Search results for "circular"
showing 10 items of 856 documents
The coat protein of prunus necrotic ringspot virus specifically binds to and regulates the conformation of its genomic RNA
2003
AbstractBinding of coat protein (CP) to the 3′ nontranslated region (3′-NTR) of viral RNAs is a crucial requirement to establish the infection of Alfamo- and Ilarviruses. In vitro binding properties of the Prunus necrotic ringspot ilarvirus (PNRSV) CP to the 3′-NTR of its genomic RNA using purified E. coli- expressed CP and different synthetic peptides corresponding to a 26-residue sequence near the N-terminus were investigated by electrophoretic mobility shift assays. PNRSV CP bound to, at least, three different sites existing on the 3′-NTR. Moreover, the N-terminal region between amino acid residues 25 to 50 of the protein could function as an independent RNA-binding domain. Single exchan…
Bivalve mollusks shells valorisation and recycling: market potentiality and novel building products
2023
Construction is a fundamental driver of the world economy, recognized as a sector that generates a great contribution to the social and economic development. At the same time, it shows an enormous environmental impact through an unstoppable consumption of non-renewable resources, a high energy consumption, and an associated atmospheric emission of pollutant. Consequently, the research and development of novel and green production processes and sustainable building materials is of paramount importance. The sustainability of materials and production processes could be improved by enhancing and reusing wastes from various industrial sectors. In this paper, the fishery sector will be analysed, …
Suffixes, Conjugates and Lyndon Words
2013
In this paper we are interested in the study of the combinatorial aspects connecting three important constructions in the field of string algorithms: the suffix array, the Burrows-Wheeler transform (BWT) and the extended Burrows-Wheeler transform (EBWT). Such constructions involve the notions of suffixes and conjugates of words and are based on two different order relations, denoted by $\plex$ and $\pom$, that, even if strictly connected, are quite different from the computational point of view. In this study an important role is played by Lyndon words. In particular, we improve the upper bound on the number of symbol comparisons needed to establish the $\pom$ order between two primitive wo…
Adaptive independent vector analysis for multi-subject complex-valued fMRI data.
2017
Abstract Background Complex-valued fMRI data can provide additional insights beyond magnitude-only data. However, independent vector analysis (IVA), which has exhibited great potential for group analysis of magnitude-only fMRI data, has rarely been applied to complex-valued fMRI data. The main challenges in this application include the extremely noisy nature and large variability of the source component vector (SCV) distribution. New method To address these challenges, we propose an adaptive fixed-point IVA algorithm for analyzing multiple-subject complex-valued fMRI data. We exploited a multivariate generalized Gaussian distribution (MGGD)- based nonlinear function to match varying SCV dis…
On the way to ‘zero waste’ management: Recovery potential of elements, including rare earth elements, from fine fraction of waste
2018
Abstract Existing schemes of solid waste handling have been improved implementing advanced systems for recovery and reuse of various materials. Nowadays, the ‘zero waste’ concept is becoming more topical through the reduction of disposed waste. Recovery of metals, nutrients and other materials that can be returned to the material cycles still remain as a challenge for future. Landfill mining (LFM) is one of the approaches that can deal with former dumpsites, and derived materials may become important for circular economy within the concept ‘beyond the zero waste’. Perspectives of material recovery can include recycling of critical industrial metals, including rare earth elements (REEs). The…
Small Scale Gasification Application and Perspectives in Circular Economy
2018
Abstract Gasification is the process converting solid fuels as coal and organic plant matter, or biomass into combustible gas, called syngas. Gasification is a thermal conversion process using carbonaceous fuel, and it differs substantially from other thermal processes such as incineration or pyrolysis. The process can be used with virtually any carbonaceous fuel. It is an endothermic thermal conversion process, with partial oxidation being the dominant feature. Gasification converts various feedstock including waste to a syngas. Instead of producing only heat and electricity, synthesis gas produced by gasification may be transformed into commercial products with higher value as transport f…
Mutational analysis of the RNA-binding domain of the Prunus necrotic ringspot virus (PNRSV) movement protein reveals its requirement for cell-to-cell…
2005
AbstractThe movement protein (MP) of Prunus necrotic ringspot virus (PNRSV) is required for cell-to-cell movement. MP subcellular localization studies using a GFP fusion protein revealed highly punctate structures between neighboring cells, believed to represent plasmodesmata. Deletion of the RNA-binding domain (RBD) of PNRSV MP abolishes the cell-to-cell movement. A mutational analysis on this RBD was performed in order to identify in vivo the features that govern viral transport. Loss of positive charges prevented the cell-to-cell movement even though all mutants showed a similar accumulation level in protoplasts to those observed with the wild-type (wt) MP. Synthetic peptides representin…
C-terminal amino acids are essential for human heat shock protein 70 dimerization
2014
The human inducible heat shock protein 70 (hHsp70), which is involved in several major pathologies, including neurodegenerative disorders and cancer, is a key molecular chaperone and contributes to the proper protein folding and maintenance of a large number of protein structures. Despite its role in disease, the current structural knowledge of hHsp70 is almost exclusively based on its Escherichia coli homolog, DnaK, even though these two proteins only share ~50 % amino acid identity. For the first time, we describe a complete heterologous production and purification strategy that allowed us to obtain a large amount of soluble, full-length, and non-tagged hHsp70. The protein displayed both …
A circular economy: an analysis of the businesses awareness level in the Baltic Sea Region countries
2018
Circular economy is a model of production and consumption, which involves sharing, leasing, reusing, repairing, refurbishing and recycling existing materials and products as long as possible. In this way, the life cycle of products is extended. Unlike the traditional linear economic model based on a ‘take-make-consume-throw away’ pattern, a circular economy is based on the notion that all wastes can be considered as valuable resources in an almost closed loop, where products and the materials they contain are highly valued. In practice, it implies reducing waste to a minimum. Examination of the current knowledge and performance on resource efficiency and circular economy of industrial enter…
Combination of acoustic levitation with small angle scattering techniques and synchrotron radiation circular dichroism. Application to the study of p…
2016
Abstract Background The acoustic levitation technique is a useful sample handling method for small solid and liquids samples, suspended in air by means of an ultrasonic field. This method was previously used at synchrotron sources for studying pharmaceutical liquids and protein solutions using x-ray diffraction and small angle x-ray scattering (SAXS). Methods In this work we combined for the first time this containerless method with small angle neutron scattering (SANS) and synchrotron radiation circular dichroism (SRCD) to study the structural behavior of proteins in solutions during the water evaporation. SANS results are also compared with SAXS experiments. Results The aggregation behavi…