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showing 10 items of 8549 documents

Training session intensity affects plasma redox status in amateur rhythmic gymnasts

2016

Purpose: The aim of this study was to examine systemic responses of oxidant/antioxidant status following 2 training sessions of different intensity in amateur rhythmic gymnasts. Methods: Before the experimental training, 10 female gymnasts performed a gradually increased exercise test to assess maximal heart rate, maximal oxygen consumption, and anaerobic threshold. They executed 2 intermittent training sessions separated by 48 h of recovery (48 h-post R): the first was performed at low-moderate intensity (LMI) and the second at high intensity (HI). Blood samples were collected immediately pre- and post-training and 48 h-post R. Hydroperoxide level (OxL) and total antioxidant capacity (TAC)…

0301 basic medicineTraining intensitymedicine.medical_specialtyeducationPhysical Therapy Sports Therapy and RehabilitationFree radicalsmedicine.disease_cause03 medical and health scienceslcsh:GV557-1198.9950302 clinical medicineRhythmInternal medicineHeart rateRegular PapermedicineOrthopedics and Sports MedicineExercise physiologylcsh:Sports medicineAntioxidant capacity; Exercise physiology; Free radicals; Oxidative stress; Reactive oxygen species; Rhythmic gymnastics; Training intensitylcsh:SportsSettore M-EDF/02 - Metodi E Didattiche Delle Attivita' Sportivebusiness.industryRhythmic gymnasticsVO2 max030229 sport sciencesExercise physiologyIntensity (physics)Antioxidant capacityAntioxidant capacity030104 developmental biologyEndocrinologyOxidative stressPhysical therapyAntioxidant capacity Exercise physiology Oxidative stress Free radicals Reactive oxygen species Training intensity Rhythmic gymnasticsbusinesslcsh:RC1200-1245Reactive oxygen speciesSettore M-EDF/01 - Metodi E Didattiche Delle Attivita' MotorieAnaerobic exerciseOxidative stressJournal of Sport and Health Science
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Iron-loaded transferrin (Tf) is detrimental whereas iron-free Tf confers protection against brain ischemia by modifying blood Tf saturation and subse…

2018

Despite transferrin being the main circulating carrier of iron in body fluids, and iron overload conditions being known to worsen stroke outcome through reactive oxygen species (ROS)-induced damage, the contribution of blood transferrin saturation (TSAT) to stroke brain damage is unknown. The objective of this study was to obtain evidence on whether TSAT determines the impact of experimental ischemic stroke on brain damage and whether iron-free transferrin (apotransferrin, ATf)-induced reduction of TSAT is neuroprotective. We found that experimental ischemic stroke promoted an early extravasation of circulating iron-loaded transferrin (holotransferrin, HTf) to the ischemic brain parenchyma.…

0301 basic medicineU-PAGE urea-polyacrylamide gel electrophoresisMaleClinical BiochemistryExperimental strokeBiochemistryBrain IschemiaBrain ischemia0302 clinical medicineADC apparent diffusion coefficientApotransferrinDWI diffusion-weighted imagingTANDEM-1 Thrombolysis and Deferoxamine in Middle Cerebral Artery Occlusion clinical trialrHTf rat HTfrATf rat ATflcsh:QH301-705.5chemistry.chemical_classificationNeuronslcsh:R5-920ChemistryTransferrinExtravasationNS21 a medium supplement to grow neuronspDAPK-1 phosphorylated anti-death-associated protein kinase 1NeuroprotectionStrokeWB Western blotFemalemedicine.symptomlcsh:Medicine (General)Research PaperhHTf human HTfPC12 cell line derived from a pheochromocytoma of the rat adrenal medullamedicine.medical_specialtyIron OverloadBBB blood-brain barrierNMDAR N-methyl-D-aspartate receptorDCF dihydrofluoresceinIronWGA wheat germ agglutininHTf holotransferrinTransferrin receptorBrain damageTfR transferrin receptorDeferoxamineNeuroprotectionPI propidium iodide03 medical and health sciencesBrain damageCM conditioned mediumROS reactive oxygen speciesInternal medicine4-HNE 4-hydroxynonenalTf transferrinReceptors TransferrinmedicineFeRhoNoxTM-1 probe to detect Fe2+AnimalsHumansATf apotransferrinCM-H2DCFDA 5-chloromethyl-27-dichlorodihydrofluorescein diacetateMCAO middle cerebral artery occlusionDMT-1 divalent metal transporterB-27 a medium supplement to grow neuronsReactive oxygen speciesNMDA N-methyl-D-aspartateTSAT blood transferrin saturationTransferrin saturationBlood transferrin saturation (TSAT)Organic ChemistryNIR near infraredReactive oxygen species (ROS)medicine.diseasepMCAO permanent middle cerebral artery occlusionRatsPWI perfusion-weighted imaging030104 developmental biologyEndocrinologylcsh:Biology (General)TransferrinDAPK-1 anti-death-associated protein kinaseOGD oxygen/glucose deprivationTTC 235-triphenyl-tetrazolium chlorideLipid PeroxidationMCA middle cerebral arteryApoproteinsReactive Oxygen SpeciesMRI magnetic resonance imagingtMCAO transient middle cerebral artery occlusion030217 neurology & neurosurgeryhATf human ATf
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Validación del cribado nutricional Malnutrition Screening Tool comparado con la valoración nutricional completa y otros cribados en distintos ámbitos…

