Search results for "cannabinoids"
showing 10 items of 169 documents
Emerging Immnunohistochemical evidence for Direct Peripheral Control of Endocannabinoids on the Gastrointestinal Tract and Pancreas of Obese (fa/fa)a…
2012
This research has the objective to investigate immunohistochemical expression of CB1 receptor and its probable changes in Gastroenteropancreatic system (GEP) of obese and lean Zucker rats and understand the endocannabinoid pathophysiological implications in the obesity. Male obese (fa/fa) and lean Zucker rats 6 weeks old were obtained from Harlan Italy Srl; the rats were sacrificed at 8, 12 and 16 weeks old. Normal rats also were sacrificed. Specimens of stomach, jejunum-ileum and pancreas were fixed in Bouin’s mixture and embedded in paraffin; obtained sections were processed with anti-CB1 (Biosource Europe SA) by Streptavidin-Biotin-Complex Method. The findings show that CB1 receptor is e…
In vitro contamination of hair by marijuana smoke.
2004
Abstract Background: The deposition of cannabinoids on/into hair from environmental smoke can be considered as a potential source of drug findings in hair. We studied external uptake of cannabinoids from marijuana smoke, investigating possible influencing factors on drug uptake and the efficiency of decontamination procedures. Methods: Strands of a natural hair sample were moistened with water, greased with sebum or sebum/sweat, or bleached or permed. Treated and untreated samples were exposed to marijuana smoke for 60 min. Aliquots of each hair strand were either kept unwashed or were washed with methanol, dichloromethane, or 5 g/L dodecyl sulfate in water. Cannabinoid concentrations in un…
ROLE OF NITRIC OXIDE/cGMP PATHWAY IN CANNABINOID MODULATION OF PAROXYSMAL PHENOMENA
Detectability of cannabinoids in the serum samples of cannabis users: Indicators of recent cannabis use? A follow‐up study
2021
Forensic toxicologists are frequently required to predict the time of last cannabis consumption. Several studies suggested the utility of minor cannabinoids as indicators of recent cannabis use. Because several factors influence blood cannabinoid concentrations, the interpretation of serum cannabinoid concentrations remains challenging. To assess the informative value of serum cannabinoid levels in cannabis users (in total N = 117 patients, including 56 patients who stated an exact time of last cannabis use within 24 h before blood sampling), the detectability of cannabinoids, namely delta-9-tetrahydrocannabinol (delta-9-THC), 11-hydroxy-delta-9-THC, 11-nor-9-carboxy-delta-9-THC, cannabichr…
Comparing illicit drug use in 19 European cities through sewage analysis
2012
Abstract: The analysis of sewage for urinary biomarkers of illicit drugs is a promising and complementary approach for estimating the use of these substances in the general population. For the first time, this approach was simultaneously applied in 19 European cities, making it possible to directly compare illicit drug loads in Europe over a 1-week period. An inter-laboratory comparison study was performed to evaluate the analytical performance of the participating laboratories. Raw 24-hour composite sewage samples were collected from 19 European cities during a single week in March 2011 and analyzed for the urinary biomarkers of cocaine, amphetamine, ecstasy, methamphetamine and cannabis u…
COINVOLGIMENTO DELLO STRESS DEL RETICOLO E DEL PROCESSO AUTOFAGICO NELL’APOPTOSI INDOTTA DAL CANNABINOIDE SINTETICO WIN IN CELLULE DI EPATOMA UMANO I…
2009
Studi da noi condotti precedentemente hanno dimostrato la capacità del cannabinoide sintetico WIN di indurre apoptosi in cellule di epatocarcinoma umano HepG2 attraverso un meccanismo, dipendente dal fattore trascrizionale PPARg, che prevede riduzione dei livelli di alcuni fattori di sopravvivenza e attivazione di fattori pro-apoptotici della famiglia Bcl-2 (M. Giuliano et al. Biochimie. 2009). Recentemente è, inoltre, emerso che in cellule di glioma i cannabinoidi possono stimolare l’apoptosi attraverso induzione di stress del reticolo endoplasmatico seguito da autofagia. Scopo L'obiettivo del presente studio è stato quello di valutare il coinvolgimento dell’autofagia nel percorso di morte…
A comparison between the role of SPARC in osteogenic differentiation of mesenchymal stem cells and in WIN/TRAIL-induced apoptosis in osteosarcoma cel…
2012
The cannabinoid WIN induces DR5 receptor expression in hepatoma cells sensitizing them to TRAIL signal
2009
Cannabinoid-‐mediated modulation of hippocampal hyperexcitability: focus on the interplay with nitrergic system in different rat models of temporal l…
2015
Temporal lobe epilepsy (TLE) is the most common type of partial complex seizure in adulthood [1]. Within the framework of hyperexcitability, growing interest has risen on the impact of cannabinoids on the control of paroxysmal phenomena [2], despite their reputation as psychotropic substances with addictive properties [3; 4]. In this regard, it was reported that the on-demand production of endocannabinoids from over-activated postsynaptic cells inhibits neurotransmitter release, hence protecting against excitotoxicity in the hippocampus [5; 6]. Nonetheless, the potential anticonvulsant action of cannabinoids has not been fully addressed. Indeed, CB-mediated effects in animal models are attr…