Search results for "bioquimica"
showing 9 items of 49 documents
Interactions between abscisic acid and plastidial glycolysis in Arabidopsis
2011
[EN] The phytohormone abscisic acid (ABA) controls the development of plants and plays a crucial role in their response to adverse environmental conditions like salt and water stress.1-3 Complex interactions between ABA and sugar signal transduction pathways have been shown. However, the role played by glycolysis in these interactions is not known. In the associated study,4 we investigated the interactions between plastidial glycolytic glyceraldehyde-3-phosphate dehydrogenase (GAPCp) and ABA signal transduction in Arabidopsis. We followed physiological, genetic and genomic approaches to understand the processes and mechanisms underlying the ABAglycolysis interactions. Our results indicated …
Caspase 3 Targeted Cargo Delivery in Apoptotic Cells Using Capped Mesoporous Silica Nanoparticles
2015
[EN] Excessive apoptotic cell death is at the origin of several pathologies, such as degenerative disorders, stroke or ischemia-reperfusion damage. In this context, strategies to improve inhibition of apoptosis and other types of cell death are of interest and may represent a pharmacological opportunity for the treatment of cell-death-related disorders. In this scenario new peptide-containing delivery systems (solids S1-P1and S1-P2) are described based on meso-porous silica nanoparticles (MSNs) loaded with a dye and capped with the KKGDEVDKKARDEVDK (P1) peptide that contains two repeats of the DEVD target sequence that are selectively hydrolyzed by caspase3 (C3). This enzyme plays a central…
A genetic approach reveals different modes of action of prefoldins
2021
17 p.-7 fig.
Xanthone-photosensitized detoxification of the veterinary anthelmintic fenbendazole
2013
Fenbendazole (1) is a common veterinary anthelmintic, toxic to water living microorganisms. Fluorescence quantum yields of 1 were found to be 0.11 in acetonitrile, 0.068 in methanol, 0.034 in cyclohexane, and 0.013 in water. The singlet excited state energy was ca. 96 kcal mol(-1) in all solvents. The phosphorescence spectrum of 1 in ethanol at 77 K displayed a maximum at 450 nm, leading to a triplet energy of 75 kcal mol(-1). Experimental excited state energies agree well with the results of OFT calculations at the time-dependent B3LYP/6-311+G(d,p)//B3LYP/6-31G(d) level. Laser flash photolysis (LFP) of 1 at 266 nm led to transients absorbing in the 300-700 nm range, ascribed to radical cat…
Untargeted metabolomics of rind essential oils allowed to differentiate two closely related clementine varieties
2021
[EN] Chemical characterization of clementine varieties (Citrus clementina Hort. ex Tan.) essential oils (EO) can lead to variety identification and valorization of their potential use in food and aroma industries. The goal of this study was the chemometric discrimination between two very closely related and morphologically identical clementine varieties, Clemenules (NL) and Clemenpons (PO), based on their rind EO, to identify the differential volatile organic compounds (VOCs) and to determine their antioxidant capacity. EO rind volatile profile was determined by gas chromatography coupled to mass spectrometry in Citrus fruit at different ripening stages grown two independent years in two di…
Plastidial Glycolytic Glyceraldehyde-3-Phosphate Dehydrogenase Is an Important Determinant in the Carbon and Nitrogen Metabolism of Heterotrophic Cel…
2015
This study functionally characterizes the Arabidopsis (Arabidopsis thaliana) plastidial glycolytic isoforms of glyceraldehyde-3-phosphate dehydrogenase (GAPCp) in photosynthetic and heterotrophic cells. We expressed the enzyme in gapcp double mutants (gapcp1gapcp2) under the control of photosynthetic (Rubisco small subunit RBCS2B [RBCS]) or heterotrophic (phosphate transporter PHT1.2 [PHT]) cell-specific promoters. Expression of GAPCp1 under the control of RBCS in gapcp1gapcp2 had no significant effect on the metabolite profile or growth in the aerial part (AP). GAPCp1 expression under the control of the PHT promoter clearly affected Arabidopsis development by increasing the number of later…
Acetylcholine-responsive cargo release using acetylcholinesterase-capped nanomaterials
2019
[EN] Mesoporous silica nanoparticles capped with acetylcholinesterase, through boronic ester linkages, selectively release an entrapped cargo in the presence of acetylcholine.
Pràctiques de Bioquímica
2012
El document forma part dels materials docents programats mitjançant l'ajut del Servei de Política Lingüística de la Universitat de València Document PDF amb diverses Pràctiques de Laboratori de Bioquímica General per a un Grau de Biologia, però també utilitzable en Graus afins com Bioquímica, Biomedicina i Biotecnologia. Conté les descripcions de 5 sessions pràctiques on es fan servir les tècniques bàsiques de bioquímica: valoració d’àcids febles i tampons, aïllament, purificació i caracterització de proteïnes i d’àcids nucleics, experiències amb cadena fotosintètica de cloroplasts i del metabolisme d’hidrats de carboni (fermentació i glicogen). PDF document with several Practices of Labora…
Función del gen PIR32 en la construcción y biogénesis de la pared celular de Candida albicans
2013
Candida albicans es un hongo que forma parte de la microbiota comensal de los tractos urogenital y gastrointestinal de muchos individuos, no obstante es la especie que se encuentra con mayor frecuencia como patógeno humano y por ello es la más estudiada y utilizada como modelo de investigación con respecto a otras especies; además, la incidencia de infecciones producidas por Candida se ha incrementado en los últimos años observándose paralelamente un aumento significativo de la morbilidad y la mortalidad como consecuencia de estas infecciones. La pared celular es la característica diferencial más importante entre las células eucariotas humanas y fúngicas, lo cual la convierte en diana ideal…