Search results for "cromatina"

showing 7 items of 7 documents

Topoisomerase II regulates yeast genes with singular chromatin architectures

2013

Eukaryotic topoisomerase II (topo II) is the essential decatenase of newly replicated chromosomes and the main relaxase of nucleosomal DNA. Apart from these general tasks, topo II participates in more specialized functions. In mammals, topo IIa interacts with specific RNA polymerases and chromatin-remodeling complexes, whereas topo IIb regulates developmental genes in conjunction with chromatin remodeling and heterochromatin transitions. Here we show that in budding yeast, topo II regulates the expression of specific gene subsets. To uncover this, we carried out a genomic transcription run-on shortly after the thermal inactivation of topo II. We identified a modest number of genes not invol…

BioquímicaHeterochromatinADNSaccharomyces cerevisiaeGene Regulation Chromatin and EpigeneticsGenètica molecularChromatin remodelingHistonesCromatina03 medical and health sciencesGene Expression Regulation FungalGeneticsNucleosomeDNA FungalPromoter Regions GeneticTranscription factor030304 developmental biologyGenetics0303 health sciencesbiologyPolyamine transport030302 biochemistry & molecular biologyPromoterExpressió gènicaChromatinChromatinNucleosomesHistoneDNA Topoisomerases Type IIMutationbiology.proteinGenèticaTranscription FactorsNucleic Acids Research
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Estudio funcional de factores implicados en la transcripción y exportación de los RNAs en Saccharomyces cerevisiae

2014

La expresión génica en eucariotas ocurre a través del acoplamiento de diferentes procesos finamente regulados e interconectados en los que participan varios complejos multiprotéicos. Nuestro trabajo se centra en el estudio de las conexiones entre esos procesos que aseguran una correcta formación del ARN. La proteína Sus1 es un factor esencial para el correcto acoplamiento entre la transcripción y la exportación de los ARN mensajeros para su traducción en el citoplasma (1, 2). Sus1 es componente de 3 complejos diferentes: -TREX-2, asociado al complejo del poro nuclear y tiene funciones en el metabolismo del ARN mensajero, la exportación del mismo al citoplasma y en estabilidad genómica; -SAG…

Cromatina:CIENCIAS DE LA VIDA::Bioquímica [UNESCO]UNESCO::CIENCIAS DE LA VIDA::Biología molecularRNAUNESCO::CIENCIAS DE LA VIDA::BioquímicaTranscripciónLevadura:CIENCIAS DE LA VIDA::Biología molecular [UNESCO]
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ROLE OF THE ISWI CHROMATIN REMODELER FACTOR IN TISSUE REGENERATION

2014

Durante lo sviluppo degli organismi multicellulari, le modifiche epigenetiche della cromatina garantiscono l’attuazione dei programmi trascrizionali che guidano i processi di determinazione del destino cellulare. La presenza di modifiche epigenetiche della cromatina inoltre, assicura il mantenimento e la propagazione, nel corso delle divisioni mitotiche, di una memoria epigenetica cellulare. Tuttavia, sono note almeno due eccezioni a questo processo: la rigenerazione e la transdeterminazione Durante la rigenerazione tissutale, un fenomeno evolutivamente molto conservato, cellule già determinate affrontano cicli di proliferazione durante i quali possono riprogrammare la loro memoria epigenet…

CromatinaSettore BIO/10 - BiochimicaRigenerazioneEpigenetica
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Caratterizzazione funzionale e strutturale di un isolatore cromatinico localizzato tra i geni c-Myc e Pvt-1 umani

2008

Settore BIO/18 - Geneticac-Myccromatinaenhancer blockerisolatore.
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Chromatin modifiers and recombination factors promote a telomere fold-back structure, that is lost during replicative senescence.

2020

Telomeres have the ability to adopt a lariat conformation and hence, engage in long and short distance intra-chromosome interactions. Budding yeast telomeres were proposed to fold back into subtelomeric regions, but a robust assay to quantitatively characterize this structure has been lacking. Therefore, it is not well understood how the interactions between telomeres and non-telomeric regions are established and regulated. We employ a telomere chromosome conformation capture (Telo-3C) approach to directly analyze telomere folding and its maintenance in S. cerevisiae. We identify the histone modifiers Sir2, Sin3 and Set2 as critical regulators for telomere folding, which suggests that a dis…

