Search results for "corpi"
showing 10 items of 44 documents
Molecular Rearrangement of an Aza-Scorpiand Macrocycle Induced by pH: A Computational Study †
2016
Rearrangements and their control are a hot topic in supramolecular chemistry due to the possibilities that these phenomena open in the design of synthetic receptors and molecular machines. Macrocycle aza-scorpiands constitute an interesting system that can reorganize their spatial structure depending on pH variations or the presence of metal cations. In this study, the relative stabilities of these conformations were predicted computationally by semi-empirical and density functional theory approximations, and the reorganization from closed to open conformations was simulated by using the Monte Carlo multiple minimum method Financial support by the Spanish Ministerio de Economía y Competitiv…
Structural Mechanism of SDS-Induced Enzyme Activity of Scorpion Hemocyanin Revealed by Electron Cryomicroscopy
2009
Summary Phenoloxidases (POs) occur in all organisms and are involved in skin and hair coloring in mammals, and initiating melanization in wound healing. Mutation or overexpression of PO can cause albinism or melanoma, respectively. SDS can convert inactive PO and the oxygen carrier hemocyanin (Hc) into enzymatically active PO. Here we present single-particle cryo-EM maps at subnanometer resolution and pseudoatomic models of the 24-oligomeric Hc from scorpion Pandinus imperator in resting and SDS-activated states. Our structural analyses led to a plausible mechanism of Hc enzyme PO activation: upon SDS activation, the intrinsically flexible Hc domain I twists away from domains II and III in …
Crystallization and Preliminary Analysis of Crystals of the 24-Meric Hemocyanin of the Emperor Scorpion (Pandinus imperator)
2011
Hemocyanins are giant oxygen transport proteins found in the hemolymph of several invertebrate phyla. They constitute giant multimeric molecules whose size range up to that of cell organelles such as ribosomes or even small viruses. Oxygen is reversibly bound by hemocyanins at binuclear copper centers. Subunit interactions within the multisubunit hemocyanin complex lead to diverse allosteric effects such as the highest cooperativity for oxygen binding found in nature. Crystal structures of a native hemocyanin oligomer larger than a hexameric substructure have not been published until now. We report for the first time growth and preliminary analysis of crystals of the 24-meric hemocyanin (M(…
The Pandinus imperator haemolymph lipoprotein, an unusual phosphatidylserine carrying lipoprotein.
2009
The haemolymph lipoprotein of the scorpion, Pandinus imperator was isolated and characterised. Contrary to the lipoproteins of insects and the discoidal HDL-lipoproteins of a crayfish and polychaete, the Pandinus lipoprotein consists of three instead of two apoproteins (apoPiLp I = 230 kDa, apoPiLp II = 130 kDa and apoPiLp III = 120 kDa). The apolipoproteins are arranged in varying stoichiometries as judged by cross-linking experiments. In lipoprotein samples from individual animals, the two smaller subunits occurred in a 1:1 stoichiometry, while the relative amount of the 230 kDa peptide varied. The lipoprotein is a slightly heart-shaped HDL with a diameter of approximately 15 nm. It is pr…
Spectral Evolution of Scorpio X‐1 along its Color‐Color Diagram
2007
We analyze a large collection of RXTE archive data of the bright X‐ray source Scorpius X‐1 in order to study the broadband spectral evolution of the source for different values of the inferred mass accretion rate by selecting energy spectra from its Color‐Color Diagram. We model the spectra with the combination of two absorbed components: a soft thermal component, which can be interpreted as thermal emission from an accretion disk, and a hybrid Comptonization component, which self‐consistently includes the Fe Kα fluorescence line and the Compton reflected continuum. The presence of hard emission in Scorpius X‐1 has been previously reported, however, without a clear relation with the accreti…
Fe K α and Fe K β line detection in the NuSTAR spectrum of the ultra-bright Z source Scorpius X-1
2021
Context.Low-mass X-ray binaries hosting a low-magnetised neutron star, which accretes matter via Roche-lobe overflow, are generally grouped into two classes called Atoll and Z sources after the path described in their X-ray colour-colour diagrams. Scorpius X–1 is the brightest persistent low-mass X-ray binary known so far, and it is the prototype of the Z sources.Aims.We analysed the firstNuSTARobservation of this source to study its spectral emission, exploiting the high-statistics data collected by this satellite. The colour-colour diagram shows that the source was probably observed during the lower normal and flaring branches of its Z track. We separated the data from the two branches in…
Metodo e kit per la sclerosi multipla
2011
E’ descritta la scoperta di anticorpi anti-transferrina come biomarcatori per la sclerosi multipla da cui è stato messo a punto un metodo di diagnosi, prognosi, monitoraggio della terapia e relativo kit.