form nucleosomes, the basic units of chromatin. The linker histones H1 bind DNA where it enters and leaves the nucleosome, thus stabilizing higher order structures. Chromatin is a dynamic complex, modulated by different processes such as DNA-methylation, post-translational modifications of histones, and incorporation of specific histone variants. Throughout rat brain development, expression of H1° and H3.3 histone variants is mainly regulated at the post-transcriptional level. These proteins are of interest for their possible involvement in the replication-independent chromatin remodelling induced by extracellular stimuli. We previously c…

Histone H1° and H3.3 RNA-binding proteins identified in the developing rat brain

2011

Heterogeneity and temporal dynamics of evolution of G1 human rotaviruses in a settled population.

2006

ABSTRACT A rotavirus sample collection from 19 consecutive years was used to investigate the heterogeneity and the dynamics of evolution of G1 rotavirus strains in a geographically defined population. Phylogenetic analysis of the VP7 gene sequences of G1P[8] human rotavirus strains showed the circulation of a heterogeneous population comprising three lineages and seven sublineages. Increases in the circulation of G1 rotaviruses were apparently associated with the introduction of novel G1 strains that exhibited multiple amino acid changes in antigenic regions involved in rotavirus neutralization compared to the strains circulating in the previous years. The emergence and/or introduction of G…

Evaluation of multivalency as an organization principle for the efficient synthesis of doubly and triply threaded amide rotaxanes

2014

Mono-, di- and trivalent pseudorotaxanes with tetralactam macrocycle hosts and axles containing diamide binding stations as the guests have been synthesised. Their threading behaviour was analyzed in detail by NMR experiments and isothermal titration calorimetry. An X-ray crystal structure of the monovalent pseudorotaxane confirms the binding motif. Double mutant cycle analysis provides the effective molarities and insight into the chelate cooperativity of multivalent binding. While the second binding event in a trivalent pseudorotaxane exhibits a slightly positive cooperativity, the third binding is nearly non-cooperative. Nevertheless, the enhanced binding affinities resulting from the mu…

Rubinstein-Taybi syndrome (CREBBP, EP300)

2011

1.2 OMIM# of the disease180849.1.3 Name of the analyzed genes or DNA/chromosome segmentsCREBBP, EP300 (E1A binding protein p300).1.4 OMIM# of the genes600140 (CREBBP), 602700 (EP300).1.5 Mutational spectrumMainly frameshift, nonsense, splice site and missense mutations. Lessfrequently large deletions (one or more exons) and rarely balancedinversions and translocations. Mutations are heterozygous, and mosaicmutations have been described. At present, more than 100 pathogenicmutations are known for the two genes together, but mutations inEP300 are much less common (only 11 so far).

Tissue-specific mosaicism in a patient with Rubinstein–Taybi syndrome and CREBBP exon 1 duplication

2019

The telomeric Cdc13-Stn1-Ten1 complex regulates RNA polymerase II transcription

2019

Advance article.

Sabella spallanzanii mucus contain a galactose-binding lectin able to agglutinate bacteria. Purification and characterization

2019

Lectins are present in almost all living organisms and are involved in several biological processes, including immune responses. In the present study, a calcium dependent galactose-binding lectin exhibiting an apparent MW of 43 kDa has been characterized and purified from the mucus of the polychaete Sabella spallanzanii by using both affinity chromatography and high-pressure liquid chromatographic methods. Its agglutinating activity towards rabbit erythrocytes was significantly modified by the addition of calcium or EDTA. The activity was optimal at temperature values comprised between 4 and 18 °C, maintain a 50% of activity between 20 and 37 °C, was significant deleted after exposure at 50…

Differential occurrence of S100A7 in breast cancer tissues: A proteomic-based investigation

2012

Purpose The present study reports for the first time a large-scale proteomic screening of the occurrence, subcellular localization and relative quantification of the S100A7 protein among a group of 100 patients, clinically grouped for the diagnosis of infiltrating ductal carcinoma (IDC). Experimental design To this purpose, the methods of differential proteomics, Western blotting, and immunohistochemistry were used. Results The identity of two isoforms of the protein was assessed by mass spectrometry and immunologically confirmed. Moreover, we proved by immunocytochemical applications the exclusive localization of the protein within the neoplastic cells. The correlation of S100A7 expression…

In Silico Design of New Dual Inhibitors of SARS-CoV-2 MPRO through Ligand- and Structure-Based Methods

2023

The viral main protease is one of the most attractive targets among all key enzymes involved in the life cycle of SARS-CoV-2. Considering its mechanism of action, both the catalytic and dimerization regions could represent crucial sites for modulating its activity. Dual-binding the SARS-CoV-2 main protease inhibitors could arrest the replication process of the virus by simultaneously preventing dimerization and proteolytic activity. To this aim, in the present work, we identified two series’ of small molecules with a significant affinity for SARS-CoV-2 MPRO, by a hybrid virtual screening protocol, combining ligand- and structure-based approaches with multivariate statistical analysis. The B…