Search results for "case-control"

showing 10 items of 1685 documents

Tumorigenic and metastatic activity of human thyroid cancer stem cells

2010

Abstract Thyroid carcinoma is the most common endocrine malignancy and the first cause of death among endocrine cancers. We show that the tumorigenic capacity in thyroid cancer is confined in a small subpopulation of stem-like cells with high aldehyde dehydrogenase (ALDHhigh) activity and unlimited replication potential. ALDHhigh cells can be expanded indefinitely in vitro as tumor spheres, which retain the tumorigenic potential upon delivery in immunocompromised mice. Orthotopic injection of minute numbers of thyroid cancer stem cells recapitulates the behavior of the parental tumor, including the aggressive metastatic features of undifferentiated thyroid carcinomas, which are sustained by…

OncologyMaleCancer ResearchLung NeoplasmsPapillaryNudeMessengerThyroid GlandFluorescent Antibody TechniqueTYROSINE KINASEMice SCIDCell TransformationImmunoenzyme TechniquesMiceMice Inbred NODCell MovementAdenocarcinoma FollicularThyroid cancerRADIOACTIVE IODINETumor Stem Cell AssayEPITHELIAL-MESENCHYMAL TRANSITION; ALDEHYDE DEHYDROGENASE-ACTIVITY; ACUTE MYELOID-LEUKEMIA; RADIOACTIVE IODINE; TYROSINE KINASE; LUNG-CANCER; CARCINOMA; RECEPTOR; GROWTH; DIFFERENTIATIONBlottingReverse Transcriptase Polymerase Chain ReactionThyroidMiddle AgedProto-Oncogene Proteins c-metFlow CytometryEPITHELIAL-MESENCHYMAL TRANSITIONmedicine.anatomical_structureCell Transformation NeoplasticDIFFERENTIATIONOncologyNeoplastic Stem CellsAdenocarcinomaGROWTHFemaleStem cellWesternAdultmedicine.medical_specialtyBlotting WesternMice NudeACUTE MYELOID-LEUKEMIABiologyAdenocarcinomaSCIDALDEHYDE DEHYDROGENASE-ACTIVITYThyroid carcinomaYoung AdultLUNG-CANCERAdenocarcinoma Follicular; Adult; Aged; Aldehyde Dehydrogenase; Animals; Blotting Western; Carcinoma; Carcinoma Papillary; Case-Control Studies; Cell Adhesion; Cell Movement; Cell Proliferation; Cell Transformation Neoplastic; Female; Flow Cytometry; Fluorescent Antibody Technique; Humans; Immunoenzyme Techniques; Lung Neoplasms; Male; Mice; Mice Inbred NOD; Mice Nude; Mice SCID; Middle Aged; Neoplasm Invasiveness; Neoplastic Stem Cells; Proto-Oncogene Proteins c-akt; Proto-Oncogene Proteins c-met; RNA Messenger; Reverse Transcriptase Polymerase Chain Reaction; Thyroid Gland; Thyroid Neoplasms; Tumor Stem Cell Assay; Xenograft Model Antitumor Assays; Young Adult; Cancer Research; OncologyCancer stem cellSettore MED/04 - PATOLOGIA GENERALEInternal medicinemedicineCell AdhesionAnimalsHumansNeoplasm InvasivenessRNA MessengerThyroid NeoplasmsALDH Human Thyroid Cancer Stem CellsAgedCell ProliferationNeoplasticRECEPTORCarcinomaFollicularTumor Stem Cell AssayCancerAldehyde Dehydrogenasemedicine.diseaseXenograft Model Antitumor AssaysCarcinoma PapillaryCase-Control StudiesInbred NODRNAProto-Oncogene Proteins c-akt
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Exome and immune cell score analyses reveal great variation within synchronous primary colorectal cancers

2019

BACKGROUND: Approximately 4% of colorectal cancer (CRC) patients have at least two simultaneous cancers in the colon. Due to the shared environment, these synchronous CRCs (SCRCs) provide a unique setting to study colorectal carcinogenesis. Understanding whether these tumours are genetically similar or distinct is essential when designing therapeutic approaches. METHODS: We performed exome sequencing of 47 primary cancers and corresponding normal samples from 23 patients. Additionally, we carried out a comprehensive mutational signature analysis to assess whether tumours had undergone similar mutational processes and the first immune cell score analysis (IS) of SCRC to analyse the interplay…

