Search results for "carbon-11"

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Improved radiosynthesis and preliminary in vivo evaluation of the 11C-labeled tetrazine [11C]AE-1 for pretargeted PET imaging

2019

Pretargeted nuclear imaging based on the ligation between tetrazines and nano-sized targeting agents functionalized with trans-cyclooctene (TCO) has recently been shown to improve both imaging contrast and dosimetry in nuclear imaging of nanomedicines. Herein, we describe the improved radiosynthesis of a 11 C-labeled tetrazine ([ 11 C]AE-1) and its preliminary evaluation in both mice and pigs. Pretargeted imaging in mice was carried out using both a new TCO-functionalized polyglutamic acid and a previously reported TCO-functionalized bisphosphonate system as targeting agents. Unfortunately, pretargeted imaging was not successful using these targeting agents in pair with [ 11 C]AE-1. Howeve…

Nuclear imagingClinical BiochemistryTetrazinePET imagingPharmaceutical ScienceCarbon-1101 natural sciencesBiochemistryTetrazinechemistry.chemical_compoundIn vivoTrans-cycloocteneDrug DiscoveryMolecular BiologyPretargetingPretargeting010405 organic chemistryChemistryOrganic ChemistryRadiosynthesisPet imaging3. Good health0104 chemical sciences010404 medicinal & biomolecular chemistryMolecular MedicineBiomedical engineeringBioorganic & Medicinal Chemistry Letters
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Defense Priming in Nicotiana tabacum Accelerates and Amplifies ‘New’ C/N Fluxes in Key Amino Acid Biosynthetic Pathways

2020

: In the struggle to survive herbivory by leaf-feeding insects, plants employ multiple strategies to defend themselves. One mechanism by which plants increase resistance is by intensifying their responsiveness in the production of certain defense agents to create a rapid response. Known as defense priming, this action can accelerate and amplify responses of metabolic pathways, providing plants with long-lasting resistance, especially when faced with waves of attack. In the work presented, short-lived radiotracers of carbon administered as 11CO2 and nitrogen administered as 13NH3 were applied in Nicotiana tabacum, to examine the temporal changes in &lsquo

0106 biological sciences0301 basic medicineNicotiana tabacumamino acid metabolismPlant Science01 natural sciencesplant insect herbivorySerine03 medical and health scienceschemistry.chemical_compoundBiosynthesislcsh:Botanynitrogen-13Shikimate pathwaycarbon-11Secondary metabolismEcology Evolution Behavior and SystematicsX-ray fluorescence imagingchemistry.chemical_classificationEcologybiologydefense primingJasmonic acidfungifood and beveragesbiology.organism_classificationlcsh:QK1-989Amino acidMetabolic pathway030104 developmental biologychemistryBiochemistryisotope ratio analysis010606 plant biology & botanyPlants
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