Search results for "crisp"
showing 10 items of 104 documents
The role of the transcription factor hsf1 in zebrafish erythropoiesis
2020
Heat shock proteins (HSPs) are reported to play an important role in erythropoiesis. The expression of HSP genes is mainly controlled by Heat shock factor 1 (HSF1), a highly conserved transcription factor. So far, a detailed understanding of the function of HSF1 in erythropoiesis remains uncharacterized. This study has employed zebrafish as a relevant model to investigate the role of Hsf1 during embryonic erythropoiesis. We established hsf1-disrupted zebrafish lines using the CRISPR/Cas9 technology and performed phenotypic analyses throughout embryogenesis. We found that Hsf1 deficient embryos had a decreased number of primitive erythrocytes, while erythrocyte number in adults was unchanged…
FRANCESCO CRISPI DAL DISCENTRAMENTO AMMINISTRATIVO ALLA PROPOSTA DI ISTITUZIONE DELLE CIRCOSCRIZIONI AMMINISTRATIVE REGIONALI
2021
Questo contributo trae spunto dalla relazione tenuta a Roma, il 24 settembre 2018, insieme al Prof. Franco Gaetano Scoca, al Prof. Francesco Bonomi, al Prof. Guido Melis e al Prof. Marcello Saija, presso la Camera dei deputati nella IV sessione del Convegno “Rileggiamo Crispi a 200 anni dalla nascita (1818-2018)”.
First record of Ribes uva-crispa L. (Grossulariaceae) from the Madonie Mts., a new species of the Sicilian flora.
2011
Abstract In this study, a first record of Ribes uva-crispa L. (Grossulariaceae) – a new species of the Sicilian flora – from the Madonie Mts. is reported. The autochthonous and relict new population of Ribes uva-crispa L. (Grossulariaceae) is found on Mount Carbonara (Madonie Mts., North-Central Sicily), which is several hundred kilometers away from the Central Apenninic and the South Mediterranean locations of its distribution range. R. uva-crispa shows a distribution similar to other taxa extending from the Euro-Asiatic area to the mountains of North Africa with disjunct populations on the main Sicilian reliefs (Madonie and Nebrodi Mts.), testifying ancient phytogeographical connections. …
Francesco Crispi giurista
2019
Il volume ripercorre la biografia di Francesco Crispi valutando i giudizi della storiografia alla luce delle recenti ricerche. Vengono qui superati gli schemi dell’incoerenza tra il giovane e il vecchio Crispi, per i quali lo si addita prima cospiratore repubblicano poi riformista monarchico, quindi rivoluzionario poi repressore, infine sostenitore dell’autodeterminazione dei popoli poi colonialista. I saggi rintracciano il filo conduttore di un uomo che sin dalle prime esperienze professionali e politiche mostra di credere nella costruzione modernizzante di uno Stato amministrativo capace di scardinare i residui notabiliari e di governare secondo un disegno, certo elitario, in linea con la…
Francesco Crispi le riforme amministrative. Il primo intervento di riforma strutturale dello Stato unitario
2023
Questo contributo analizza il rapporto tra le riforme propugnate dai Governi guidati da Francesco Crispi (ben quattro, tra il 1887 ed il 1891 e tra il 1894 ed 1896) ed il loro artefice, con uno sguardo d’insieme sul complesso processo di modernizzazione dell’Italia che si affacciava al “secolo breve” con un’organizzazione amministrativa arcaica. Partendo dalla formazione giuridica dello statista siciliano, dagli scritti giovanili, dall’attività professionale, dalla cultura risorgimentale e costituzionale che ne forgiarono la personalità e poi dall’attività rivoluzionaria e parlamentare, lo studio perlustra il variegato contesto nel quale proruppe quell’“impeto riformatore dell’amministrazio…
Midbiotics: conjugative plasmids for genetic engineering of natural gut flora.
2019
ABSTRACT The possibility to modify gut bacterial flora has become an important goal, and various approaches are used to achieve desirable communities. However, the genetic engineering of existing microbes in the gut, which are already compatible with the rest of the community and host immune system, has not received much attention. Here, we discuss and experimentally evaluate the possibility to use modified and mobilizable CRISPR-Cas9-endocing plasmid as a tool to induce changes in bacterial communities. This plasmid system (briefly midbiotic) is delivered from bacterial vector into target bacteria via conjugation. Compared to, for example, bacteriophage-based applications, the benefits of …
Proposal of a Genome Editing System for Genetic Resistance to Tomato Spotted Wilt Virus
2014
Viruses provoke considerable economical losses in agriculture. New molecular approaches to develop genetic resistance based on translational genomics and precision genetic modifications are highly expected. The type II Clustered, Regularly Interspaced Palindromic Repeats (CRISPR) system including Cas9 nuclease represent a promising and very powerful tool to specifically modulate the expression and activity of genes involved in biotic stress responses. In this study, we describe an approach to develop a platform system based on CRISPR system for genome editing technology in tomato. Tomato is an excellent plant for this approach considering the high-quality genome sequence, the rapid life cyc…
Hutchinson Gilford Progeria Syndrome: A Therapeutic Approach via Adenoviral Delivery of CRISPR/cas Genome Editing System
2015
Hutchinson-Gilford Progeria Syndrome (HGPS) is a rare human genetic disease caused by mutations in the LMNA gene. LMNA codes for structural components of the nuclear lamina. Alterations of nuclear lamina lead to a very variable class of diseases known as laminopathies. In detail, HGPS manifests a severe premature ageing phenotype due to the accumulation of a dominant negative form of lamin-A called progerin. With current treatments, the life expectancy of HGPS patients does not exceed their second decade. Death is usually due to cardiovascular complications. Recently, a new technology for mammals in vivo gene editing has been developed: the clustered regularly interspaced short palindromic …
In vitro genome editing using CRISPR/Cas9 to edit SERPINA1 PiZ mutation
2019
Introduction: The emergence some years ago of the CRISPR/Cas9 system allowed gene therapy to be specific, versatile, cheap and approachable to almost every laboratory. Due to these features, many different genetic diseases such as cystic fibrosis or β-thalassemia have been addressed in cellular models using the CRISPR/Cas9 genetic editing tool. Alpha-1 antytripsin deficiency (AATD) is a rare genetic condition that can provoke respiratory and hepatic symptoms. The Z allele of SERPINA1 gene is a well-characterised point mutation that can trigger the whole pathology. Henceforth, Z mutation is a suitable target for genetic edition using CRISPR/Cas9 in order to develop a gene therapy to treat AA…
Legionella pneumophila pangenome reveals strain-specific virulence factors
2010
Abstract Background Legionella pneumophila subsp. pneumophila is a gram-negative γ-Proteobacterium and the causative agent of Legionnaires' disease, a form of epidemic pneumonia. It has a water-related life cycle. In industrialized cities L. pneumophila is commonly encountered in refrigeration towers and water pipes. Infection is always via infected aerosols to humans. Although many efforts have been made to eradicate Legionella from buildings, it still contaminates the water systems. The town of Alcoy (Valencian Region, Spain) has had recurrent outbreaks since 1999. The strain "Alcoy 2300/99" is a particularly persistent and recurrent strain that was isolated during one of the most signifi…