Search results for "centri"
showing 10 items of 869 documents
Differential expression and interaction with the visual G-protein transducin of centrin isoforms in mammalian photoreceptor cells.
2004
Photoisomerization of rhodopsin activates a heterotrimeric G-protein cascade leading to closure of cGMP-gated channels and hyperpolarization of photoreceptor cells. Massive translocation of the visual G-protein transducin, Gt, between subcellular compartments contributes to long term adaptation of photoreceptor cells. Ca(2+)-triggered assembly of a centrin-transducin complex in the connecting cilium of photoreceptor cells may regulate these transducin translocations. Here we demonstrate expression of all four known, closely related centrin isoforms in the mammalian retina. Interaction assays revealed binding potential of the four centrin isoforms to Gtbetagamma heterodimers. High affinity b…
Ekskurs XI. Sprofanowana świątynia (Ez 8,1-18) i dolina suchych kości (Ez 37,1-14) w świetle retoryki hebrajskiej
2021
Kontekstem badań było to, że komentatorzy Księgi Ezechiela nie są zgodni w sprawie struktury badanych tekstów i proponują odmienne schematy. Celem badań stało się odkrycie struktury, którą starożytny autor natchniony zawarł w tekście. By osiągnąć założony cel, zastosowano metodę retoryki hebrajskiej, którą opracował Roland Meynet. W wyniku przeprowadzonych badań udało się odkryć, że sprofanowana świątynia ma strukturę paralelno-koncentryczną, składającą się z 9 elementów (A, B, C, D, E, D’, C’, B’, A’), natomiast dolina suchych kości też ma strukturę paralelno-koncentryczną, na którą składa się 5 elementów (A, B, C, B’, A’). Osiągnięte wyniki pozwoliły wyciągnąć wspólny wniosek dla dwóch ba…
The yeast histone acetyltransferase A2 complex, but not free Gcn5p, binds stably to nucleosomal arrays.
2000
We have investigated the structural basis for the differential catalytic function of the yeast Gcn5p-containing histone acetyltransferase (HAT) A2 complex and free recombinant yeast Gcn5p (rGcn5p). HAT A2 is shown to be a unique complex that contains Gcn5p, Ada2p, and Ada3p, but not proteins specific to other related HAT A complexes, e.g. ADA, SAGA. Nevertheless, HAT A2 produces the same unique polyacetylation pattern of nucleosomal substrates reported previously for ADA and SAGA, demonstrating that proteins specific to the ADA and SAGA complexes do not influence the enzymatic activity of Gcn5p within the HAT A2 complex. To investigate the role of substrate interactions in the differential …
Understanding the release and metabolism of aroma compounds using micro-volume saliva samples by ex vivo approaches
2018
International audience; This study investigated the behaviour of key aroma compounds in the presence of human saliva (200 mu L) from different individuals (n = 3) submitted or not to centrifugation (whole vs clarified saliva). HS-GC results showed that human saliva strongly decreased the release of carbonyl compounds (aldehydes and ketones). This effect was dependent on i) the structure of the aroma compounds and ii) the saliva composition. Whole saliva exerted a higher effect than clarified saliva on aroma compounds. Moreover, this effect was individual-dependent and related to the total protein content and the total antioxidant capacity of saliva. HS-SPME and LLE-GC/MS analyses revealed t…
6 Self-organising Maps To Analyse Effects Of Low Intensity Concentric Combined With Blood Flow Restriction
2014
Introduction Self-Organising Maps (SOM) are a type of Artificial Neural Network (ANN) model used to visualise multidimensional data [Kohonen-00]. SOM provides a correspondence between the original N-dimensional space and a twodimensional space [Haykin-08]. In the case presented in this work, this means that two subjects with similar values of the different variables should appear near in the two-dimensional space [Kohonen-00], [Haykin-08]. Method In order to test this method, 15 athletes were selected. Subjects’ measurements were performed in three different periods: before the exercise test, immediately after performing the exercise and twenty-four hours later. The control measures were th…
A serum fucolectin isolated and characterized from sea bass Dicentrarchus labrax
2001
A lectin specific for fucose and galactose was isolated by affinity chromatography on Sepharose CL-6B from the serum of Dicentrarchus labrax. The hemagglutinating activity against rabbit erythrocytes was calcium-independent, and reached its maximum at 37 degrees C. Two protein components were found in the hemagglutinating fractions eluted from the Sepharose column. Only the 34 kDa component (DLL2) eluted from the polyacrylamide gels (SDS-PAGE) showed agglutinating activity against rabbit erythrocytes. SDS-PAGE, in non-reducing conditions, revealed a single 66 kDa protein that reacted with antibodies to the 34 kDa component. Therefore, a dimeric structure stabilized by disulfide bonds can be…
Improving Usability of Location-Based Services with User-Centric Data Querying
2004
In this paper, we describe a computational model that produces a dataset which we consider to be an appropriate response for “give me a description of my neighborhood” type of queries in location-based services Our attempt is to reflect the abstraction ability of a specific user, and in this way to maximize the usability of the service under restrictions on data volume posed by technical, economical and cognitive factors of mobile environments We also present the results of instantiating the model for a simple LBS.
Analisi teorico-sperimentale del comportamento flessionale di colonne in calcestruzzo fibrorinforzato
2009
CRITERI E TECNICHE DI VALUTAZIONE E RIDUZIONE DELLA VULNERABILITÀ SISMICA DEL CENTRO STORICO
2016
Una valutazione della vulnerabilità sismica, sia attraverso approcci fenomenologici che attraverso più accurati modelli meccanici non può prescindere dalla conoscenza (il più possibile accurata) di tali caratteristiche del costruito, che può essere acquisita spesso soltanto attraverso campagne di rilievo e di indagini sul costruito. In questo contesto, vengono inizialmente descritte le caratteristiche salienti del centro storico di Modica, per poi passare in rassegna i metodi di valutazione della vulnerabilità sismica a scala territoriale; infine verranno presentate alcune tecniche di mitigazione della vulnerabilità sismica, e presidi antisismici da mettere in atto per evitare che trasforma…