2018

Resumen Introducción: los cribados son empleados para detectar el riesgo nutricional y permiten intervenir precozmente e influir en el pronóstico. La herramienta Malnutrition Screening Tool (MST) está validada en paciente hospitalizado y oncológico ambulatorio. Objetivo: analizar por ámbitos sociosanitarios y grupos etarios los cribados MST, Malnutrition Universal Screening Tool (MUST) y Mini Nutritional Assessment-Short Form (MNA-sf) respecto a la valoración nutricional completa (VNC) como patrón de referencia, y evaluar la utilidad del MST en aquellos ámbitos donde no está validado. Material y métodos: se han incluido 815 sujetos ambulatorios, hospitalizados e institucionalizados. Se les …

0301 basic medicineValidation study030109 nutrition & dieteticsNutrition and DieteticsNutrition assessmentValoración nutricionalbusiness.industryMedicine (miscellaneous)Fiabilidad03 medical and health sciencesMedicineValidezbusinessRiesgo nutricionalCribado nutricionalHumanitiesDesnutrición establecida
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Multicenter validation study for the certification of a CFTR gene scanning method using next generation sequencing technology.

2018

AbstractBackground:Many European laboratories offer molecular genetic analysis of theCFTRgene using a wide range of methods to identify mutations causative of cystic fibrosis (CF) and CFTR-related disorders (CFTR-RDs). Next-generation sequencing (NGS) strategies are widely used in diagnostic practice, and CE marking is now required for most in vitro diagnostic (IVD) tests in Europe. The aim of this multicenter study, which involved three European laboratories specialized in CF molecular analysis, was to evaluate the performance of Multiplicom’s CFTR MASTR Dx kit to obtain CE-IVD certification.Methods:A total of 164 samples, previously analyzed with well-established “reference” methods for t…

0301 basic medicineValidation studycongenital hereditary and neonatal diseases and abnormalitiesCertification[SDV]Life Sciences [q-bio]Clinical BiochemistrySequencing dataCFTR molecular diagnosiCystic Fibrosis Transmembrane Conductance RegulatorComputational biology030105 genetics & heredityBiologyCFTR molecular diagnosisDNA sequencingIn vitro diagnosticCftr genecystic fibrosis03 medical and health sciencesHumanscystic fibrosiCE-IVD certificationBiochemistry (medical)Reproducibility of ResultsIllumina miseqSequence Analysis DNAGeneral MedicineMolecular analysisEurope030104 developmental biologyMulticenter studycomparative sequencing analysicomparative sequencing analysisMutationnext-generation sequencingMultiplex Polymerase Chain Reaction
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The protective value of a defensive display varies with the experience of wild predators

