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showing 10 items of 3080 documents

Gray Matter NG2 Cells Display Multiple Ca2+-Signaling Pathways and Highly Motile Processes

2011

NG2 cells, the fourth type of glia in the mammalian CNS, receive synaptic input from neurons. The function of this innervation is unknown yet. Postsynaptic changes in intracellular Ca(2+)-concentration ([Ca(2+)](i)) might be a possible consequence. We employed transgenic mice with fluorescently labeled NG2 cells to address this issue. To identify Ca(2+)-signaling pathways we combined patch-clamp recordings, Ca(2+)-imaging, mRNA-transcript analysis and focal pressure-application of various substances to identified NG2-cells in acute hippocampal slices. We show that activation of voltage-gated Ca(2+)-channels, Ca(2+)-permeable AMPA-receptors, and group I metabotropic glutamate-receptors provo…

Central Nervous SystemAnatomy and PhysiologyVesicular glutamate transporter 1Glycobiologylcsh:MedicineHippocampal formationBiochemistryIon ChannelsTransmembrane Transport ProteinsMice0302 clinical medicinePostsynaptic potentialBiomacromolecule-Ligand Interactionslcsh:ScienceCells CulturedMembrane potential0303 health sciencesMultidisciplinarybiologyReverse Transcriptase Polymerase Chain ReactionDepolarizationNeurochemistryNeurotransmittersCell biologyElectrophysiologymedicine.anatomical_structureNeurologyNeurogliaMedicineProteoglycansNeurochemicalsGlutamateNeurogliaResearch ArticleNervous System PhysiologySignal TransductionCell PhysiologyMotilityNeuroimagingMice TransgenicNeurological System03 medical and health sciencesNeuropharmacologymedicineAnimalsHumansddc:610Biology030304 developmental biologyEndoplasmic reticulumlcsh:RProteinsGamma-Aminobutyric AcidTransmembrane ProteinsLuminescent ProteinsMicroscopy Electronnervous systemMicroscopy FluorescenceSynapsesVesicular Glutamate Transport Protein 1biology.proteinNervous System Componentslcsh:QCalciumPhysiological Processes030217 neurology & neurosurgeryNeurosciencePLoS ONE
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IkappaB kinase 2 determines oligodendrocyte loss by non-cell-autonomous activation of NF-kappaB in the central nervous system

2011

The IκB kinase complex induces nuclear factor kappa B activation and has recently been recognized as a key player of autoimmunity in the central nervous system. Notably, IκB kinase/nuclear factor kappa B signalling regulates peripheral myelin formation by Schwann cells, however, its role in myelin formation in the central nervous system during health and disease is largely unknown. Surprisingly, we found that brain-specific IκB kinase 2 expression is dispensable for proper myelin assembly and repair in the central nervous system, but instead plays a fundamental role for the loss of myelin in the cuprizone model. During toxic demyelination, inhibition of nuclear factor kappa B activation by …

Central Nervous SystemBlotting WesternIκB kinaseBiologyddc:616.07Myelin assemblyMicroglia/cytology/metabolismNerve Regeneration/physiologyDemyelinating Diseases/chemically induced/metabolism03 medical and health sciencesMyelinCuprizoneMice0302 clinical medicineCentral Nervous System/cytology/metabolismmedicineAnimalsRemyelinationCHUKMyelin Sheath030304 developmental biologyAstrocytes/cytology/metabolismMyelin Sheath/metabolism0303 health sciencesReverse Transcriptase Polymerase Chain ReactionSignal Transduction/physiologyI-Kappa-B KinaseNF-kappa BI-kappa B Kinase/metabolismOriginal ArticlesOligodendrocyte3. Good healthCell biologyI-kappa B KinaseNerve RegenerationOligodendrogliamedicine.anatomical_structureOligodendroglia/metabolismAstrocytesNF-kappa B/metabolismNeurogliaNeurology (clinical)MicrogliaNeuroscience030217 neurology & neurosurgeryDemyelinating DiseasesSignal Transduction
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The Relationship between Gray Matter Quantitative MRI and Disability in Secondary Progressive Multiple Sclerosis