OncologyMaleCancer ResearchPROGNOSISCD3 ComplexColorectal cancerFEATURESmedicine.medical_treatmentDNA Mutational AnalysisCD8-Positive T-Lymphocytesmedicine.disease_causeTargeted therapyNeoplasms Multiple Primary0302 clinical medicineMUTATIONAL PROCESSESExomeLymphocytesExomeCancer geneticsExome sequencingAged 80 and overMutationMETHYLATIONMiddle Aged3. Good healthOncology030220 oncology & carcinogenesisDNA mismatch repairFemaleMicrosatellite InstabilityKRASColorectal Neoplasmsmedicine.medical_specialtyCARCINOMACD8 Antigens3122 Cancerscancer geneticscolorectal cancersuolistosyövätBiologyArticle03 medical and health sciencesCOLONInternal medicineKRASmedicineHumansSIGNATURESIMMUNOSCOREAgedDNA-analyysiMicrosatellite instabilitymedicine.diseaseColorectal cancerCase-Control StudiesMutationBritish Journal of Cancer
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Analysis of Germline Gene Copy Number Variants of Patients with Sporadic Pancreatic Adenocarcinoma Reveals Specific Variations

2013

<b><i>Objectives:</i></b> The rapid fatality of pancreatic cancer is, in large part, the result of diagnosis at an advanced stage in the majority of patients. Identification of individuals at risk of developing pancreatic adenocarcinoma would be useful to improve the prognosis of this disease. There is presently no biological or genetic indicator allowing the detection of patients at risk. Our main goal was to identify copy number variants (CNVs) common to all patients with sporadic pancreatic cancer. <b><i>Methods:</i></b> We analyzed gene CNVs in leukocyte DNA from 31 patients with sporadic pancreatic adenocarcinoma and from 93 matched contr…

OncologyMaleCancer Researchmedicine.medical_specialtySettore MED/06 - Oncologia MedicaGene DosageCancer-associated genesBiologyAdenocarcinomaGene dosagePolymorphism Single NucleotideSensitivity and SpecificityGermlineGermline mutationGermline alterationsPolymorphism (computer science)Internal medicinePancreatic cancermedicinepancreatic adenocarcinomaHumansGenetic Predisposition to DiseaseCopy number variationsCopy-number variationGerm-Line MutationGermline alterationAgedCancer-associated geneCopy number variations; Cancer-associated genes; Germline alterations; Sporadic pancreatic cancerCopy number variationCase-control studyGeneral MedicineDNA NeoplasmMiddle Agedmedicine.diseasePancreatic NeoplasmsSporadic pancreatic cancerOncologyTissue Array AnalysisCase-Control StudiesAdenocarcinomaFemale
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Human papillomavirus infections and upper aero-digestive tract cancers: the ARCAGE study.

2013

Human papillomavirus (HPV) is causally implicated in a subset of cancers of the upper aero-digestive tract (UADT). Methods Associations between type-specific HPV antibodies were examined among 1496 UADT cancer case subjects and 1425 control subjects by estimating odds ratios (ORs) in logistic regression analyses adjusted for potential confounders. The agreement between serology and tumor markers of HPV infection, including presence of HPV DNA and p16 expression, were examined in a subset of tumors. Results HPV16 L1 seropositivity was associated with increased risk of oral cavity and oropharyngeal cancer (OR = 1.94, 95% confidence interval [CI] = 1.03 to 3.65; OR = 8.60, 95% CI = 5.21 to 14.…

OncologyMaleCancer Researchmedicine.medical_specialtyvirusesPapillomavirus E7 ProteinsFluorescent Antibody TechniqueBiologyAntibodies ViralPolymerase Chain ReactionSerologyInternal medicinemedicineBiomarkers TumorOdds RatioHumansLaryngeal NeoplasmsAgedNeoplasm StagingHuman papillomavirus 16ConfoundingPapillomavirus InfectionsCase-control studyHPV infectionCancervirus diseasesOdds ratioOncogene Proteins ViralMiddle Agedmedicine.diseasefemale genital diseases and pregnancy complicationsUp-RegulationRepressor ProteinsOropharyngeal NeoplasmsOropharyngeal NeoplasmLogistic ModelsOncologyCase-Control StudiesImmunologyHuman papillomavirus 16; Oropharyngeal Neoplasms; Papillomavirus InfectionsDNA ViralEtiologyFemaleJournal of the National Cancer Institute
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Exposure to UV radiation and risk of Hodgkin lymphoma: A pooled analysis