2019

AbstractPredation has driven the evolution of diverse adaptations for defence among prey, and one striking example is the deimatic display. While such displays can resemble, or indeed co-occur with, aposematic ‘warning’ signals, theory suggests deimatic displays may function independently of predator learning. The survival value of deimatic displays against wild predators has not been tested before. Here we used the mountain katydid Acripeza reticulata to test the efficacy of a putative deimatic display in the wild. Mountain katydids have a complex defence strategy; they are camouflaged at rest, but reveal a striking red-, blue-, and black-banded abdomen when attacked. We presented live kat…

0301 basic medicineValue (ethics)Allopatric speciationZoologylcsh:MedicineAposematismeläinten käyttäytyminenArticlePredationGryllidae03 medical and health sciences0302 clinical medicineAnimalspuolustusmekanismit (biologia)Australian magpielcsh:SciencePredatorMultidisciplinaryBehavior Animaldefensive displaybiologylcsh:RAustraliabehavioural ecologyhepokatitbiology.organism_classificationkatydids030104 developmental biologySympatric speciationPredatory BehaviorPredator attacklcsh:Q030217 neurology & neurosurgeryScientific Reports
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Multigram Scale Enzymatic Synthesis of (R)-1-(4′-Hydroxyphenyl)ethanol Using Vanillyl Alcohol Oxidase

2018

The enantioselective oxyfunctionalisation of C−H bonds is a highly interesting reaction, as it provides access to chiral alcohols that are important pharmaceutical building blocks. However, it is hard to achieve using traditional methods. One way in which it can be achieved is through the action of oxidative enzymes. Although many reports of the oxyfunctionalisation capabilities of enzymes at an analytical scale have been published, reports on the use of enzymes to achieve oxyfunctionalisation on a synthetically relevant scale are fewer. Here, we describe the scale-up of the conversion of 4-ethylphenol to (R)-1-(4′-hydroxyphenyl)ethanol using the flavin-dependent enzyme vanillyl alcohol oxi…

0301 basic medicineVanillyl-alcohol oxidaseBiochemieEnantioselectivityHydroxylation01 natural sciencesBiochemistryHydroxylation03 medical and health scienceschemistry.chemical_compoundOxidative enzymeOrganic chemistryVLAGchemistry.chemical_classificationEthanol010405 organic chemistryChemistryEnantioselective synthesisSubstrate (chemistry)General ChemistryAlkylphenols0104 chemical sciencesFlavoprotein030104 developmental biologyEnzymeYield (chemistry)AlcoholsOxidoreductases
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Oxidative stress in retinal pigment epithelium cells increases exosome secretion and promotes angiogenesis in endothelial cells.

2015

10 páginas, 5 figuras

0301 basic medicineVascular Endothelial Growth Factor AAngiogenesisretinal pigment epitheliumNeovascularization PhysiologicexosomesBiologyExosomesExosomeCell Line03 medical and health sciencesangiogenesismedicineHuman Umbilical Vein Endothelial CellsHumansRNA MessengerRetinal pigment epitheliumVEGF receptorsTube formationRetinal pigment epitheliumEthanolCell BiologyOriginal ArticlesMicrovesicleseye diseasesCell biologyEndothelial stem cellVascular endothelial growth factor AOxidative Stress030104 developmental biologymedicine.anatomical_structureReceptors Vascular Endothelial Growth FactorOxidative stressCell cultureMolecular MedicineOriginal ArticleAngiogenesissense organsJournal of cellular and molecular medicine
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The Relationship between Diet and Frailty in Aging

2019

The increase in lifespan in the 20th century entails an increase in the elderly population. This brings a new challenge for society, causing people to have physical and mental limitations caused by age-related diseases, such as frailty. Frailty is clinically characterized by multisystem pathophysiological processes, such as chronic inflammation, immune activation, dysregulation of the musculoskeletal and endocrine systems, oxidative stress, energy imbalances, mitochondrial dysfunction, and sarcopenia. The elderly should consume energy in amounts close to those in what is currently accepted as a balanced diet. However, an increase in protein intake may be recommended for elderly people as l…

