Search results for "carbonyl"

Active paraplegics are protected against exercise-induced oxidative damage through the induction of antioxidant enzymes

2016

Exercise improves functional capacity in spinal cord injury (SCI). However, exhaustive exercise, especially when sporadic, is linked to the production of reactive oxygen species that may have a detrimental effect on SCI. We aimed to study the effect of a single bout of exhaustive exercise on systemic oxidative stress parameters and on the expression of antioxidant enzymes in individuals with paraplegia. The study was conducted in the Physical Therapy department and the Physical Education and Sports department of the University of Valencia. Sixteen paraplegic subjects were submitted to a graded exercise test (GET) until volitional exhaustion. They were divided into active or non-active group…

Oxidative status in nondiabetic middle-aged subjects with metabolic syndrome: preliminary data.

2013

Elevated protein carbonyl and HIF-1α levels in eyes with proliferative diabetic retinopathy.

2013

Purpose: To evaluate the role of protein carbonyls and hypoxia inducible factor-1a (HIF-1a) in diabetic eyes with proliferative diabetic retinopathy (PDR). Methods: Prospective consecutive controlled observational study was performed. Vitreous samples were collected at the start of the 3-ppp vitrectomy. Protein carbonylation analysis was performed by Western blotting with antibody against 2,4-Dinitrophenol (anti-DNP), following derivatization of protein carbonyls with 2,4 Dinitrophenylhydrazine (DNHP). Protein carbonylation was quantified by scanning densitometry analysis and relativized to the total amount of protein into the ponceau staining of membranes. Vitreous HIF-1 a was determined w…

Altered oxidative stress in overtrained athletes

2010

The purpose of the present study was to examine the relationship between oxidative stress and overtraining syndrome. Indicators of oxidative stress (plasma protein carbonyls, nitrotyrosine, and malondialdehyde) and antioxidant status (oxygen radical absorbance capacity) were measured in severely overtrained (two women, five men) and control athletes (five women, five men). Samples were collected from both groups at baseline (i.e. in the overtraining state of overtrained athletes) and after 6 months of recovery, both at rest and immediately after an exercise test to volitional exhaustion. At baseline, overtrained athletes had higher plasma protein carbonyls at rest than controls (mean differ…

Effects of the association of aging and obesity on lipids, lipoproteins and oxidative stress biomarkers: a comparison of older with young men.

2011

In this study, plasma lipids, lipoproteins and markers of oxidant/antioxidant status were investigated in young (n = 45) and older (n = 40) obese men and compared to those in young (n = 65) and older (n = 55) normal weight controls. The purpose was to determine whether obesity exacerbates or not lipid, lipoprotein abnormalities and oxidative stress in older men. Our findings showed that all obese patients had increased plasma triglyceride, cholesterol, LDL-cholesterol, -triglyceride and HDL-triglyceride levels concentrations compared to controls (P < 0.01). However, the younger obese men had relatively larger and accentuated changes in plasma lipids and lipoproteins than the older patients.…

Protein oxidation in mild essential hypertension

2012

An intensified oxidative stress has been associated with aging and many disease, including essential hypertension (EH) [6, 11, 16]. In EH an increased level of reactive oxygen and nitrogen species (RONS) may impair the bioavailability of nitric oxide (NO), by inducing its accelerated transformation in peroxynitrite [4]. Beside this, many other pathophysiological processes in EH may be influenced by RONS, considering their harmful effects on the structural and functional properties of lipids, proteins and nucleic acids. Some points need to be further investigated, such as the relationship between oxidative stress and the degree of hypertension, and identification of the most useful marker of…

Peripheral Oxidative Stress Markers in Patients with Bipolar Disorder during Euthymia and in Siblings.

2020

Aims:Oxidative stress is increased during the acute phases of bipolar disorder (BD). Our aim here was to analyze oxidative stress biomarkers in patients with BD during euthymia and their siblings.Method:A cross-sectional study was performed in euthymic patients with BD-I (n=48), unaffected siblings (n=23) and genetically unrelated healthy controls (n=21). Protein carbonyl content (PCC), total antioxidant capacity (TRAP), lipid peroxidation (TBARS) and uric acid were measured as biomarkers of oxidative stress in blood.Results:The antioxidant capacity (TRAP) was lower (p&lt;0.001) in patients with BD compared to their siblings and controls, whereas no differences were observed in PCC, TBARS o…

Behaviour of carbonyl groups in several clinical conditions: Analysis of our survey

2019

Protein carbonylation is a marker of oxidative protein damage, that is likely involved in the pathogenesis of several diseases. The aim of this study was to evaluate the protein carbonyl (PC) groups in different clinical conditions. It included different groups of subjects: 81 trained subjects; 23 subjects with mild essential hypertension; 31 middle-aged subjects with metabolic syndrome (MS); 106 subjects with MS not selected for age (subdivided into two subgroups, with and without diabetes mellitus); 91 obese adults subdivided in two subgroups (BMI 30-35 Kg/m2 and BMI &gt; 35 kg/m2); 48 subjects with obstructive sleep apnea syndrome (OSAS) subdivided in accordance with the apnea/hypopnea i…

Protein carbonyl groups in trained subjects before and after a cardiopulmonary test

2013

Physical exercise influences the body's oxidative status. The modifications can involve lipids, proteins and nucleic acids, and different effects seem to be induced by regular and acute exercise respectively. We examined protein oxidation, expressed as concentration of protein carbonyl groups (PC), in trained subjects before (time 0), 10 min (time 1) and 24 hours (time 2) after a cardiopulmonary test performed on a cycloergometer. We enrolled 38 trained subjects (26 men and 12 women), subdivided in two groups (A1 and B1) of 19 subjects each, according to the median value of VO2max, and in two groups (A2 and B2) of 19 subjects each, according to the median value of PC at baseline. PC concent…

Protein carbonyl groups in trained subjects.

2012

The purpose of this research was to evaluate the plasma protein carbonyl groups (PC) in 81 trained subjects (TS) who practiced regular, non professional physical activity. They were divided into three groups according to the type of sport they practiced (endurance, mixed or power). On fasting venous blood we examined the PC groups employing an enzyme-linked immunosorbent assay (ELISA) kit, in which 2,4-dinitrophenyl-hydrazine reacts with the PC forming a stable hydrazone product. In the whole group of TS a significant decrease in PC was present, in comparison with sedentary controls (SC). Dividing TS into groups, we observed a decreased PC concentration in those practicing endurance and mix…