Search results for "bula"

Structural studies of adsorbed protein (betalactoglobulin) on natural clay (montmorillonite)

2014

International audience; In this work, the adsorption of a small globular protein (betalactoglobulin, BLG), on a natural montmorillonite clay (Mt) was investigated in acidic buffer (pH = 3). The combination of different characterization techniques such as zetametry, X-ray diffraction, transmission electronic microscopy, fluorescence and solid state nuclear magnetic resonance spectroscopies shed light on the interaction mechanism between the clay mineral and the proteins. For low BLG concentration, a slight increase of the interlayer spacing of the clay mineral was noticed as well as structural changes of the protein. In contrast, as the concentration of BLG increased, the adsorption led to a…

Binding of water-soluble, globular proteins to anionic model membranes

2009

Abstract The role of electrostatics is studied in the adsorption of proteins to negatively charged (phosphatidylcholine/phosphatidylglycerol, PC/PG) and neutral (PC) small unilamellar vesicles (SUVs). For model proteins the interaction is monitored vs . pH at low ionic strength. The adsorption behaviour of lysozyme, myoglobin and albumin (isoelectronic point, p I 5–11) is investigated in SUVs, along with changes of the fluorescence emission spectra of the charged proteins, via their adsorption on SUVs. Significant adsorption of the proteins to negatively charged SUVs is found only at pH values, where the number of positive charge moieties exceeds the number of negative charge moieties on th…

1996

Developing an artificial oxygen carrier for use in humans, we polymerize native haemoglobin and myoglobin, using bifunctional, amino group specific cross-linkers, to soluble, so-called hyperpolymers. These polymers, like other polymerized globular proteins, are members of a new class of macromolecues which consist of macromolecular base units. They all have, due to the mechanisms of the chemical reaction, broad distributions of molecular weights. Fractions of hyperpolymers of human haemoglobin were obtained by employing preparative gel-permeation (size-exclusion) chromatography. The calibration curve of analytical gel-permeation chromatography (GPC) for haemoglobin hyperpolymers was determi…

Temperature effects on counterion binding to spherical polyelectrolytes: the charge-discharge transition of lignosulfonate

1995

Abstract The effect of temperature on the effective charge numbers and diffusion coefficients of polyelectrolytes has not nobee dealth with in many studies. The present study concerns the temperature behavior of lignosulfonate. Lignosulfonate is a polydisperse polyelectrolyte whose molecules are compact spheres in aqueous solutions. One of its most remarkable properties is that is loses its charge in 0.1 M NaCl aqueous solution at about 40°C. In order to explain this charge-discharge transition, a theory for ion binding to spherical polyelectrolytes based on the relative population of two hydration states of the charged groups is presented. The water molecules adjacent to the charged groups…

Demixing and polymerization in systems of anisotropic globular particles: A molecular dynamics simulation study

1991

We report a first set of results of Molecular Dynamics simulations of phase separation in a two-dimensional system of identical particles bearing attachment sites at their surface. Morphology of regions of aggregation so obtained appear similar to images of biologically self-assembled structures. Also, they evidence the relevance of the region of thermodynamic instability and of the presence and range of interaction of specific attachment sites. Particles of our system are apt to represent indifferently, though grossly, globular proteins or rigid cells, bearing specific and fixed adhesion sites at their surface. Results illustrate some basic aspects of the origin of the morphology of extend…

HACANCOi : a new Hα-detected experiment for backbone resonance assignment of intrinsically disordered proteins

2020

AbstractUnidirectional coherence transfer is highly efficient in intrinsically disordered proteins (IDPs). Their elevated ps-ns timescale dynamics ensures long transverse (T2) relaxation times allowing sophisticated coherence transfer pathway selection in comparison to folded proteins. 1Hα-detection ensures non-susceptibility to chemical exchange with the solvent and enables chemical shift assignment of consecutive proline residues, typically abundant in IDPs. However, many IDPs undergo a disorder-to-order transition upon interaction with their target protein, which leads to the loss of the favorable relaxation properties. Long coherence transfer routes now result in prohibitively large dec…

Jaunākā pirmsskolas vecuma bērnu vārdu krājuma bagātināšana didaktiskajās rotaļās

2018

Bakalaura darba tēma – “Jaunākā pirmsskolas vecuma bērnu vārdu krājuma bagātināšana didaktiskajās rotaļās”. Bakalaura darba autore: Indra Tišanova. Izvēlētās tēmas aktualitāte pamatojama ar to, ka didaktiskās rotaļas pirmsskolas vecuma bērniem rada patīkamas izjūtas, veicina sadarbības un citas nepieciešamās prasmes, tādēļ svarīgi izpētīt, kā ar rotaļu palīdzību var bagātināt bērnu vārdu krājumu. Pētījuma mērķis – teorētiski un praktiski izzināt vārdu krājuma bagātināšanas iespējas didaktiskajās rotaļās jaunākā pirmsskolas vecuma bērniem. Pētījums sastāv no divām daļām – teorētiskā un empīriskā pētījuma. Teorētiskajā daļā pētīti un izzināti latviešu un ārvalstu pedagoģijas un psiholoģijas p…

Análisis de la correlación entre la exploración física y los hallazgos en la resonancia magnética en pacientes con disfunción de la articulación temp…

2021

Objetivos: evaluación de la utilidad de la exploración física (EF) para el diagnóstico de la disfunción temporo-mandibular (DTM) en paciente con síntomas de trastornos temporo-mandibulares (TTM). Para ello pretendemos analizar la precisión (sensibilidad, especificidad, valor predictivo positivo y valor predictivo negativo) de la EF frente a la resonancia magnética (RM). Objetivos secundarios: crear un protocolo diagnóstico que permita predecir los resultados de la RM y validar este nuevo test propuesto frente a la base de datos recogida. Material y métodos: Para hacer frente a los objetivos propuestos se llevó a cabo un estudio observacional prospectivo transversal. Se recogieron de forma c…

Cirugía ambulatoria: estudio de la morbilidad tardía

2022

La cirugía ambulatoria es una actividad rutinaria de la mayoría de los hospitales de nuestro país. Su implantación y desarrollo en los últimos años ha supuesto una solución para los grandes retos de la asistencia sanitaria actual, permitiendo reducir considerablemente la lista de espera quirúrgica bajo criterios de calidad, eficiencia y seguridad. Uno de los aspectos con mayor oportunidad de mejora es el control postoperatorio domiciliario en la fase tardía de la recuperación, especialmente más allá de las 24 horas tras la intervención. El método más utilizado actualmente en España para la evaluación del estado domiciliario es la entrevista telefónica estandarizada. Esta herramienta es útil…

Oromandibular dystonia: A dental approach

2010

Oromandibular dystonia consists of prolonged spasms of contraction of the muscles of the mouth and jaw. Primary idiopathic forms and secondary forms exist. Secondary dystonia develops due to environmental factors; some cases of cranial dystonia after dental procedure have been reported, but the causal relationship between these procedures and dystonia remains unclear. Traumatic situations in the mouth, such as poor aligned dentures or multiple teeth extractions may cause an impairment of proprioception of the oral cavity, leading to subsequent development of dystonia. The clinical characteristics of oromandibular dystonia are classified according to the affected muscles. The muscles involve…