Search results for "clone"
showing 10 items of 312 documents
Caracterización y tipificación molecular de cepas de Escherichia coli y Klebsiella pneumoniae productoras de betalactamasas de espectro extendido (BL…
2021
Introducción: Las betalactamasas de espectro extendido (BLEE) son enzimas producidas principalmente por la familia Enterobacteriaceae y se caracterizan porque inactivan a la práctica totalidad de los antibióticos betalactámicos. El aislamiento clínico de cepas productoras de BLEE es cada vez más frecuente en todo el mundo. Diferentes especies de Enterobacterales pueden ser productoras de BLEE, pero Klebsiella pneumoniae y Escherichia coli son las especies que las producen mayoritariamente. La realización de estudios de caracterización epidemiológica de las cepas productoras de BLEE es fundamental para detectar cualquier cambio en la resistencia y por tanto, para el control de su diseminació…
Community Resilience to Cyclone Disasters in Coastal Bangladesh
2016
Bangladesh is one of the poorest and most disaster-prone countries in the world. To address both problems simultaneously, sustainable livelihoods (SL) could be better connected with disaster risk reduction (DRR). For this purpose, one initiative implemented in Bangladesh is called the Vulnerability to Resilience (V2R) programme which ran from 2013 to 2016. This programme was primarily initiated and funded by the British Red Cross in a consortium with the Swedish Red Cross and the German Red Cross. This article presents the first evaluation of the V2R programme with three objectives. The first objective was to measure whether the selected communities have achieved community resilience charac…
Responsible cognitive digital clones as decision-makers: A design science research study
2022
This study uses a design science research methodology to develop and evaluate the Pi-Mind agent, an information technology artefact that acts as a responsible, resilient, ubiquitous cognitive clone – or a digital copy – and an autonomous representative of a human decision-maker. Pi-Mind agents can learn the decision-making capabilities of their “donors” in a specific training environment based on generative adversarial networks. A trained clone can be used by a decision-maker as an additional resource for one’s own cognitive enhancement, as an autonomous representative, or even as a replacement when appropriate. The assumption regarding this approach is as follows: when someone was forced t…
Towards digital cognitive clones for the decision-makers: adversarial training experiments
2021
Abstract There can be many reasons for anyone to make a digital copy (clone) of own decision-making behavior. This enables virtual presence of a professional decision-maker simultaneously in many places and processes of Industry 4.0. Such clone can be used as one’s responsible representative when the human is not available. Pi-Mind (“Patented Intelligence”) is a technology, which enables “cloning” cognitive skills of humans using adversarial machine learning. In this paper, we present a cyber-physical environment as an adversarial learning ecosystem for cloning image classification skills. The physical component of the environment is provided by the logistic laboratory with camera-surveilla…
New particle formation inside ice clouds: In-situ observations in the tropical tropopause layer of the 2017 Asian Monsoon Anticyclone
2021
Abstract. From 27 July to 10 August 2017 the airborne StratoClim mission took place in Kathmandu, Nepal where eight mission flights were conducted with the M-55 Geophysica up to altitudes of 20 km. New Particle Formation (NPF) was identified by the abundant presence of ultrafine aerosols, with particle diameters dp smaller than 15 nm, which were in-situ detected by means of condensation nuclei counting techniques. NPF fields in clear-skies as well as in the presence of cloud ice particles (dp > 3 µm) were encountered at upper troposphere/lowermost stratosphere (UT/LS) levels and within the Asian Monsoon Anticyclone (AMA). NPF-generated ultrafine particles in elevated concentrations (Nuf)…
Analysis of Multilocus Sequence Typing and Virulence Characterization of Listeria monocytogenes Isolates from Chinese Retail Ready-to-Eat Food
2016
Eighty Listeria monocytogenes isolates were obtained from Chinese retail ready-to-eat (RTE) food and were previously characterized with serotyping and antibiotic susceptibility tests. The aim of this study was to characterize the subtype and virulence potential of these L. monocytogenes isolates by multilocus sequence typing (MLST), virulence-associate genes, epidemic clones (ECs), and sequence analysis of the important virulence factor: internalin A (inlA). The result of MLST revealed that these L. monocytogenes isolates belonged to 14 different sequence types (STs). With the exception of four new STs (ST804, ST805, ST806, and ST807), all other STs observed in this study have been associat…
Genetic diversity of a clonal earthworm : gene expression variation and impacts on decomposition in metal contaminated soil
2016
Biodiversity affects ecosystem functioning positively. Metal-contamination diminishes species diversity and ecosystem function depends on the remaining species, and the genotypes within them. I studied how the clonally reproducing earthworm Dendrobaena octaedra responds to soil metal contamination and how its genetic diversity affects decomposition. Expression of the gene for the metal tolerance protein metallothionein was compared between populations with or without metal exposure history: with exposure history expression was high and stable and without there was a slow response. Adaptation to metal contamination likely explains the differences. Clonality of D. octaedra was verified with m…
Lymphocytes from hepatic inflammatory infiltrate kill rat hepatocytes in primary culture
1990
In the last few years it has become possible in the liver to isolate lymphocytes from inflammatory infiltrates and to culture them in vitro. Most of the lymphocyte clones obtained are CD 8 + cytotoxic cells, but interactions between these lymphocytes and hepatocytes in primary culture have not been analysed previously. In this study, cloned human T lymphocytes from liver biopsies and from the peripheral blood of patients with chronic hepatitis B or primary biliary cirrhosis, after phenotypical and functional characterization into CD 8+ or CD 4+ cytotoxic lymphocytes, were activated in an antigen-independent fashion by adding either anti CD 3 or anti CD 2/R-3 monoclonal antibodies to the cel…
Spatial and temporal variations in cuticle proteins as revealed by monoclonal antibodies. Immunoblotting analysis and ultrastructural immunolocalizat…
1989
A library of monoclonal antibodies (Mabs) against adult cuticle of Tenebrio was used to visualize the secretion of cuticular antigens during metamorphosis. Immunoblots of water- and urea-soluble proteins, and high resolution immunogold labelling has shown that, except in one clone, the Mabs recognize antigens in the three developmental stages. However, the MW of larval and pupal antigens are different from the adult ones, though sharing common epitopes. Blots of cuticle proteins (CPs) bound to different lectins shown few water-soluble glycosylated proteins weakly or not recognized by the Mabs, suggesting that the majority of the Mabs do not recognize glycosylated epitopes. The immunolocaliz…
IFN-alpha Stimulates Proliferation and Cytokine Secretion of CD40-Stimulated B Cell Chronic Lymphocytic Leukemia Cells In Vitro
1999
Interferon (IFN)-alpha has a therapeutic effect in several B cell malignancies, including low-grade non-Hodgkin's lymphoma (NHL), multiple myeloma, and hairy cell leukemia, whereas its efficacy in the treatment of B cell chronic lymphocytic leukemia (B-CLL) is rather limited. In the present study, we investigated the effect of IFN-alpha on the biologic functions of B-CLL cells, which were stimulated by cross-linking of the CD40 antigen. In cell samples from 16 B-CLL patients, the addition of IFN-alpha to CD40-stimulated purified B-CLL cells caused a significant increase in [3H]thymidine uptake (p < 0.003). In B-CLL cells maximally activated by CD40 cross-linking and interleukin-2 (IL-2)/IL-…