Search results for "biological fluid"
showing 2 items of 22 documents
The effectiveness of the RSID confirmatory test kit for human alpha amylase: the effects of environmental factors and substrate materials
2016
The investment in time and money that DNA analyses involves, provides a justification for forensic laboratories to carry out prior tests to ensure the presence of biological fluid in samples. The RSID kit has already been evaluated for forensic use, but its effectiveness on forensic casework samples is almost impossible to predict. Moreover, forensic reports presented before a court of law must justify the methods used to generate their findings. Consequently, trials to know how factors – such as the characteristics of the donor, the substrate and environmental conditions – can affect the kit’s efficacy are essential. This study evaluates some of these factors. For this aim, samples were ma…
Determination of Plasma Lipid Hydroperoxides by an NADPH/NADP + Coupled Enzyme Reaction System. Evaluation of a Method
1998
Summary: Several techniques based on different principles have been proposed to measure lipid hydroperoxides. Enzymatic methods are sensitive and can be quite specific but they are subject to interference by inhibitors and not all are stoichiometric. The present work proposes some modifications of the Heath & Tappel (Anal Biochem 1976; 7:184—91) enzymatic method of determination of lipid hydroperoxides in order to standardize and automate it and to meet the analytical criteria required for a biological assay. The proposed new protocol and the automated assay give acceptable within-run and between-run precisions, with coefficients of variation of 3.34% and 5.80%, respectively, at the usual p…