Search results for "cytokinin"
showing 2 items of 32 documents
Factors affecting adventitious bud induction in Pinus elliottii (Engelm.) embryos cultured in vitro
1987
Embryos of slash pine (Pinus elliottii Engelm.) were induced to form adventitious buds when placed in culture on nutrient media supplemented with cytokinin. Buds were induced on media containing Risser & White major salts. The high content in nitrogen of Murashige & Skoog formulation seems to be deleterious for this in vitro system, since morphogenic responses were only promoted when nitrogen concentration was drastically reduced in the macronutrient formulation. Factors such as concentration of cytokinin (6-benzyladenine) and time and method of exposure (liquid or solid induction medium) strongly influenced bud formation and development. The greatest number of buds and shoots were obtained…
In vitro morphogenesis of grapevine (Vitis vinifera L.) inflorescence primordia, cvs Pinot Noir and Chardonnay
1998
Hormonal regulatory processes controlling in vitro morphogenesis of grapevine inflorescence primordia, excised from latent buds from November to April, were investigated. Cytokinin (benzylaminopurine, BAP), gibberellin (GA3) and auxin (3-indolebutyric acid, IBA) were used at 1 mg/L. BAP induced branch development and flower calyx differentiation. GA3 first promoted the elongation of inflorescence rachis and then promoted flower differentiation up to the formation of normal calyx and calyptra (the fused petals), while anthers and pistils were also present but not functional. IBA did not seem to be directly involved in these morphogenetic processes. After culturing for 6–7 weeks, explants bec…