Search results for "chain"

showing 10 items of 4255 documents

Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture.

2017

Thioredoxins (Trxs), key components of cellular redox regulation, act by controlling the redox status of many target proteins, and have been shown to play an essential role in cell survival and growth. The presence of a Trx system in the nucleus has received little attention in plants, and the nuclear targets of plant Trxs have not been conclusively identified. Thus, very little is known about the function of Trxs in this cellular compartment. Previously, we studied the intracellular localization of PsTrxo1 and confirmed its presence in mitochondria and, interestingly, in the nucleus under standard growth conditions. In investigating the nuclear function of PsTrxo1 we identified proliferati…

0106 biological sciences0301 basic medicineTFs transcription factorsOverexpressionBiologíaBiFC bimolecular fluorescence complementationClinical BiochemistryCell Culture TechniquesTobacco BY-2 cells01 natural sciencesBiochemistryTBY-2 tobacco bright yellow-2DTT 14-dithiothreitolBimolecular fluorescence complementationThioredoxinsGene Expression Regulation PlantTrx thioredoxinlcsh:QH301-705.5GFP green fluorescent proteinlcsh:R5-920biologyProliferating cell nuclear antigen (PCNA)Cell cycleGlutathione3. Good healthCell biologyMitochondriaNTR NADPH thioredoxin reductaseProtein TransportDEM diethyl maleateRT-qPCR Reverse transcription quantitative polymerase chain reactionThioredoxinlcsh:Medicine (General)Oxidation-ReductionAMS 4-acetamido-4-maleimidylstilbene-22-disulfonic acidResearch PaperPCNA proliferating cell nuclear antigenOex overexpressingCell cycleNucleusThioredoxin o103 medical and health sciencesROS reactive oxygen speciesDownregulation and upregulationProliferating Cell Nuclear AntigenTobaccoDAPI 46-diamidine-2-phenylindolmCBM monochlorobimaneCellular compartmentCell NucleusCell growthOrganic ChemistryBotánicaPeasMolecular biologyYFP yellow fluorescent proteinProliferating cell nuclear antigenTBS Tris-buffered salineOD optical density030104 developmental biologylcsh:Biology (General)Cell cultureRNA reactive nitrogen speciesbiology.proteinPrx peroxiredoxinBSA bovine serum albumin010606 plant biology & botanyRedox biology
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Survey of five major grapevine viruses infecting Blatina and Žilavka cultivars in Bosnia and Herzegovina

2021

The sanitary status of grapevines has not yet been considered sufficiently in vineyards throughout Bosnia and Herzegovina (BiH). An extensive survey of five major grapevine viruses in the country was carried out in 2019. A total of 630 samples from the two dominant autochthonous cultivars, named Žilavka and Blatina, were tested by DAS-ELISA for the presence of grapevine leafroll-associated viruses (GLRaV-1 and 3), grapevine fleck virus (GFkV), grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV). Eighty-eight % of the samples were positive for at least one virus, and all five viruses were detected, thought with different incidence, i.e. GLRaV-3 (84%), GFLV (43%), GLRaV-1 (14%), GFk…

0106 biological sciences0301 basic medicineVeterinary medicineArtificial Gene Amplification and ExtensionDisease VectorsPathology and Laboratory MedicinePolymerase Chain Reaction01 natural sciencesPlant VirusesMedical ConditionsXiphinemaMedicine and Health SciencesVitisCultivarNematode InfectionsFlowering PlantsPhylogenyData Managementeducation.field_of_studyMultidisciplinarybiologyPhylogenetic treeQREukaryotaPhylogenetic AnalysisGrapevine fanleaf virusPlantsPhylogeneticsInfectious DiseasesMedical MicrobiologyViral PathogensVirusesMedicineRNA ViralGrapevineViral VectorsPathogensPlant ViruseResearch ArticleComputer and Information SciencesSciencePopulationPlant DiseaseResearch and Analysis Methodsno key wordsMicrobiologyVirusArabis mosaic virus03 medical and health sciencesVirologyParasitic DiseasesEvolutionary SystematicsMolecular Biology TechniqueseducationMolecular BiologyMicrobial PathogensTaxonomyPlant DiseasesBosnia and HerzegovinaEvolutionary BiologyGenetic diversityOrganismsBiology and Life SciencesSettore AGR/12 - Patologia VegetaleReverse Transcriptase-Polymerase Chain Reactionbiology.organism_classificationVector-Borne DiseasesSpecies Interactions030104 developmental biologyViral Transmission and Infection010606 plant biology & botany
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Begomoviruses Infecting Tomato Crops in Panama

2015

The key regions in Panama involved in open field- and greenhouse-grown commercial tomato production, including the Chiriquí, Veraguas, Herrera, Los Santos, Coclé and Panama Oeste provinces, were surveyed for the incidence and distribution of begomoviruses in the growing seasons of 2011 and 2012. The surveys took place in 14 of the 51 districts of the above-mentioned provinces and comprised all relevant tomato production areas of the provinces. A total of 28 tomato plots were surveyed. The exact location of each plot was geo-referenced using a hand-held Global Positioning System unit. In total, 319 individual tomato plants (181 in 2011 and 138 in 2012) were sampled. Plants displayed diverse …