2013

Ultraviolet radiation (UVR) exposure has been inversely associated with Hodgkin lymphoma (HL) risk, but only inconsistently, only in a few studies, and without attention to HL heterogeneity. We conducted a pooled analysis of HL risk focusing on type and timing of UVR exposure and on disease subtypes by age, histology, and tumor-cell Epstein-Barr virus (EBV) status. Four case-control studies contributed 1320HLcasesand 6381 controls. We estimated lifetime, adulthood, and childhood UVR exposure and history of sunburn and sunlamp use. We used 2-stage estimation with mixed-effects models and weighted pooled effect estimates by inverse marginal variances. We observed statistically significant inv…

OncologyMaleEpstein-Barr Virus InfectionsDNA RepairSunburnSkin PigmentationComorbidityBiochemistryT-Lymphocytes Regulatoryvisual_art.visual_artistimmune system diseaseshemic and lymphatic diseasesYoung adultSunburnAged 80 and overLymphoid NeoplasiaSunbathingintegumentary systemExposure CategoryInside BLOODConfoundingAge FactorsConfounding Factors EpidemiologicHematologyMiddle AgedHodgkin DiseaseEuropeHodgkin lymphoma (HL)visual_artSunlightFemaleAdultmedicine.medical_specialtyAdolescentUltraviolet RaysImmunologyBiologyWhite PeopleYoung AdultSunbathingInternal medicinemedicineHumansUltraviolet radiation (UVR)tumor-cell Epstein-Barr virus (EBV)AgedfungiCase-control studyModels ImmunologicalDose-Response Relationship RadiationCell BiologyOdds ratiomedicine.diseaseRelative riskCase-Control StudiesImmunologyDNA Damage
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A Tetra-Panel of Serum Circulating miRNAs for the Diagnosis of the Four Most Prevalent Tumor Types

2020

The purpose of this study is to clinically validate a series of circulating miRNAs that distinguish between the 4 most prevalent tumor types (lung cancer (LC)

OncologyMaleLung NeoplasmsColorectal cancerlcsh:ChemistryProstate cancer0302 clinical medicinelcsh:QH301-705.5SpectroscopyEarly Detection of CancerAged 80 and over0303 health sciencesArea under the curveGeneral MedicineMiddle AgedEarly diagnosisprostate cancer3. Good healthComputer Science Applications030220 oncology & carcinogenesisArea Under CurveFemaleColorectal NeoplasmsAdultmedicine.medical_specialtyBreast Neoplasmscolorectal cancerCatalysisArticleInorganic Chemistry03 medical and health sciencesBreast cancerbreast cancerInternal medicinemedicineHumansCirculating MicroRNAPhysical and Theoretical ChemistryLung cancerMolecular Biology030304 developmental biologyAgedNeoplasm StagingReceiver operating characteristicbusiness.industryOrganic ChemistryCancerProstatic Neoplasmsmedicine.diseaseCirculating MicroRNAlung cancerlcsh:Biology (General)lcsh:QD1-999ROC CurveCase-Control StudiesMultivariate Analysiscirculating microRNAsbusinessInternational Journal of Molecular Sciences
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Occupational exposure to endocrine disruptors and lymphoma risk in a multi-centric European study

2015

Background: Incidence rates of lymphoma are usually higher in men than in women, and oestrogens may protect against lymphoma. Methods: We evaluated occupational exposure to endocrine disrupting chemicals (EDCs) among 2457 controls and 2178 incident lymphoma cases and subtypes from the European Epilymph study. Results: Over 30 years of exposure to EDCs compared to no exposure was associated with a 24% increased risk of mature B-cell neoplasms (P-trend=0.02). Associations were observed among men, but not women. Conclusions: Prolonged occupational exposure to endocrine disruptors seems to be moderately associated with some lymphoma subtypes. © 2015 Cancer Research UK. All rights reserved.

OncologyMaleendocrine systemCancer Researchmedicine.medical_specialtyanimal structuresLymphomaEpidemiologyJob-exposure matrixchemicalsEndocrine Disruptors03 medical and health sciences0302 clinical medicineSex FactorsSex factorsRisk FactorsInternal medicinehemic and lymphatic diseasesOccupational ExposuremedicineOccupational exposure - endocrine disrupting chemicals (EDCs) - lymphoma riskEndocrine systemHumansurogenital systembusiness.industryIncidence (epidemiology)IncidenceCase-control studynutritional and metabolic diseasespesticidesmedicine.disease030210 environmental & occupational health3. Good healthLymphomaEuropeOccupational DiseasessolventsOncologyMulticenter study030220 oncology & carcinogenesisCase-Control StudiesImmunologyleukaemiaalkylphenolsFemaleOccupational exposurebusinesshormones hormone substitutes and hormone antagonists
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Plasma lipidome patterns associated with cardiovascular risk in the PREDIMED trial: A case-cohort study.

