Search results for "citru"
showing 10 items of 404 documents
Granulocyte functions are independent of arginine availability.
2014
Abstract Arginine depletion via myeloid cell arginase is critically involved in suppression of the adaptive immune system during cancer or chronic inflammation. On the other hand, arginine depletion is being developed as a novel anti-tumor metabolic strategy to deprive arginine-auxotrophic cancer cells of this amino acid. In human immune cells, arginase is mainly expressed constitutively in PMNs. We therefore purified human primary PMNs from healthy donors and analyzed PMN function as the main innate effector cell and arginase producer in the context of arginine deficiency. We demonstrate that human PMN viability, activation-induced IL-8 synthesis, chemotaxis, phagocytosis, generation of RO…
Activation of nitric oxide signaling by the rheumatoid arthritis shared epitope
2006
Objective. Susceptibility to rheumatoid arthritis (RA) is closely associated with HLA–DRB1 alleles encoding a shared epitope (SE) in positions 70–74 of the HLA–DR chain. The mechanistic basis for this association is unknown. Given the proposed pathogenic role of nitric oxide (NO) in RA, this study was undertaken to examine whether the SE can trigger NO signaling events. Methods. The intracellular levels of NO were measured with the fluorescent NO probe 4,5diaminofluorescein diacetate and by the 2,3diaminonaphthalene method. NO synthase activity was determined by measuring the rate of conversion of radioactive arginine to citrulline. Levels of cGMP were measured with a commercial enzyme-link…
Proteomic analysis of Kveim reagent identifies targets of cellular immunity in sarcoidosis
2017
Background Kveim-reagent (Kv) skin testing was a historical method of diagnosing sarcoidosis. Intradermal injection of treated sarcoidosis spleen tissue resulted in a granuloma response at injection site by 4–6 weeks. Previous work indicates proteins as the possible trigger of this reaction. We aimed to identify Kv-specific proteins and characterise the ex vivo response of Peripheral Blood Mononuclear Cells (PBMCs) from sarcoidosis, tuberculosis and healthy control patients when stimulated with both Kv and selected Kv-specific proteins. Methods Kv extracts were separated by 1D-SDS-PAGE and 2D-DIGE and then underwent mass spectrometric analysis for protein identification. Sarcoidosis and con…
Optimization of acetate production from citrus wastewater fermentation
2021
Abstract Citrus wastewater is a sugar-rich waste stream suitable for the recovery of energy of material from its treatment. In this study, fermentation of citrus wastewater was carried out to assess the optimal conditions to maximize the bioconversion of the organic substrate into acetate. Unbalanced nutrient (C: N: P 200:0.1:0.1) enabled the highest acetate production. The presence of the particulate organic fraction enabled to obtain a higher acetate concentration regardless the initial COD concentration. Initial pH values higher than 5 did not cause substantial differences on the maximum bioconversion of COD into acetate in Trial 3, whereas pH lower than 5 hindered the hydrolysis process…
Organic residues in lake and drinking water from the lagoa de Juturnaíba (região dos lagos - RJ, Brazil)
1997
Various analytical methods for the determination of organic residues in water were applied to samples from the Lagoa de Juturnaíba in the Região dos Lagos, east of Rio de Janeiro. The concentrations of more than 60 pesticides and of different aminopolycarboxylic acids were determined using GC/MS detection, and a GC/MS screening procedure was applied to detect additional compounds. None of the investigated pesticide residues could be detected in the lake water at detection limits of less than 100 ng/L. EDTA was detected at a concentration level of 2 mg/L in the lake and in drinking water prepared from this lake. Traces of biphenyl were identified by GC/MS in the lake water, the source of whi…
Effect of Fibre-Enriched Orange Juice on Postprandial Glycaemic Response and Satiety in Healthy Individuals: An Acute, Randomised, Placebo-Controlled…
2019
Background: Consumption of fibre-enriched orange juice may be an appropriate way to supplement daily fibre intake and achieve beneficial effects on metabolic health. The present study aimed to assess the short-term effects of fibre-enriched orange juice on postprandial metabolism and satiety in a healthy adult population. Methods: In this double-blind, randomised, placebo-controlled, crossover study 10 healthy subjects underwent two one-day trials in which they consumed an orange juice beverage containing 1.4 g/100 mL of citrus fibre (29.3% soluble and 41.9% insoluble) or a placebo (regular orange juice without added fibre). Postprandial glucose, insulin, gut hormones (GLP1, GIP and ghrelin…
Potential involvement of IL-9 and Th9 cells in the pathogenesis of rheumatoid arthritis
2015
Objective IL-9 has been shown to be upregulated before the clinical onset of articular disease in RA. The exact role of IL-9 and Th9 cells in RA, however, has not yet been adequately studied. The aim of this study was to evaluate the expression of IL-9 and IL-9-expressing cells in RA patients. Methods IL-9, IL-9R, PU.1, IL-9, thymic stromal lymphopoietin (TSLP), IL-4 and TGF-β expression was assessed by real-time-PCR in the synovial tissues of RA and OA patients. IL-9, IL-9R, IL-4, TSLP and TGF-β were also investigated by immunohistochemistry. Peripheral CD4(+) T cell subsets were studied by flow cytometry analysis before and after incubation with citrullinated peptides. Results IL-9 was ov…