0106 biological sciences0301 basic medicineVeterinary medicinePhysiologyGrowing seasonTomato leaf curl Sinaloa viruPlant ScienceBiology01 natural scienceslaw.invention03 medical and health sciencesSolanum lycopersicumlawGenusBotanyTomato yellow mottle viruGeneticsLeaf sizePolymerase chain reactionPanamafungiBegomovirusSettore AGR/12 - Patologia Vegetalefood and beveragesbiology.organism_classificationPolymerase chain reactionTomato yellow mottle virus030104 developmental biologyKey (lock)Potato yellow mosaic Panama viruAgronomy and Crop Science010606 plant biology & botanyJournal of Phytopathology
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Insect Vectors (Hemiptera: Cixiidae) and Pathogens Associated with the Disease Syndrome “Basses Richesses” of Sugar Beet in France

2019

International audience; The syndrome “basses richesses” (SBR) is a disease of sugar beet in eastern France associated with two phloem-restricted, nonculturable plant pathogens: a stolbur phytoplasma and a γ-3 proteobacterium, here called SBR bacterium. Three planthopper (Hemiptera: Cixiidae) species were found to live near and within sugar beet fields in eastern France: Cixius wagneri, Hyalesthes obsoletus, and Pentastiridius leporinus. The role of these planthoppers in spreading the two pathogens to sugar beet was studied. Based on its abundance and high frequency of infection with the SBR bacterium, P. leporinus was considered to be the economic vector of SBR disease. C. wagneri, the prim…

0106 biological sciencesBASSES RICHESSES SYNDROME OF SUGAR BEETHomopteraEXPERIMENTAL TRANSMISSIONCIXIIDAEPlant Science01 natural sciencesHEMIPTERADETECTION03 medical and health sciencesPlanthopperBotanySugarPOLYMERASE CHAIN REACTION RESTRICTED FRAGMENT LENGH POLYMORPHISM030304 developmental biology2. Zero hunger0303 health sciencesbiologyPHLOEM LIMITED BACTERIAfungifood and beveragesLeporinusbiology.organism_classificationCixiidae[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacyHYALESTHES OBSOLETUSINSECTEGAMMA-3-PROTEOBACTERIAPhytoplasmaSTOLBUR PHYTOPLASMAVECTORSSugar beetCIXIUS WAGNERICHARACTERIZATIONAgronomy and Crop ScienceConvolvulusPENTASTIRIDIUS LEPORINUS010606 plant biology & botany
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The Bias of combining variables on fish's aggressive behavior studies.

2019

Made available in DSpace on 2019-10-06T16:27:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-07-01 Quantifying animal aggressive behavior by behavioral units, either displays or attacks, is a common practice in animal behavior studies. However, this practice can generate a bias in data analysis, especially when the variables have different temporal patterns. This study aims to use Bayesian Hierarchical Linear Models (B-HLMs) to analyze the feasibility of pooling the aggressive behavior variables of four cichlids species. Additionally, this paper discusses the feasibility of combining variables by examining the usage of different sample sizes and family distributions to aggressive …

0106 biological sciencesBayesian probabilityPosterior probabilityBayesian analysisPoisson distribution010603 evolutionary biology01 natural sciencesBehavioral Neurosciencesymbols.namesakeBiasPrior probabilityStatisticsAnimals0501 psychology and cognitive sciences050102 behavioral science & comparative psychologyPterophyllum scalareMathematicsProbabilitybiologyBehavior Animal05 social sciencesMultilevel modelBayes TheoremGeneral MedicineCichlidsbiology.organism_classificationAggressive behaviourMarkov ChainsAggressionVariable (computer science)Sample size determinationData Interpretation StatisticalsymbolsAnimal Science and ZoologyPooled dataMonte Carlo MethodBehavioural processes
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Zooplankton abundance: A neglected key element in the evaluation of reservoir water quality

2018

Abstract Based on our results, we propose the use of zooplankton abundance (density or biomass) as an indicator to complement the information currently being used concerning the quality of water in reservoirs. Until now, the Water Framework Directive (EU) for lakes and reservoirs has not included zooplankton because the classification of the water trophic state is based on a bottom-up model: an increase in nutrients implies an increase in primary producers and, therefore, poorer water quality. The use of zooplankton has recently been claimed due to their sensitivity to environmental changes and their control over primary producers. From our work, carried out from 2006 to 2009 (summer and wi…

0106 biological sciencesBiomass (ecology)Primary producersEcology010604 marine biology & hydrobiologyfungiAquatic Science010603 evolutionary biology01 natural sciencesZooplanktonhumanitiesFood chainWater Framework DirectivePhytoplanktonEnvironmental scienceWater qualityTrophic levelLimnologica
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Fish introductions and light modulate food web fluxes in tropical streams: a whole-ecosystem experimental approach.

