Search results for "biokemia"
showing 5 items of 25 documents
Serum sex-hormone binding globulin and cortisol concentrations are associated with overreaching during strenuous military training
2011
The purpose was (a) to study the effect of an 8-week Finnish military basic training period (BT) on physical fitness, body composition, mood state, and serum biochemical parameters among new conscripts; (b) to determine the incidence of overreaching (OR); and (c) to evaluate whether initial levels or training responses differ between OR and noOR subjects. Fifty-seven males (19.7 ± 0.3 years) were evaluated before and during BT. Overreaching subjects had to fulfill 3 of 5 criteria: decreased aerobic physical fitness (VO2max), increased rating of perceived exertion (RPE) in 45-minute submaximal test at 70% of VO2max or sick absence from these tests, increased somatic or emotional symptoms of …
The Interconnecting Hairpin Extension "Arm": An Essential Allosteric Element of Phytochrome Activity
2023
In red-light sensing phytochromes, isomerization of the bilin chromophore triggers structural and dynamic changes across multiple domains, ultimately leading to control of the output module (OPM) activity. In between, a hairpin structure, "arm", extends from an interconnecting domain to the chromophore region. Here, by removing this protein segment in a bacteriophytochrome from Deinococcus radiodurans (DrBphP), we show that the arm is crucial for signal transduction. Crystallographic, spectroscopic, and biochemical data indicate that this variant maintains the properties of DrBphP in the resting state. Spectroscopic data also reveal that the armless systems maintain the ability to respond t…
Dietary differences between Baltic ringed seals (Phoca hispida botnica) based on stable isotope and fatty acid analyses
2014
Itämerennorppa (Phoca hispida botnica) on uhanalainen ja suojeltu laji Itämerellä, sillä hyljekannat romahtivat 1900-luvulla laajan metsästyksen ja ympäristömyrkkyjen vuoksi. Perämerellä hyljekanta on tällä hetkellä kasvussa, ja harmaahylkeiden (Halichoerus grypus) ja itämerennorppien aiheuttamat vahingot ovat kasvaneet. Hylkeiden vuoksi kalastajat ovat menettäneet saalista. Hylkeet ovat myös vahingoittaneet kaloja ja rikkoneet pyydyksiä. Eniten tuhoa hylkeet ovat aiheuttaneet siika- (Coregonus lavaretus (L.), kuha- (Sander lucioperca (L.) ja lohi- (Salmo salar L.) kalastukselle. Tutkimuksen tavoitteena oli tutkia 45 itämerennorpan ravintoa kahden biokemiallisen menetelmän avulla: vakaat is…
Exercise as stimulus for cartilage health in knee osteoarthritis
2018
Knee osteoarthritis (OA) is a leading cause of musculoskeletal pain and disability and has significant socio-economical costs globally. There is no known cure for OA. Therapeutic exercise has been shown to evoke acute positive posttreatment effects on pain and function. Even though cartilage degeneration is crucial in OA progression, there is sparse knowledge about exercise effects on osteoarthritic cartilage. The purpose of this study was to investigate the efficacy of aquatic resistance training on the estimated biochemical composition of tibiofemoral cartilage and the association between leisure time physical activity (LTPA) and tibiofemoral cartilage in postmenopausal women with mild kn…
Quantitative microscopy reveals stepwise alteration of chromatin structure during herpesvirus infection
2019
During lytic herpes simplex virus 1 (HSV-1) infection, the expansion of the viral replication compartments leads to an enrichment of the host chromatin in the peripheral nucleoplasm. We have shown previously that HSV-1 infection induces the formation of channels through the compacted peripheral chromatin. Here, we used three-dimensional confocal and expansion microscopy, soft X-ray tomography, electron microscopy, and random walk simulations to analyze the kinetics of host chromatin redistribution and capsid localization relative to their egress site at the nuclear envelope. Our data demonstrated a gradual increase in chromatin marginalization, and the kinetics of chromatin smoothening arou…