TelomeraseProtein Folding:Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::DNA-Binding Proteins::Rad52 DNA Repair and Recombination Protein [Medical Subject Headings]:Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Fungal Proteins::Saccharomyces cerevisiae Proteins [Medical Subject Headings]Gene ExpressionYeast and Fungal ModelsArtificial Gene Amplification and ExtensionQH426-470BiochemistryPolymerase Chain ReactionChromosome conformation captureHistonesCromatina0302 clinical medicineSirtuin 2Macromolecular Structure AnalysisSilent Information Regulator Proteins Saccharomyces cerevisiaeCellular Senescence:Organisms::Eukaryota::Fungi::Yeasts::Saccharomyces::Saccharomyces cerevisiae [Medical Subject Headings]0303 health sciencesChromosome BiologyEukaryota:Phenomena and Processes::Genetic Phenomena::Genetic Processes::DNA Replication [Medical Subject Headings]TelomereSubtelomere:Anatomy::Cells::Cellular Structures::Intracellular Space::Cell Nucleus::Cell Nucleus Structures::Intranuclear Space::Chromosomes::Chromosome Structures::Telomere [Medical Subject Headings]Chromatin3. Good healthChromatinCell biologyNucleic acidsTelomeres:Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Cell Cycle::Cell Division::Telomere Homeostasis [Medical Subject Headings]Experimental Organism SystemsDaño del ADNEpigeneticsResearch ArticleSenescenceDNA Replication:Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Hydrolases::Amidohydrolases::Histone Deacetylases [Medical Subject Headings]Chromosome Structure and FunctionProtein StructureSaccharomyces cerevisiae ProteinsSaccharomyces cerevisiaeBiologyResearch and Analysis MethodsHistone DeacetylasesChromosomes03 medical and health sciencesSaccharomycesModel Organisms:Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Transferases::One-Carbon Group Transferases::Methyltransferases [Medical Subject Headings]:Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Intracellular Signaling Peptides and Proteins::Sirtuins::Sirtuin 2 [Medical Subject Headings]:Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Fungal Proteins::Saccharomyces cerevisiae Proteins::Silent Information Regulator Proteins Saccharomyces cerevisiae [Medical Subject Headings]DNA-binding proteinsGenetics:Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Recombinases::Rec A Recombinases::Rad51 Recombinase [Medical Subject Headings]Molecular Biology TechniquesMolecular Biology030304 developmental biologyCromosomasSenescencia celularOrganismsFungiBiology and Life SciencesProteinsTelomere HomeostasisCell BiologyDNAMethyltransferasesG2-M DNA damage checkpointProteína recombinante y reparadora de ADN Rad52YeastTelomereRad52 DNA Repair and Recombination ProteinRepressor ProteinsAnimal Studies:Chemicals and Drugs::Amino Acids Peptides and Proteins::Proteins::Transcription Factors::Repressor Proteins [Medical Subject Headings]DNA damageRad51 RecombinaseHomologous recombination030217 neurology & neurosurgeryTelómeroDNA DamagePLoS Genetics
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Analisi dell'espressione genica e dinamica della cromatinadel gene PITa2 in Paracentrotus Lividus.

2011

cromatina del gene PITa2Paracentrotus Lividus.Settore BIO/11 - Biologia Molecolare
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Determinación de marcas epigenéticas en genesimplicados en la respuesta temprana a Botrytis cinerea de Arabidopsis thaliana y Solanum lycopersicum

2018

Existen en estudios recientes realizados en plantas, evidencias que vinculan la presencia de modificaciones epigenéticas con su respuesta a estreses bióticos. Sin embargo, dentro de estos estreses no se encuentra a penas información sobre patógenos necrótrofos. En esta tesis se exploran las posibles alteraciones en la estructura cromatinica que plantas de las especies Arabidopsis thaliana y Solanum lycopersicum (tomate) podrían experimentar en varios de sus genes expresados en la respuesta a la infección por Botrytis cinerea, un hongo necrótrofo. Se plantea para ellos la puesta a punto de un protocolo de extracción de cromatina y de inmunoprecipitación de la misma (ChIP) y, se relatan las d…

cromatinaArabidopsis thaliana:QUÍMICA::Bioquímica ::Biología molecular [UNESCO]:CIENCIAS DE LA VIDA::Genética ::Genética molecular de plantas [UNESCO]UNESCO::CIENCIAS DE LA VIDA::Genética ::Genética molecular de plantasUNESCO::CIENCIAS DE LA VIDA::Biología molecular ::Biología molecular de plantasChIPinfección:CIENCIAS DE LA VIDA::Biología molecular ::Biología molecular de plantas [UNESCO]patógenoUNESCO::QUÍMICA::Bioquímica ::Biología molecularUNESCO::CIENCIAS AGRARIAS::Ciencias veterinarias ::Genética:CIENCIAS AGRARIAS::Ciencias veterinarias ::Genética [UNESCO]epigenéticaBotrytis cinereaSolanum lycopersicumestrés oxidativo
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