Search results for "clone"
showing 10 items of 312 documents
Augmented antigen presentation by mouse Ia + T clone cells BK-BI-2.6.O4.1 mediated by transferrin receptors.
1996
The murine T clone cells BK-BI-2.6.O4.1 (BI/O4.1) synthesize and express MHC class II molecules constitutively. BI/O4.1 cells are able to present various protein antigens to antigen-specific CD4 + T cells. However, a 10-fold higher concentration of antigen is needed to activate specific T cells to lymphokine secretion by BI/O4.1 cells in comparison with spleen cells or with the more homogeneous population of bone marrow-derived macrophages (BMMph). The authors tested whether the reduced antigen presentation potential of BI/O4.1 cells was augmented by transferrin-mediated uptake of the model antigen ovalbumin (OVA) coupled to human ferric transferrin. It was shown that 240-fold less OVA was …
H2O2 resistant mesoangioblast clone isolation with a distinct survival advantage in vitro and in vivo
2017
The release of molecules from damaged tissues stimulates both resident and circulating stem cells to initiate a tissue repair programme. 1 However, during transplantation procedures the therapeutic efficacy of stem cells is compromised by reduced homing capability towards the target site.2,3 Furthermore, cell survival is very low and many studies focused on improving cell viability upon implantation. In this study, we performed in vitrosevere oxidative stress to select some more resistant mouse mesoangioblasts, vessel-associated progenitor stem cells endowed with the ability of multipotent mesoderm differentiation. We found that the selected subpopulation retains selfrenewal and myogenic di…
Immunoreactivity using anti-ERG monoclonal antibodies in sarcomas is influenced by clone selection.
2014
The aim of the present study was to explore ERG immunoreactivity in a series of sarcomas, GIST and malignant rhabdoid tumor (MRT), considering the not fully elucidated specificity and sensitivity of this antibody. Paraffin-embedded tissue microarrays from those tumors were stained with anti-ERG against the C-terminus [(EPR3864(2)] and N-terminus (Clone 9FY). EPR3864(2) was positive in almost all angiosarcomas, and MRT.GIST were positive in a large proportion of cases (38.4%), and more than half the synovial sarcomas (52.7%) revealed EPR3864(2) staining. Several chondrosarcomas, osteosarcomas, rhabdomyosarcoma and Ewing's sarcoma family of tumors (ESFT) presented EPR3864(2) expression in a l…
The intraclonal and interclonal phenotypic heterogeneity in a rhabdomyosarcoma cell line with abortive imitation of embryonic myogenesis
1988
Three distinct subpopulations (A, B, C) derived from a dimethylbenzanthracene-induced rat rhabdomyosarcoma were established as permanent cell lines. Although the clonal nature of each of these subpopulations was confirmed by repeated recloning procedures, a striking intraclonal phenotypic heterogeneity was observed. By means of immunofluorescence microscopy and transmission electron microscopy, it could be shown that these subpopulations closely recapitulate stages of embryonic rhabdomyogenesis both in vitro and in vivo, but differ in their particular range of maximum differentiation. Embryonic rhabdomyogenesis is imitated most perfectly by subpopulation C, in which multinuclear myotubes ar…
Identification of high-affinity phage-displayed VH fragments by use of a quartz crystal microbalance with dissipation monitoring
2021
10 Pág. Departamento de Tecnología de Alimentos
The Static Stability of the Tropopause Region in Adiabatic Baroclinic Life Cycle Experiments
2011
Abstract The tropopause inversion layer (TIL) is a region of enhanced static stability just above the WMO-defined thermal tropopause. It is a ubiquitous feature in midlatitudes and is well characterized by observations. However, it is still lacking a satisfactory theoretical explanation. This study utilizes adiabatic baroclinic life cycle experiments to investigate dynamical mechanisms that lead to TIL formation. As the baroclinic wave grows, a strong TIL forms above anticyclonic anomalies, while no TIL is found above cyclonic anomalies; this is consistent with previous results. However, during the early growth phase there is no TIL in the global or zonal average: positive and negative anom…
Evolution of fitness in experimental populations of vesicular stomatitis virus
1996
Abstract The evolution of fitness in experimental clonal populations of vesicular stomatitis virus (VSV) has been compared under different genetic (fitness of initial clone) and demographic (population dynamics) regimes. In spite of the high genetic heterogeneity among replicates within experiments, there is a clear effect of population dynamics on the evolution of fitness. Those populations that went through strong periodic bottlenecks showed a decreased fitness in competition experiments with wild type. Conversely, mutant populations that were transferred under the dynamics of continuous population expansions increased their fitness when compared with the same wild type. The magnitude of …
Future changes in North Atlantic winter cyclones in CESM-LENS. Part I: cyclone intensity, PV anomalies and horizontal wind speed
2021
Abstract. Strong low-level winds associated with extratropical cyclones can cause substantial impacts on society. The wind intensity and the spatial distribution of wind maxima may change in a warming climate; however, the involved changes in cyclone structure and dynamics are unclear. Here, such structural changes of strong North Atlantic cyclones in a warmer climate close to the end of the current century are investigated with storm-relative composites based on Community Earth System Model Large Ensemble (CESM-LENS) simulations. Furthermore, a piecewise potential vorticity inversion is applied to associate such changes in low-level winds to changes in potential vorticity (PV) anomalies at…
Variability of ecdysteroid-induced cell cycle alterations in Drosophila Kc sublines.
1987
. The cell cycle of two lines isolated from Drosophila Kc cells was followed by flow cytofluorometry and cell counting. The first line is the 8-9K clone which grew in a medium supplemented with 5% serum; the second, named subline Kc0, grew in a serum-free medium. The stationary phase is characterized by a G2 cell accumulation: 73% in the 8-9K clone and 50% in the Kc0 subline. When the medium was supplemented with the steroid moulting hormone 20-hydroxyecdysone, more than 90% of 8-9K cells and 65% of Kc0 cells were progressively arrested in G2. In the continuous presence of 20-hydroxyecdysone, most of the 8-9K cells remain G2-arrested; no massive G2 release into M was observed and only a few…
Processing requirements for the recognition of insulin fragments by murine T cells.
1988
In this study we investigated aspects of antigen processing using insulin and insulin A chain-derived fragments as model antigens in Ab alpha Ak beta-restricted T-cell stimulation. Similarly to other proteins, the immunodominant region of insulin recognized by these T cells is limited in size. It is located on the insulin A chain and encompasses a portion of the molecule that is represented faithfully by peptide A1-14(SSO3-)3. Efficient presentation of intact insulin and its entire A chain is dependent on uptake and processing by APC. Whereas peptides stemming from various globular proteins are known to be presented to T cells by APC without requiring processing, this is not the case with A…