Search results for "cid"
showing 10 items of 19479 documents
Tecnica dell'acidificazione dei vini
2008
Building Resilience due to violence: The struggle of Mexican Communities
2015
In Psychology the term resilience refers to man’s ability to respond to stressful events in a positive manner. Cases of feminicide in Mexican society provide significant information regarding the relationship between violence and the development of a capacity for resistance in order to preserve life in the long term. Given that the promotion of empowered actions is closely related with issues of community, power and precariousness, this article will discuss cultural and socio-economic factors in institutional violence against Mexican women and will provide a comprehensive look at a theoretical framework for understanding resilience within this context, starting from the relationship between…
Protección integral de la mujer con diversidad funcional en supuestos de violencia de género
2016
"Protección integral de la mujer con diversidad funcional en supuestos de violencia de género" es la culminación de cuatro intensos años de apasionante investigación jurídica, dirigidos por la Doctora Elena Martínez García, la cual partió de una temática especialmente novedosa en el ámbito del derecho, pero que nos inducía a pensar que detrás de ella existía una realidad muchas veces silenciada. A partir de cuestiones tan concretas como igualdad, género, discapacidad o violencia machista, hemos visto como en la última fase de nuestro trabajo, el mismo ha terminado por coincidir con un legislador y unas políticas sociales mucho más abiertas e inclusivas, permitiéndonos recorrer en paralelo y…
¿Existe violencia en las parejas del mismo sexo?
2010
El presente artículo intenta dar respuestas a preguntas relacionadas con la existencia o inexistencia de violencia en parejas del mismo sexo. Al igual que intenta explicar cómo es esta violencia, a qué es debida, y si es similar a la violencia que ejercen los hombres contra las mujeres. Concluyendo el mismo, con diferentes propuestas de trabajo en relación a la violencia que se da dentro de las parejas del mismo sexo a nivel individual, familiar, grupal y comunitario. This article sets out to provide replies to questions connected with the existence or nonexistence of violence in same-sex couples. It likewise attempts to explain what this violence is like, what its causes are, and whether t…
Femminicidi a processo. Dati, stereotipi e narrazioni della violenza di genere
2021
Il volume espone i risultati di una ricerca sulle rappresentazioni del femminicidio in ambito giudiziario in Italia, illustrandone il quadro teorico, le scelte metodologiche, l’impianto analitico, gli strumenti. La tematica del potere e della diseguaglianza tra i sessi è analizzata attraverso le definizioni fornitene in ambito giuridico e nei codici penali, esplorandone i significati sociali e questionando i possibili ancoraggi politici ed economici, attraverso il modello del “campo giuridico” di Bourdieu quale luogo di espressione di una forza simbolica che contende il potere ad altri “campi sociali”. L’indagine empirica è condotta attraverso un triplice livello di approfondimento di natur…
Single-chain antibodies against a plant viral RNA-dependent RNA polymerase confer virus resistance.
2004
Crop loss due to viral diseases is still a major problem for agriculture today. We present a strategy to achieve virus resistance based on the expression of single-chain Fv fragments (scFvs) against a conserved domain in a plant viral RNA-dependent RNA polymerase (RdRp), a key enzyme in virus replication. The selected scFvs inhibited complementary RNA synthesis of different plant virus RdRps in vitro and virus replication in planta. Moreover, the scFvs also bound to the RdRp of the distantly related hepatitis C virus. T(1) and T(2) progeny of transgenic lines of Nicotiana benthamiana expressing different scFvs either in the cytosol or in the endoplasmic reticulum showed varying degrees of r…
Modulation of Hepatitis C Virus NS5A Hyperphosphorylation by Nonstructural Proteins NS3, NS4A, and NS4B
1999
NS5A of the hepatitis C virus (HCV) is a highly phosphorylated protein involved in resistance against interferon and required most likely for replication of the viral genome. Phosphorylation of this protein is mediated by a cellular kinase(s) generating multiple proteins with different electrophoretic mobilities. In the case of the genotype 1b isolate HCV-J, in addition to the basal phosphorylated NS5A (designated pp56), a hyperphosphorylated form (pp58) was found on coexpression of NS4A (T. Kaneko, Y. Tanji, S. Satoh, M. Hijikata, S. Asabe, K. Kimura, and K. Shimotohno, Biochem. Biophys. Res. Commun. 205:320‐326, 1994). Using a comparative analysis of two full-length genomes of genotype 1b…
Functional properties of a monoclonal antibody inhibiting the hepatitis C virus RNA-dependent RNA polymerase.
2001
The hepatitis C virus (HCV) RNA-dependent RNA polymerase (RdRp), represented by nonstructural protein 5B (NS5B), has recently emerged as a promising target for antiviral intervention. Here, we describe the isolation, functional characterization, and molecular cloning of a monoclonal antibody (mAb) inhibiting the HCV RdRp. This mAb, designated 5B-12B7, binds with high affinity to a conformational epitope in the palm subdomain of the HCV RdRp and recognizes native NS5B expressed in the context of the entire HCV polyprotein or subgenomic replicons. Complete inhibition of RdRp activity in vitro was observed at equimolar concentrations of NS5B and mAb 5B-12B7, whereas RdRp activities of classica…
Viral and cellular determinants of hepatitis C virus RNA replication in cell culture.
2003
Studies on the replication of hepatitis C virus (HCV) have been facilitated by the development of selectable subgenomic replicons replicating in the human hepatoma cell line Huh-7 at a surprisingly high level. Analysis of the replicon population in selected cells revealed the occurrence of cell culture-adaptive mutations that enhance RNA replication substantially. To gain a better understanding of HCV cell culture adaptation, we characterized conserved mutations identified by sequence analysis of 26 independent replicon cell clones for their effect on RNA replication. Mutations enhancing replication were found in nearly every nonstructural (NS) protein, and they could be subdivided into at …
Biochemical properties of hepatitis C virus NS5B RNA-dependent RNA polymerase and identification of amino acid sequence motifs essential for enzymati…
1997
The NS5B protein of the hepatitis C virus (HCV) is an RNA-dependent RNA polymerase (RdRp) (S.-E. Behrens, L. Tomei, and R. De Francesco, EMBO J. 15:12-22, 1996) that is assumed to be required for replication of the viral genome. To further study the biochemical and structural properties of this enzyme, an NS5B-hexahistidine fusion protein was expressed with recombinant baculoviruses in insect cells and purified to near homogeneity. The enzyme was found to have a primer-dependent RdRp activity that was able to copy a complete in vitro-transcribed HCV genome in the absence of additional viral or cellular factors. Filter binding assays and competition experiments showed that the purified enzym…