Search results for "complex"
showing 10 items of 5889 documents
Quantification of metal-resistant genes from the microbial community of uranium mine soils
2019
Background: Bacteria can thrive in environments presenting unique, extreme and/or stressful conditions, partly due to the diverse resistome they evolved as a result of physiological, metabolic and genetic adaptations. In this context, the microbial communities in soils from abandoned uranium mines may have peculiar genetic profiles that sustain valuable metal-resistance mechanisms. Getting further knowledge on the genetic potential of these microbial communities can be a contribute for developing bioremediation strategies towards the reclamation of highly contaminated uranium mine soils. Objectives: To quantify metal-resistance genes harboured by the microbial community of soil samples coll…
Characterization of the biosynthesis of saponins during seed development in peas (Pisum sativum)
2019
National audience; The use of pulses as ingredients for the production of food products rich in plant proteins is increasing. However, protein fractions prepared from pea or other pulses contain significant amounts of saponins, glycosylated triterpenes which can impart a bitter taste to the final food product. Bitter flavours are currently either removed by energy-requiring physico-chemical treatments or masked by additives. We are in the process of identifying and characterizing the genes involved in saponin biosynthesis during pea seed development, with the objective of identifying mutants in which seed saponins no longer accumulate. To do this we have applied a saponin extraction protoco…
Understanding the microbiological and physicochemical influence of alperujo vermicompost on diuron behaviour and soil quality
2009
Vermicompost as soil organic amendment increase the yield crops because improve the soil structure, the water retention capacity, the microbial biodiversity and thereby its metabolic activity. Vermicompost is also thought to reduce the pesticide pollution. The vermicompost selected was composed of “alperujo” which is largest organic residue in olive oil production. Agricultural soil was 5% amended twice and incubated for 3 months. A 3µg g-1 agricultural dosage of Diuron was applied to amended and unamended soils. The effect of alperujo vermicompost on dehydrogenase activity, the biodiversity and estimated bacterial density, the density of protocathechuate-degrading bacterial community and d…
Immobilization of murine noroviruses in a cultivated phaeozem soil and its reversibility
2017
Immobilization of murine noroviruses in a cultivated phaeozem soil and its reversibility. Environmental Microbiology & Microbial Ecology
Diversity and complexity of microbial communities from a chlor-alkali tailings dump
2015
Abstract Revegetation of the tailings dumps produced by various industrial activities is necessary to prevent dust storms and erosion and represents a great challenge for ecological restoration. Little is known about the microbial colonisation and community structure of revegetated tailings following site exploitation. Here, we report the sequencing of 16S rRNA and internal transcribed spacer (ITS) fungal RNA gene amplicons from chlor-alkali residue and from an adjacent undisturbed soil to define the composition and assembly of the rhizosphere microbial communities. After quality filtering, a total of 72,373 and 89,929 bacterial sequences and 122,618 and 111,209 fungal sequences remained fo…
New molecular aspects of regulation of mitochondrial activity by fenofibrate and fasting
2000
Abstract Fenofibrate and fasting are known to regulate several genes involved in lipid metabolism in a similar way. In this study measuring several mitochondrial enzyme activities, we demonstrate that, in contrast to citrate synthase and complex II, cytochrome c oxidase (COX) is a specific target of these two treatments. In mouse liver organelles, Western blot experiments indicated that mitochondrial levels of p43, a mitochondrial T3 receptor, and mitochondrial peroxisome proliferator activated receptor (mt-PPAR), previously described as a dimeric partner of p43 in the organelle, are increased by both fenofibrate and fasting. In addition, in PPARα-deficient mice, this influence was abolishe…
Engineering a Saccharomyces cerevisiae Wine Yeast That Exhibits Reduced Ethanol Production during Fermentation under Controlled Microoxygenation Cond…
2006
ABSTRACTWe recently showed that expressing an H2O-NADH oxidase inSaccharomyces cerevisiaedrastically reduces the intracellular NADH concentration and substantially alters the distribution of metabolic fluxes in the cell. Although the engineered strain produces a reduced amount of ethanol, a high level of acetaldehyde accumulates early in the process (1 g/liter), impairing growth and fermentation performance. To overcome these undesirable effects, we carried out a comprehensive analysis of the impact of oxygen on the metabolic network of the same NADH oxidase-expressing strain. While reducing the oxygen transfer rate led to a gradual recovery of the growth and fermentation performance, its i…
Eradication of Plasmodiophora brassicae during composting of wastes
2006
Survival of infectious inoculum of the clubroot pathogen Plasmodiophora brassicae was assessed following bench-scale flask composting experiments and large-scale composting procedures. Clubroot-affected material was provided by artificial inoculation of Chinese cabbage or naturally infected Brussels sprout and cabbage roots. Both sets of diseased material were used in flask experiments, and the latter in large-scale windrow and aerated tunnel experiments. Municipal green wastes, onion waste and spent mushroom compost were evaluated in flask experiments with varying temperature, aeration and moisture conditions. Green wastes were used in larger-scale composts. Within the limits of a Chinese …
Une sépulture à incinérations multiples du Bronze final au pied d'un mégalithe à Epoigny (Couches, Saône-et-Loire): Observations sur quelques ensembl…
2006
National audience; Présentation d'une sépulture du bronze final au pied d'un mégalithe en Saône-et-Loire
Enfance et société : du complexe de Prométhée à l'enfance archétypale
2007
National audience