Search results for "blastocystis"

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Description of an improved method for Blastocystis hominis culture and axenization

1996

An improved method for Blastocystis hominis culture and axenization was developed in the present study. Stool samples were cultured in prereduced Boeck-Drbohlav NHI modified medium (with several modifications) supplemented with antibiotics (0.4% ampicillin, 0.1% streptomycin, 0.0006% amphotericin B). Axenization was performed by the combination of partial purification of B. hominis by Ficoll-metrizoic acid gradient and inoculation in fresh medium containing active antibiotics against remaining bacteria. A total of 25 strains were obtained by this procedure. The time required for axenization ranged between 3 and 5 weeks. The generation time of axenic strains ranged from 6.6 to 12.1 h (mean +…

medicine.drug_classAntibioticsBlastocystis InfectionsMicrobiologyFecesAmpicillinAmphotericin BCentrifugation Density GradientmedicineAnimalsHumansBlastocystis hominisAxenicFecesBlastocystisGeneral VeterinarybiologyGeneral Medicinebiology.organism_classificationCulture MediaInfectious DiseasesStreptomycinInsect ScienceParasitologyCell DivisionBacteriamedicine.drugParasitology Research

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