Search results for "biologia generale"

Il Microbiota delle piante, ovvero le piante hanno bisogno dei microrganismi

2017

Le relazioni tra le piante e i fattori ambientali (il clima, i nutrienti, l’acqua, la composizione del suolo, etc) sono da tempo ampiamente studiate. Resta invece ancora molto da capire sul ruolo che la componente biologica del suolo (ci sono oltre cento milioni di cellule microbiche in un grammo di suolo!) ha sullo sviluppo e la salute delle piante. Nella rizosfera soprattutto (la porzione di suolo influenzata dalla presenza di radici), gli essudati radicali favoriscono lo sviluppo di microrganismi (batteri, funghi, protozoi, virus) che interagiscono con le radici e con il suolo nell’influenzare i processi di trasformazione ed assorbimento dei nutrienti, la crescita della pianta e le infez…

Popolazioni microbiche del corpo umano. Microbioti e Uomo: un rapporto intenso tra microrganismi ed esseri umani

2011

The small protein TrpM is involved in Streptomyces coelicolor differentiation

Biodegradation of organophosphorus pesticides by soil bacteria.

2009

A number of studies in the 1980s and 1990s showed that crop-protection products, applied to drained fields, could move downwards through the soil profile and to the groundwater. Organophosphorus insecticides (OPs) are used all over the world for crop protection, for other agricultural practices such as sheep dipping and, in aquaculture, for the control of sea lice. Ops besides showing a specific neurotoxicity and have also been related to various modern diseases, including Creutzfeldt–Jakob (CJD) and the Gulf War syndrome. Although OPs are less persistent than Organoclorine pesticides (OCs), they still constitute an environmental risks thus increasing the social concern about their levels i…

Discovery of a new class of sortase a transpeptidase inhibitors to tackle gram-positive pathogens: 2-(2-phenylhydrazinylidene)alkanoic acids and rela…

2016

A FRET-based random screening assay was used to generate hit compounds as sortase A inhibitors that allowed us to identify ethyl 3-oxo-2-(2-phenylhydrazinylidene)butanoate as an example of a new class of sortase A inhibitors. Other analogues were generated by changing the ethoxycarbonyl function for a carboxy, cyano or amide group, or introducing substituents in the phenyl ring of the ester and acid derivatives. The most active derivative found was 3-oxo-2-(2-(3,4dichlorophenyl)hydrazinylidene)butanoic acid (2b), showing an IC50 value of 50 µM. For a preliminary assessment of their antivirulence properties the new derivatives were tested for their antibiofilm activity. The most active compo…

Genetic manipulation of spiramycin-producing strain Streptomyces ambofaciens ATCC 23877 by φC31 Att/Int system-based vectors results in a significant…

The genus Streptomyces produces about two-thirds of naturally occurring antibiotics and many other biologically active secondary metabolites. The genetic manipulation of Streptomycetes is often labor and time intensive due to their large genome and complex development. Much progress has been made to develop gene transfer methods useful to construct antibiotic-producing strains with improved properties. The φC31 Att/Int system is an integration system that has been widely used to produce stable recombinants in Actinomycetes and its integration site is an attB site located in a pirin-like gene (pirA) of the bacterial chromosome. In this study we demonstrate that the integration of φC31 DNA in…

Bacterial biofilms: a challenge in the discovery of new anti-infective agents.

2010

The ability to form a biofilm, bacterial communities able to grow on surfaces and surrounded by an extracellular polymeric substance biofilm agents.Our research group focused on discovering of novel chemical agents of potential therapeutic utility in the treatment of staphylococcal biofilm-associatedinfections by screening novel compounds (synthetic or natural). In a recent study, the antistaphylococcalbiofilm activity of pyrrolomycins C,D, F1, F2a, F2b, F3 a family of halogenated pyrroles which are naturally produced byActinosporangium vitaminophylum and of the synthesized related compounds I, II, III were investigated and compared (Schillaci et al, 2010). We also focused on marine inverte…

Exploring long chain n-alkane metabolism in Gordonia sp. strain SoCg

2011

Thiazole Analogues of the Marine Alkaloid Nortopsentin as Inhibitors of Bacterial Biofilm Formation

2020

Anti-virulence strategy is currently considered a promising approach to overcome the global threat of the antibiotic resistance. Among different bacterial virulence factors, the biofilm formation is recognized as one of the most relevant. Considering the high and growing percentage of multi-drug resistant infections that are biofilm-mediated, new therapeutic agents capable of counteracting the formation of biofilms are urgently required. In this scenario, a new series of 18 thiazole derivatives was efficiently synthesized and evaluated for its ability to inhibit biofilm formation against the Gram-positive bacterial reference strains Staphylococcus aureus ATCC 25923 and S. aureus ATCC 6538 a…