Search results for "c-AMP"

showing 2 items of 12 documents

The phototransduction cascade in the isolated chick pineal gland revisited.

2003

It is well established that the isolated chick pineal gland is directly light sensitive and that melatonin synthesis of the gland can be inhibited by exposing the gland to light during scotophase. Since not all the steps of the phototransduction cascade have been clarified to the same extent as in the retina, we have treated isolated chick pineal glands with 90 min of light during scotophase and with drugs that affect key-components of vertebrate phototransduction, i.e., cyclic guanosine monophosphate (cGMP) phosphodiesterase 6 (PDE6), cGMP levels and cGMP-gated calcium channels. The endpoint measured was the activity of the rate-limiting enzyme of melatonin synthesis, arylalkylamine N-acet…

medicine.medical_specialtyLight Signal TransductionArylamine N-AcetyltransferasePhosphodiesterase 3BiologyNitric OxidePineal GlandRetinachemistry.chemical_compoundPineal glandOrgan Culture TechniquesInternal medicinemedicineCyclic AMPAnimalsCyclic adenosine monophosphateNitric Oxide DonorsEnzyme InhibitorsMolecular BiologyCyclic guanosine monophosphateCyclic GMPMelatoninCyclic Nucleotide Phosphodiesterases Type 6Phosphoric Diester HydrolasesGeneral NeurosciencePhosphodiesteraseNatriuretic Peptide C-TypeCyclic Nucleotide Phosphodiesterases Type 3Circadian RhythmCalcium Channel Agonistsmedicine.anatomical_structureEndocrinologychemistry3'5'-Cyclic-AMP PhosphodiesterasesNeurology (clinical)PDE10ACalcium ChannelsZaprinastChickensPhotic StimulationDevelopmental BiologyEndocrine glandBrain research
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Histamine up-regulates phosphodiesterase 4 activity and reduces prostaglandin E2-inhibitory effects in human neutrophils.

2000

Objective: To investigate whether histamine produces up-regulation of phosphodiesterase (PDE) activity with functional consequences in human peripheral blood neutrophils.¶Methods: PDE activity was studied by a radioisotopic method following anion-exchange chromatography. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used for detection of mRNA transcripts of PDE4 subtypes. Cyclic AMP (cAMP) levels were measured by enzyme-immunoassay, and superoxide generation by cytochrome c reduction.¶Treatment: Neutrophils were incubated for 4 h with histamine (1 μM).¶Results: PDE4 was the only isoenzyme activity increased in treated neutrophils. Kinetic analysis showed a ∼1.5-fold increase …

medicine.medical_specialtyTranscription GeneticNeutrophilsImmunologyHeterologousBiologyDinoprostoneNeutrophil Activationchemistry.chemical_compoundPDE4BSuperoxidesInternal medicinemedicineCyclic AMPHumansProtein IsoformsRNA MessengerProstaglandin E2PharmacologyMessenger RNASuperoxideCytochrome cZymosanPhosphodiesteraseOpsonin ProteinsMolecular biologyCyclic Nucleotide Phosphodiesterases Type 4KineticsEndocrinologychemistry3'5'-Cyclic-AMP Phosphodiesterasesbiology.proteinHistaminemedicine.drugHistamineInflammation research : official journal of the European Histamine Research Society ... [et al.]
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