2017

Abstract Background The study of the plasma lipidome may help to better characterize molecular mechanisms underlying cardiovascular disease. The identification of new lipid biomarkers could provide future targets for prevention and innovative therapeutic approaches. In the frame of the PREDIMED trial, our aim was to examine the associations of baseline lipidome patterns or their changes with the risk of clinical CVD events. Methods We included 983 participants in our case-cohort study. The end-point was the incidence of major CVD during 4.8years of median follow-up. We repeatedly measured 202 plasma known lipid metabolites at baseline and after 1-year of intervention. Principal component an…

OncologyMalemedicine.medical_specialtyMediterranean dietCase-cohort030209 endocrinology & metabolismDisease030204 cardiovascular system & hematologyDiet MediterraneanArticleCohort Studies03 medical and health scienceschemistry.chemical_compound0302 clinical medicineRisk FactorsInternal medicineMediterranean dietLipidomicsMedicineHumansNutsOlive OilAgedPrimary preventionbusiness.industryCholesterolIncidence (epidemiology)Case-control studyLipidomeMiddle AgedCardiovascular diseaseLipidschemistryCardiovascular DiseasesCase-Control StudiesLipidomicsPhosphatidylcholinesFemaleCholesterol EstersCardiology and Cardiovascular MedicinebusinessBiomarkersCohort studyFollow-Up Studies
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A genomewide association study of smoking relapse in four European population-based samples.

2013

OBJECTIVES: Genomewide association studies (GWAS) have identified clear evidence of genetic markers for nicotine dependence. Other smoking phenotypes have been tested, but the results are less consistent. The tendency to relapse versus the ability to maintain long-term abstinence has received little attention in genetic studies; thus, our aim was to provide a better biological understanding of this phenotype through the identification of genetic loci associated with smoking relapse. METHODS: We carried out a GWAS on data from two European population-based collections, including a total of 835 cases (relapsers) and 990 controls (abstainers). Top-ranked findings from the discovery phase were …

OncologyMalemedicine.medical_specialtymedia_common.quotation_subjectGenome-wide association studyDiseasePolymorphism Single NucleotideWhite PeopleMeta-Analysis as TopicPolymorphism (computer science)RecurrenceInternal medicineGeneticsmedicineHumansALSPAC; genetics; KORA; PsyCoLaus; SHIP; smokingGeneBiological PsychiatryGenetics (clinical)media_commonDemographyGeneticsbusiness.industrySmokingAbstinenceMiddle AgedPhenotypePsychiatry and Mental healthGenetic markerCase-Control StudiesGenomewide associationFemalebusinessGenome-Wide Association StudyPsychiatric genetics
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New methods to improve the safety assessment of cryopreserved ovarian tissue for fertility preservation in breast cancer patients

2015

Objective To develop a novel molecular panel of markers to detect breast cancer (BC) disseminated malignant cells in ovarian tissue, and to improve the safety of ovarian tissue transplantation. Design Experimental study. Setting University hospital. Patient(s) Ten ovarian biopsies from healthy patients, 13 biopsies with diagnosed BC metastasis, and 4 biopsies from primary BC tumor for designing a diagnostic panel of BC cell contamination; 60 ovarian biopsies from BC patients undergoing fertility preservation for validating the panel. Animal(s) Female nude mice. Intervention(s) A novel panel for BC malignant cell detection by reverse-transcription polymerase chain reaction (RT-PCR), inmmunoh…

OncologyPathologyBiopsyMetastasisPregnancyRisk FactorsMedicineOvarian tissue cryopreservationFertility preservationMUC1Ovarian Neoplasmsmedicine.diagnostic_testReverse Transcriptase Polymerase Chain ReactionMammaglobin AFertility PreservationObstetrics and GynecologyMiddle AgedImmunohistochemistrymedicine.anatomical_structureNeoplasm MicrometastasisHeterograftsFemaleInfertility FemaleAdultmedicine.medical_specialtyReproductive Techniques AssistedMice NudeBreast NeoplasmsOvaryBreast cancerPredictive Value of TestsInternal medicineBiopsyBiomarkers TumorAnimalsHumansNeoplasm InvasivenessAgedGlycoproteinsCryopreservationbusiness.industryOvaryMucinsMembrane Transport ProteinsReproducibility of Resultsmedicine.diseaseTransplantationFertilityReproductive MedicineCase-Control StudiesCarrier ProteinsbusinessFertility and Sterility
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