2016

Decades of ecological study have demonstrated the importance of top-down and bottom-up controls on food webs, yet few studies within this context have quantified the magnitude of energy and material fluxes at the whole-ecosystem scale. We examined top-down and bottom-up effects on food web fluxes using a field experiment that manipulated the presence of a consumer, the Trinidadian guppy Poecilia reticulata, and the production of basal resources by thinning the riparian forest canopy to increase incident light. To gauge the effects of these reach-scale manipulations on food web fluxes, we used a nitrogen (15 N) stable isotope tracer to compare basal resource treatments (thinned canopy vs. co…

0106 biological sciencesCanopyNeotropicsFood ChainLightPopulation DynamicsContext (language use)010603 evolutionary biology01 natural sciencesRiverstrophic linkagesAnimalsEcosystemTrinidad guppyBiomassEcology Evolution Behavior and Systematicstop-down and bottom-up effectsTrophic levelTropical ClimateDetritusbiologyEcology010604 marine biology & hydrobiologynitrogen fluxFishesWaterbiology.organism_classificationFood webGuppyreach-scale experimentstable isotope tracersTrinidad and TobagoBenthic zoneta1181stream food webbenthic macroinvertebratesprimary productionEcology
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The Impact of Variations in the Climate on Seasonal Dynamics of Phytoplankton

2009

Phytoplankton, an assemblage of suspended, primarily autotrophic single cells and colonies, forms part of the base of the pelagic food chain in lakes. The responses of phytoplankton to anthropogenic pressures frequently provide the most visible indication of a long-term change in water quality. Several attributes related to the growth and composition of phytoplankton, such as their community structure, abundance as well as the frequency and the intensity of blooms, are included as indicators of water quality in the Water Framework Directive. The growth and seasonal succession of phytoplankton is regulated by a variety of external as well as internal factors (Reynolds et al., 1993; Reynolds,…

0106 biological sciencesCatchment ModellingClimate Change010604 marine biology & hydrobiologyCommunity structureClimate changePelagic zoneLake Modelling15. Life on landSpring bloom010603 evolutionary biology01 natural sciences6. Clean waterFood chainOceanographyWater column13. Climate actionddc:570Water QualityClimatologyPhytoplanktonEnvironmental science14. Life underwaterWater quality
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Constitutive expression of clathrin hub hinders elicitor-induced clathrin-mediated endocytosis and defense gene expression in plant cells.

2012

International audience; Endocytosis has been recently implicated in the signaling network associated with the recognition of microbes by plants. In a previous study, we showed that the elicitor cryptogein was able to induce clathrin-mediated endocytosis (CME) in tobacco suspension cells. Herein, we investigate further the induced CME by means of a GFP-tagged clathrin light chain and a CME inhibitor, the hub domain of clathrin heavy chain. Hub constitutive expression does affect neither cell growth nor constitutive endocytosis but abolishes cryptogein-induced CME. Such an inhibition has no impact on early events in the cryptogein signaling pathway but reduces the expression of defense-associ…

0106 biological sciencesCell signaling[SDV]Life Sciences [q-bio]Recombinant Fusion ProteinseducationBiophysicsGene Expressionbright yellow-2BiologyEndocytosisGenes Plant01 natural sciencesBiochemistryClathrincryptogeinCell LineFungal Proteins03 medical and health sciencesMicroscopy Electron TransmissionStructural BiologyGene expressionTobaccoGeneticscell signalingRNA MessengerMolecular Biology030304 developmental biologyPlant Proteins0303 health sciencesCell growthCell MembraneCell BiologyReceptor-mediated endocytosisPlants Genetically ModifiedClathrinEndocytosisElicitorCell biologyRNA PlantClathrin Heavy Chains[SDE]Environmental Sciencesbiology.proteinClathrin Light ChainsSignal transduction010606 plant biology & botanySignal TransductionFEBS letters
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Synchronous attack is advantageous: mixed genotype infections lead to higher infection success in trematode parasites

2011

Co-infecting parasite genotypes typically compete for host resources limiting their fitness. The intensity of such competition depends on whether parasites are reproducing in a host, or using it primarily as a transmission vehicle while not multiplying in host tissues (referred to as ‘competition hypothesis’). Alternatively, simultaneous attack and co-infection by several parasite genotypes might facilitate parasite infection because such a diverse attack could present an additional challenge to host immune defence (referred to as ‘facilitation hypothesis’). We tested the competition hypothesis by comparing the production of transmission stages (cercariae) from snails infected with one or …

0106 biological sciencesCompetitive BehaviorGenotypemedia_common.quotation_subjectZoologyTrematode InfectionsBiology010603 evolutionary biology01 natural sciencesPolymerase Chain ReactionGeneral Biochemistry Genetics and Molecular BiologyCompetition (biology)Host-Parasite Interactions03 medical and health sciencesGenotypeParasite hostingAnimalsCercariaResearch ArticlesFinland030304 developmental biologyGeneral Environmental Sciencemedia_commonLymnaea0303 health sciencesAnalysis of VarianceGeneral Immunology and MicrobiologyHost (biology)Transmission (medicine)ReproductionGenetic VariationGeneral Medicinebiology.organism_classificationObligate parasiteOncorhynchus mykissImmunologyFacilitationTrematodaTrematodaGeneral Agricultural and Biological SciencesProceedings of the Royal Society B-Biological Sciences
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