Search results for "biofilm."

A novel intrinsically disordered outer membrane lipoprotein ofAggregatibacter actinomycetemcomitansbinds various cytokines and plays a role in biofil…

2017

Intrinsically disordered proteins (IDPs) do not have a well-defined and stable 3-dimensional fold. Some IDPs can function as either transient or permanent binders of other proteins and may interact with an array of ligands by adopting different conformations. A novel outer membrane lipoprotein, bacterial interleukin receptor I (BilRI) of the opportunistic oral pathogen Aggregatibacter actinomycetemcomitans binds a key gatekeeper proinflammatory cytokine interleukin (IL)-1b. Because the amino acid sequence of the novel lipoprotein resembles that of fibrinogen binder A of Haemophilus ducreyi, BilRI could have the potential to bind other proteins, such as host matrix proteins. However, from th…

Polimeri antibatterici contenenti carvacrolo come approccio innovativo per il controllo del biofilm

2011

Antibiofilm activity of a novel pyrrolomycin against staphylococcal biofilms of veterinary interest

2011

bbFISH-ing in the sonication fluid

2019

Abstract By 2030, the annual number of combined total hip and knee arthroplasty is estimated to reach 3.5 to 4 million in the US alone. In the context of a constant increase of the number of primary and revision total hip and knee arthroplasty, an increased risk of complication is expected. Prosthetic joint infections (PJIs) represent major cause of healthcare expenditure and morbidity. PJI still remain the most common and feared arthroplasty complication. A rapid and correct diagnosis of infection is decisive for a correct therapeutical management. In this setting, the Academic Emergency Hospital Sibiu adopted and implemented, with the beginning of September 2016, a new strategy for the di…

Discovery of a new class of sortase a transpeptidase inhibitors to tackle gram-positive pathogens: 2-(2-phenylhydrazinylidene)alkanoic acids and rela…

2016

A FRET-based random screening assay was used to generate hit compounds as sortase A inhibitors that allowed us to identify ethyl 3-oxo-2-(2-phenylhydrazinylidene)butanoate as an example of a new class of sortase A inhibitors. Other analogues were generated by changing the ethoxycarbonyl function for a carboxy, cyano or amide group, or introducing substituents in the phenyl ring of the ester and acid derivatives. The most active derivative found was 3-oxo-2-(2-(3,4dichlorophenyl)hydrazinylidene)butanoic acid (2b), showing an IC50 value of 50 µM. For a preliminary assessment of their antivirulence properties the new derivatives were tested for their antibiofilm activity. The most active compo…

Pyrrolomycins as novel agents against staphylococcal biofilms

2009

Bacterial biofilms: a challenge in the discovery of new anti-infective agents.

2010

The ability to form a biofilm, bacterial communities able to grow on surfaces and surrounded by an extracellular polymeric substance biofilm agents.Our research group focused on discovering of novel chemical agents of potential therapeutic utility in the treatment of staphylococcal biofilm-associatedinfections by screening novel compounds (synthetic or natural). In a recent study, the antistaphylococcalbiofilm activity of pyrrolomycins C,D, F1, F2a, F2b, F3 a family of halogenated pyrroles which are naturally produced byActinosporangium vitaminophylum and of the synthesized related compounds I, II, III were investigated and compared (Schillaci et al, 2010). We also focused on marine inverte…

Implicación y caracterización de Staphylococcus aureus en las poliposis nasales.

2016

La rinosinusitis crónica (RSC) es una enfermedad inflamatoria, de más de 12 semanas de duración, que afecta a la mucosa de las fosas nasales y de los senos paranasales. Se caracteriza por presentar, obstrucción o congestión nasal, y/o rinorrea; y además poder presentar uno de estos dos síntomas, dolor o sensación de presión facial, y/o pérdida total o parcial del sentido del olfato. La RSC se divide en dos grupos RSC con ausencia o RSC con presencia de poliposis nasal (PN). Además clínicamente la rinosinusitis crónica con presencia de poliposis nasal (RSCcPN) pueden subclasificarse en (a) RSCcPN eosinofílica, que es bilateral e idiopática, pudiendo presentarse de forma aislada, asociada a a…

Relationship between volume of submandibular salivary stones in vivo determined with cone-beam computer tomography and in vitro with micro-computer t…

2021

Background Successful removal of salivary stones depends on exact pretreatment information of the location, the size and shape of the stones. This study aimed to compare the volume of submandibular sialoliths determined by preoperative Cone-Beam Computer Tomography (CBCT) scans with the volume of the removed stones on micro-Computer Tomography (micro-CT) scans. Material and Methods In this study, using twenty-one submandibular sialoliths, the pretreatment volumes in-vivo measured on CBCT were compared to the volumes of removed stones determined by micro-CT scans. The volume measured on micro-CT scans served as the gold standard. Pre-operative CBCT’s and in-vitro micro-CT’s were converted in…

Thiazole Analogues of the Marine Alkaloid Nortopsentin as Inhibitors of Bacterial Biofilm Formation

2020

Anti-virulence strategy is currently considered a promising approach to overcome the global threat of the antibiotic resistance. Among different bacterial virulence factors, the biofilm formation is recognized as one of the most relevant. Considering the high and growing percentage of multi-drug resistant infections that are biofilm-mediated, new therapeutic agents capable of counteracting the formation of biofilms are urgently required. In this scenario, a new series of 18 thiazole derivatives was efficiently synthesized and evaluated for its ability to inhibit biofilm formation against the Gram-positive bacterial reference strains Staphylococcus aureus ATCC 25923 and S. aureus ATCC 6538 a…