Search results for "circulating"

Screening of microalgae and LED grow light spectra for effective removal of dissolved nutrients from cold-water recirculating aquaculture system (RAS…

2019

Popularity of recirculating aquaculture systems (RAS) is increasing. Because of the high water recirculation rate, dissolved nutrients originating from fish feed are concentrated enough in RAS wastewater (WW) to enable growth of primary producers, e.g. microalgae. This study evaluated nitrate-nitrogen (NO3-N) and phosphate-phosphorus (PO4-P) removal efficiency of ten temperate zone freshwater microalgae species during their exponential growth phase in unfiltered RAS WW at 17 ± 0.5 °C. Growth and nutrient uptake efficiency of six green and four non-green microalgae strains were compared between WW and reference growth medium in batch monocultures. The effect of three different LED grow light…

Catalyzing transcriptomics research in cardiovascular disease: The CardioRNA COST action CA17129

2019

WOS: 000474931400001

Establishing PNB-qPCR for quantifying minimal ctDNA concentrations during tumour resection.

2017

The analysis of blood plasma or serum as a non-invasive alternative to tissue biopsies is a much-pursued goal in cancer research. Various methods and approaches have been presented to determine a patient’s tumour status, chances of survival, and response to therapy from serum or plasma samples. We established PNB-qPCR (Pooled, Nested, WT-Blocking qPCR), a highly specific nested qPCR with various modifications to detect and quantify minute amounts of circulating tumour DNA (ctDNA) from very limited blood plasma samples. PNB-qPCR is a nested qPCR technique combining ARMS primers, blocking primers, LNA probes, and pooling of multiple first round products for sensitive quantification of the sev…

P2.01-017 Circulating miRNAs in Lung Cancer Are Associated to Pro-Tumorigenic and Immunosuppressive Microenvironment

2017

Filtration of Nordic recirculating aquaculture system wastewater : Effects on microalgal growth, nutrient removal, and nutritional value

2021

Microalgal bioremediation of recirculating aquaculture system (RAS) wastewater represents an alternative for wastewater treatment with the potential to generate valuable biomass. This study evaluated the effects of removing biological contamination and suspended solids from Nordic area RAS wastewater through filtration with 0.45 μm filters on the performance and nutritional value of microalgae. All three tested green microalgae (Haematococcus pluvialis, Monoraphidium griffithii, and Selenastrum sp.) were able to grow in raw (unfiltered) and filtered RAS wastewater. Cultivation in raw RAS wastewater decreased the ω-3 and ω-6 fatty acid content of H. pluvialis as compared to filtered wastewat…

Next-Generation Sequencing in Clinical Practice

2019

Abstract During the past few decades, Sanger sequencing represented the “gold standard” technique. In order to better define the mutational status of several genes at the same time, next-generation sequencing methodologies have been introduced in the molecular laboratory workflow. In the era of personalized medicine, this technological improvement plays a key role in the comprehensive molecular characterization of cancer patients in order to get a “tile” target therapy. For different cancer patients, different target therapies are available and different genes serve as clinical it relevant biomarkers. This chapter reviews the principal features of these novel technologies and their applicat…

Tumor markers in urogynaecological tumors.

2005

Circulating tumor and immune cells for minimally invasive risk stratification of smoldering multiple myeloma

2022

Abstract Purpose: Early intervention in smoldering multiple myeloma (SMM) requires optimal risk stratification to avoid under- and overtreatment. We hypothesized that replacing bone marrow (BM) plasma cells (PC) for circulating tumor cells (CTC), and adding immune biomarkers in peripheral blood (PB) for the identification of patients at risk of progression due to lost immune surveillance, could improve the International Myeloma Working Group 20/2/20 model. Experimental Design: We report the outcomes of 150 patients with SMM enrolled in the iMMunocell study, in which serial assessment of tumor and immune cells in PB was performed every 6 months for a period of 3 years since enrollment. Resul…

The molecular profiling of solid tumors by liquid biopsy: a position paper of the AIOM–SIAPEC-IAP–SIBioC–SIC–SIF Italian Scientific Societies

2021

The term liquid biopsy (LB) refers to the use of various biological fluids as a surrogate for neoplastic tissue to achieve information for diagnostic, prognostic and predictive purposes. In the current clinical practice, LB is used for the identification of driver mutations in circulating tumor DNA derived from both tumor tissue and circulating neoplastic cells. As suggested by a growing body of evidence, however, there are several clinical settings where biological samples other than tissue could be used in the routine practice to identify potentially predictive biomarkers of either response or resistance to targeted treatments. New applications are emerging as useful clinical tools, and o…

Filtration of Nordic recirculating aquaculture system wastewater: Effects on microalgal growth, nutrient removal, and nutritional value

2021

Abstract Microalgal bioremediation of recirculating aquaculture system (RAS) wastewater represents an alternative for wastewater treatment with the potential to generate valuable biomass. This study evaluated the effects of removing biological contamination and suspended solids from Nordic area RAS wastewater through filtration with 0.45 μm filters on the performance and nutritional value of microalgae. All three tested green microalgae (Haematococcus pluvialis, Monoraphidium griffithii, and Selenastrum sp.) were able to grow in raw (unfiltered) and filtered RAS wastewater. Cultivation in raw RAS wastewater decreased the ω-3 and ω-6 fatty acid content of H. pluvialis as compared to filtered…