0301 basic medicineVitaminGerontologyAgingFrail ElderlyEndocrinology Diabetes and MetabolismNutritional Status030209 endocrinology & metabolismInflammationmedicine.disease_causeEating03 medical and health scienceschemistry.chemical_compound0302 clinical medicineNutraceuticalmedicineAnimalsHumansImmunology and AllergyEndocrine systemAgedAged 80 and overFrailtybusiness.industrymedicine.diseaseComorbidityDiet030104 developmental biologyHuman nutritionchemistrySarcopeniaDiet Healthymedicine.symptomEnergy IntakebusinessOxidative stressEndocrine, Metabolic & Immune Disorders - Drug Targets
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Vitamin D and Cardiovascular Health

2021

The principal source of vitamin D in humans is its biosynthesis in the skin through a chemical reaction dependent on sun exposure. In lesser amounts, the vitamin can be obtained from the diet, mostly from fatty fish, fish liver oil and mushrooms. Individuals with vitamin D deficiency, defined as a serum level of 25 hydroxyvitamin D < 20 ng/dl, should be supplemented. Vitamin D deficiency is a prevalent global problem caused mainly by low exposure to sunlight. The main role of 1,25 dihydroxyvitamin D is the maintenance of calcium and phosphorus homeostasis. However, vitamin D receptors are found in most human cells and tissues, indicating many extra-skeletal effects of the vitamin, particula…

0301 basic medicineVitaminmedicine.medical_specialtyVitamina DEnfermedad cardiovascularchemistry.chemical_element030209 endocrinology & metabolismCalciumCritical Care and Intensive Care Medicinemedicine.disease_causevitamin D deficiencyNitric oxide03 medical and health scienceschemistry.chemical_compound0302 clinical medicineImmune systemInternal medicineHipertensiónmedicineVitamin D and neurologyHumansSupplementsVitamin D030109 nutrition & dieteticsNutrition and DieteticsEnfermedad coronariabusiness.industryEndothelial functionVitamin D Deficiencymedicine.diseaseCardiovascular diseaseCoronary heart diseaseEndocrinologyDietéticachemistryCardiovascular DiseasesNutriciónHypertensionNarrative ReviewbusinessHomeostasisOxidative stress
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Viable But Not Culturable (VBNC) state of Brettanomyces bruxellensis in wine: New insights on molecular basis of VBNC behaviour using a transcriptomi…

2016

International audience; The spoilage potential of Brettanomyces bruxellensis in wine is strongly connected with the aptitude of this yeast to enter in a Viable But Non Culturable (VBNC) state when exposed to the harsh wine conditions. In this work, we characterized the VBNC behaviour of seven strains of B. bruxellensis representing a regional intraspecific biodiversity, reporting conclusive evidence for the assessment of VBNC as a strain-dependent character. The VBNC behaviour was monitored by fluorescein diacetate staining/flow cytometry for eleven days after addition of 0.4, 0.6, 0.8, 1 and 1.2 mg/L of molecular SO2 (entrance in the VBNC state) and after SO2 removal (exit from the VBNC st…

0301 basic medicine[ SDV.AEN ] Life Sciences [q-bio]/Food and NutritionColony Count MicrobialExpressionSaccharomyces-cerevisiaeTranscriptometranscriptomicsHomeostasisSulfur DioxideHeat-Shock Proteinsmedicine.diagnostic_testViabilityCarbohydrate MetabolismOxidation-ReductionVolatile phenol production030106 microbiologyBrettanomyces bruxellensisBrettanomycesBiologyFlow cytometryMicrobiology03 medical and health sciencesPhenolsHeat shock proteinsulphitemedicineSulfiteswineGeneRna-seqBrettanomyces; spoilage; sulphite; transcriptomics; Viable But Not Culturable (VBNC); wine; food science; microbiologyWineMicrobial ViabilityGene Expression ProfilingspoilagemicrobiologyDNA replicationNonculturable bacteriabiology.organism_classificationCampylobacter-jejuniSulfur-dioxideYeastYeastCulture MediaOxidative StressFood MicrobiologyViable But Not Culturable (VBNC)food science[SDV.AEN]Life Sciences [q-bio]/Food and NutritionSettore AGR/16 - Microbiologia Agraria
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