Search results for "biotekniikka"

Grafeenioksidin funktionalisointi

2014

Tämän pro gradu -tutkielman aiheena on grafeenioksidin kiehtova maailma. Tutkielman tavoitteena oli tehdä kattava kirjallisuuskatsaus grafeenioksidin funktionalisoinnista. Tutkielmassa käydään läpi grafeenioksidin tärkeimmät rakenteelliset piirteet, fysikaalis-kemialliset ominaisuudet, kemialliset funktionalisointimenetelmät sekä sovelluskohteet bioteknologiassa ja muualla. Ominaisuuksien tarkastelussa on kiinnitetty erityistä huomiota hapetusasteen merkitykseen sekä grafeenioksidin fluoresenssiin. Kemiallisten funktionalisointimenetelmien osiossa keskitytään lähinnä epoksidi- ja hydroksyyliryhmien funktionalisointireaktioihin. Karboksyylihapporyhmien reaktioista on annettu myös muutamia es…

Reconfigurable DNA Origami Nanocapsule for pH-Controlled Encapsulation and Display of Cargo

2019

DNA nanotechnology provides a toolbox for creating custom and precise nanostructures with nanometer-level accuracy. These nano-objects are often static by nature and serve as versatile templates for assembling various molecular components in a user-defined way. In addition to the static structures, the intrinsic programmability of DNA nanostructures allows the design of dynamic devices that can perform predefined tasks when triggered with external stimuli, such as drug delivery vehicles whose cargo display or release can be triggered with a specified physical or chemical cue in the biological environment. Here, we present a DNA origami nanocapsule that can be loaded with cargo and reversibl…

DNA recovery from Droplet Digital™ PCR emulsions using liquid nitrogen

2020

Droplet microfluidics is a technology that enables the production and manipulation of small volumes. In biosciences, the most popular application of this technology is Droplet Digital™ PCR (ddPCR™), where parallel nanoliter-scale PCR assays are used to provide a high sensitivity and specificity for DNA detection. However, the recovery of PCR products for downstream applications such as sequencing can be challenging due to the droplets' stability. Here we compared five methods for disrupting the droplets to recover DNA. We found that rapid freezing in liquid nitrogen results in a clear phase separation and recovery of up to 70% of the DNA content. Liquid nitrogen freezing can thus offer a si…

Structural characterization of site-modified nanocapsid with monodispersed gold clusters

2017

AbstractHepatitis E Virus-like particles self-assemble in to noninfectious nanocapsids that are resistant to proteolytic/acidic mucosal delivery conditions. Previously, the nanocapsid was engineered to specifically bind and enter breast cancer cells, where successful tumor targeting was demonstrated in animal models. In the present study, the nanocapsid surface was modified with a solvent-exposed cysteine to conjugate monolayer protected gold nanoclusters (AuNC). Unlike commercially available gold nanoparticles, AuNCs monodisperse in water and are composed of a discrete number of gold atoms, forming a crystalline gold core. Au102pMBA44 (Au102) was an ideal conjugate given its small 2.5 nm s…

Biotekniikan avulla tuotettujen lääkkeiden teollista valmistusta koskeva lainsäädäntö Suomessa

2004

Hiilihydraateista bioteknisesti valmistettavat merkittävät teollisuuskemikaalit

2010

Keskustelu geenimuunnelluista elintarvikkeista : sanomalehtikirjoitukset Ulrich Beckin modernisaatioteorian näkökulmasta

2008

Tutkimuksen tavoitteena oli tarkastella geenimuunnelluista elintarvikkeista käytävää sanomalehtikeskustelua. Aikaisemmissa tutkimuksissa geenimuuntelua käsittelevää keskustelua on tutkittu usein jakamalla keskustelijat kahteen vastakkaiseen leiriin, myönteisesti ja kielteisesti suhtautuviin. Näkökulma peittää alleen enemmän tai vähemmän mielipiteestään epävarmojen keskustelijoiden joukon ja vahvistaa entisestään käsitystä ongelman teknisestä luonteesta. Tässä tutkimuksessa esiin pääsevät myös rintamalinjojen väliin sijoittuvat keskustelijat, usein varovaisen myönteisesti, mutta peruskriittisesti asennoituvat kansalaiset. Tarkoituksena oli irtautua puolesta ja vastaan jaottelusta ja tarkaste…

Plasmid Viability Depends on the Ecological Setting of Hosts within a Multiplasmid Community

2022

Plasmids are extrachromosomal genetic elements, some of which disperse horizontally between different strains and species of bacteria. They are a major factor in the dissemination of virulence factors and antibiotic resistance. Understanding the ecology of plasmids has a notable anthropocentric value, and therefore, the interactions between bacterial hosts and individual plasmids have been studied in detail. However, bacterial systems often carry multiple genetically distinct plasmids, but dynamics within these multiplasmid communities have remained unstudied. Here, we set to investigate the survival of 11 mobilizable or conjugative plasmids under five different conditions where the hosts h…

Evaluation of biotechnology research utilisation in Finland

2003

Psychrophiles: A source of cold-adapted enzymes for energy efficient biotechnological industrial processes

2021

Biocatalysts are the backbone of bioprocessing industries that are going through a phase of transition with reference to the requirement of extraordinary enzymes for various biochemical processes. This transition is well reported and documented by various researchers through elucidation of different features and applications of mesophilic and thermophilic enzymes. However, there is little information available about psychrophilic enzymes and their involvement in industrial processes. Therefore, understanding the features and functions of psychrophilic enzymes could suggest some of their novel applications in various industries such as food, agriculture, chemicals, pharmaceuticals, and waste…