Search results for "cd105"
showing 5 items of 5 documents
A Library-Based Screening Strategy for the Identification of DARPins as Ligands for Receptor-Targeted AAV and Lentiviral Vectors
2021
Delivering genes selectively to the therapeutically relevant cell type is among the prime goals of vector development. Here, we present a high-throughput selection and screening process that identifies designed ankyrin repeat proteins (DARPins) optimally suited for receptor-targeted gene delivery using adeno-associated viral (AAV) and lentiviral (LV) vectors. In particular, the process includes expression, purification, and in situ biotinylation of the extracellular domains of target receptors as Fc fusion proteins in mammalian cells and the selection of high-affinity binders by ribosome display from DARPin libraries each covering more than 1012 variants. This way, DARPins specific for the …
Airway epithelial dysfunction and mesenchymal transition in chronic obstructive pulmonary disease: Role of Oct-4
2021
Abstract The airway epithelium is a dynamic tissue that undergoes slow but constant renewal. Dysregulation of airway epithelial function related to cigarette smoke exposure plays an important role in the pathophysiology of COPD. Oct4 is a transcription factor responsible for maintaining cellular self-renewal and regeneration, and CD146 and CD105/Endoglin are adhesion molecules involved in cell proliferation, differentiation, epithelial-mesenchymal-transition and tissue remodeling. Bronchial biopsy specimens (BBs) were obtained from 7 healthy controls (HC) and 10 COPD and subjected to paraffin embedding; BBs from HC were also used for epithelial cell expansion and pHBEC/ALI (air-liquid inter…
Role of chronic exsposure to cigarette smoke on endoglin/CD105 expression in airway epithelium.
2015
Dysregulation of airway epithelial cell function related to cigarette smoke exposure plays an important role in the pathophysiology of COPD and is associated to tissue damage and disease severity. CD105 is a component of the receptor complex of TGF-β, a pleiotropic cytokine involved in cellular proliferation, differentiation and migration. CD105 regulates the expression of different components of the extracellular matrix suggesting a role of CD105 in cellular transmigration and remodeling processes. The aim of the present study was to investigate the expression of endoglin/CD105 in airway epithelium of COPD patients and its involvement in tissue remodeling and progression of COPD. We evalua…
Evaluación de marcadores inmunohistoquímicos de angiogénesis tumoral como factor pronóstico en el adenocarcinoma pulmonar en estadio IA
2019
El cáncer de pulmón (CP) es la neoplasia más frecuentemente diagnosticada y la que más muertes causa en el mundo. A pesar de los avances disponibles en el diagnóstico y el tratamiento aun presenta una tasa de supervivencia entorno al 15%. El objetivo de este trabajo fue investigar y correlacionar factores morfológicos del ADC de pulmón con la expresión de marcadores de angiogénesis tumoral como factor pronóstico, en pacientes resecados y clasificados como T1a-b-cN0M0 patológico, de la 8ª clasificación TNM. Por tanto, se realizó un proyecto de investigación clínica, de naturaleza observacional y analítico, con recogida de datos retrospectivos de los casos a partir de los archivos de historia…
CD34 and CD105 Microvessels in Resected Bone Specimen May Implicate Wound Healing in MRONJ
2021
Clinical treatment outcome of MRONJ (medication-related osteonecrosis of the jaw) surgery despite radical osseous removal and primary closure healing still shows differences in terms of outcome and disease recurrence. The study aims to assess the rate of angiogenesis of MRONJ lesions in order to understand the impact of angiogenesis and neoangiogenesis status on MRONJ surgical treatment outcome. This is the first study correlating microvessel density with prognosis in MRONJ surgically-treated patients. The immunohistochemical expression of CD34 and CD105 in MRONJ specimens obtained from surgically-treated patients was evaluated. The most vascularized areas detected by CD34 and CD105 were se…