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showing 10 items of 2320 documents
Genetically engineered V79 Chinese hamster cells metabolically activate the cytostatic drugs cyclophosphamide and ifosfamide.
1990
V79 cells, genetically engineered to express active cytochromes P450IIB1 and P450IA1, were used to study the cytotoxicity and mutagenicity of cyclophosphamide and ifosfamide. Cyclophosphamide, tested up to a concentration of 2 mM, was not cytotoxic in V79 nor in the P450IA1-expressing V79-derived cell line XEM2. Pronounced cytotoxicity was, however, observed in the P450IIB1-expressing V79-derived cell line SD1. Induction of gene mutations (acquisition of 6-thioguanine resistance) was observed in SD1 cells as well, but the effects were weak. Ifosfamide was inactive in V79 cells, but was cytotoxic in SD1 cells. Ifosfamide mustard, an active metabolite of ifosfamide, was equally cytotoxic and …
1H, 13C and 15N NMR chemical shift assignments of cAMP-regulated phosphoprotein-19 and -16 (ARPP-19 and ARPP-16)
2020
Protein Phosphatase 2A, PP2A, the principal Serine/threonine phosphatase, has major roles in broad range of signaling pathways that include regulation of cell cycle, cell proliferation and neuronal signaling. The loss of function of PP2A is linked with many human diseases, like cancer and neurodegenerative disorders. Protein phosphatase 2A (PP2A) functions as tumor suppressor and its tumor suppressor activity is inhibited by the overexpression of PP2A inhibitor proteins in most of the cancers. ARPP-19/ARPP-16 has been identified as one of the potential PP2A inhibitor proteins. Here, we report the resonance assignment of backbone 1H, 13C and 15N atoms of human ARPP-19 and ARPP-16 proteins. T…
Ethically minded consumer behavior, retailers’ commitment to sustainable development, and store equity in hypermarkets
2020
The present paper analyzed the influence of ethically minded consumer behavior on retailer&rsquo
Les exercices gradués dans la méthode du Grammalessico francese a uso degl’italiani d’Arminio Wurmbrand Bianchi
2020
L’article se propose d’analyser les exercices du manuel d’Arminio Wurmbrand Bianchi, Grammalessico francese a uso degl’italiani, qui ont une forme et une structure que l’on retrouve dans de nombreux manuels s’inspirant des méthodes dites « synthétiques ». Wurmbrand, en effet, introduit dans son manuel une nouveauté pour pourvoir au caractère incomplet de la méthode synthétique : il propose, ainsi, avant chaque exercice, la traduction des seuls termes « radicalement dissemblables » des deux langues « similaires à l’origine » qui caractérisent la langue française et la langue italienne. Le but est de fournir aux élèves la maîtrise lexicale de la langue française avec une attention toute parti…
Estudio funcional del receptor de glucocorticoides en desarrollo y reparación epitelial
2013
Tesis doctoral; 181 páginas, ilustraciones. Este estudio se ha desarrollado en el Instituto de Biomedicina de Valencia (IBV-CSIC), bajo la dirección de la Dra. Paloma Pérez Sánchez
El cuerpo materno en la red: entre el orden de lo ?deseable? y el de lo real
2019
El estilo de vida fitness se realiza en, y a través de, un entramado subjetivo y de poder en el que circulan y se ensamblan dispositivos empresariales, terapéuticos, sanitarios y de espectacularización de los cuerpos, tendientes a inscribir la vida de la población en las tramas conflictivas de un capitalismo heterosexista, neoliberal e informacional. En dicho marco, la temporalidad, metamorfosis y plasticidad del cuerpo materno irrumpe como problema y objeto de intervención de tecnologías diversas orientadas a ajustar su tamaño, forma y devenir a los parámetros de la vida activa y saludable. En escenarios digitales, dicha tensión se materializa, por un lado, a través de dispositivos visuale…
Role of dioxygenase α-DOX2 and SA in basal response and in hexanoic acid-induced resistance of tomato (Solanum lycopersicum) plants against Botrytis …
2015
Resistance of tomato (Solanum Lycopersicum) to the fungal pathogen Botrytis cinerea requires complex interplay between hormonal signalling. In this study, we explored the involvement of new oxylipins in the tomato basal and induced response to this necrotroph through the functional analysis of the tomato α-dioxygenase2 (α-DOX2)-deficient mutant divaricata. We also investigated the role of SA in the defence response against this necrotrophic fungus using SA-deficient tomato nahG plants. The plants lacking dioxigenase α-DOX2, which catalyses oxylipins production from fatty acids, were more susceptible to Botrytis, and hexanoic acid-induced resistance (Hx-IR) was impaired; hence α-DOX2 is requ…
Inorganic phosphate is a trigger factor for Microbispora sp. ATCC-PTA-5024 growth and NAI-107 production
2014
Background NAI-107, produced by the actinomycete Microbispora sp. ATCC-PTA-5024, is a promising lantibiotic active against Gram-positive bacteria and currently in late preclinical-phase. Lantibiotics (lanthionine-containing antibiotics) are ribosomally synthesized and post-translationally modified peptides (RiPPs), encoded by structural genes as precursor peptides. The biosynthesis of biologically active compounds is developmentally controlled and it depends upon a variety of environmental stimuli and conditions. Inorganic phosphate (Pi) usually negatively regulates biologically-active molecule production in Actinomycetes, while it has been reported to have a positive control on lantibiotic…
Absence of the endo-beta-1,4-glucanases Cel1 and Cel2 reduces susceptibility to Botrytis cinerea in tomato.
2007
Cel1 and Cel2 are members of the tomato (Solanum lycopersicum Mill) endo-beta-1,4-glucanase (EGase) family that may play a role in fruit ripening and organ abscission. This work demonstrates that Cel1 protein is present in other vegetative tissues and accumulates during leaf development. We recently reported the downregulation of both the Cel1 mRNA and protein upon fungal infection, suggesting the involvement of EGases in plant-pathogen interactions. This hypothesis was confirmed by assessing the resistance to Botrytis cinerea infection of transgenic plants expressing both genes in an antisense orientation (Anti-Cel1, Anti-Cel2 and Anti-Cel1-Cel2). The Anti-Cel1-Cel2 plants showed enhanced …
In vitroplant regeneration and gene transfer in the wild tomatoLycopersicon cheesmanii
2001
SummaryThis paper reports on protocols for in vitro plant regeneration, gene transfer and on shoot salt tolerance from two accessions of L. cheesmanii (Riley). Shoot organogenesis was achieved on media with zeatin or BA/IAA combinations. The two accessions tested, LA1401 and LA530, showed differences in both their aptitude to regenerate and their salt tolerance in vitro. The accession LA530 had a higher organogenic potential than LA1401 (97% vs. 80% of organogenic explants). In contrast, none of the LA530 isolated shoots rooted when cultured in full strength MS-modified medium supplemented with 200.mM NaCl while 93% of shoots from LA1401 formed roots. Finally, Agrobacterium-mediated transfo…