2016

Purpose: In secondary progressive Multiple Sclerosis (SPMS), global neurodegeneration as a driver of disability gains importance in comparison to focal inflammatory processes. However, clinical MRI does not visualize changes of tissue composition outside MS lesions. This quantitative MRI (qMRI) study investigated cortical and deep gray matter (GM) proton density (PD) values and T1 relaxation times to explore their potential to assess neuronal damage and its relationship to clinical disability in SPMS. Materials and Methods: 11 SPMS patients underwent quantitative T1 and PD mapping. Parameter values across the cerebral cortex and deep GM structures were compared with 11 healthy controls, and…

Central Nervous SystemMalePathologyPhysiologylcsh:MedicinePathology and Laboratory MedicineNervous SystemBrain mappingDiagnostic Radiology030218 nuclear medicine & medical imaging0302 clinical medicineThalamusMedicine and Health SciencesRelaxation TimeMedicineGray Matterlcsh:ScienceCerebrospinal FluidCerebral CortexMultidisciplinarymedicine.diagnostic_testRadiology and ImagingPhysicsPutamenNeurodegenerationBrainNeurodegenerative DiseasesMultiple Sclerosis Chronic ProgressiveMagnetic Resonance ImagingBody Fluidsmedicine.anatomical_structureNeurologyCerebral cortexPhysical SciencesFemaleAnatomyResearch ArticleAdultmedicine.medical_specialtyMultiple SclerosisImaging TechniquesImmunologyCentral nervous systemThalamusResearch and Analysis MethodsAutoimmune Diseases03 medical and health sciencesSigns and SymptomsDiagnostic MedicineIntellectual DisabilityHumansddc:610Relaxation (Physics)business.industryMultiple sclerosislcsh:RBiology and Life SciencesMagnetic resonance imagingmedicine.diseaseDemyelinating DisordersCase-Control StudiesLesionslcsh:QClinical ImmunologyClinical Medicinebusiness030217 neurology & neurosurgeryPLOS ONE
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The angiopoietin-Tie2 pathway regulates Purkinje cell dendritic morphogenesis in a cell-autonomous manner.

2021

Neuro-vascular communication is essential to synchronize central nervous system development. Here, we identify angiopoietin/Tie2 as a neuro-vascular signaling axis involved in regulating dendritic morphogenesis of Purkinje cells (PCs). We show that in the developing cerebellum Tie2 expression is not restricted to blood vessels, but it is also present in PCs. Its ligands angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2) are expressed in neural cells and endothelial cells (ECs), respectively. PC-specific deletion of Tie2 results in reduced dendritic arborization, which is recapitulated in neural-specific Ang1-knockout and Ang2 full-knockout mice. Mechanistically, RNA sequencing reveals that Tie…

CerebellumalphaCytoskeleton organizationAngiogenesisPurkinje cellprotocadherinsMorphogenesisneural progenitor cellsMice Transgenicself-avoidanceBiologyModels BiologicalGeneral Biochemistry Genetics and Molecular BiologyAngiopoietinAngiopoietin-2Purkinje Cellsddc:570CerebellumexpressionGene expressionmedicineAngiopoietin-1MorphogenesisAnimalsmouseMice KnockoutIntegrasessubventricular zonedifferentiationDendritesmtorc2Angiopoietin receptorReceptor TIE-2Cell biologyMice Inbred C57BLmedicine.anatomical_structuremessenger-rnaGene Expression RegulationOrgan Specificityembryonic structurescardiovascular systembiology.proteinGene DeletionSignal TransductionCell reports
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Exchange rates expectations and chaotic dynamics: a replication study

2018

Abstract In this paper the author analyzes the behavior of exchange rates expectations for four currencies, by considering a re-calculation and an extension of Resende and Zeidan (Expectations and chaotic dynamics: empirical evidence on exchange rates, Economics Letters, 2008). Considering Lyapunov exponent-based tests results, they are not supportive of chaos in exchange rates expectations, although the so-called 0–1 test strongly supports the chaos hypothesis.

ChaoticSocial SciencesLyapunov exponent01 natural sciencesexchange rates010305 fluids & plasmassymbols.namesakeH0502 economics and business0103 physical sciencesReplication (statistics)ddc:330Statistical physicsC15050207 economicsEmpirical evidenceHB71-74MathematicsC120-1 testdeterministic chaos05 social sciencesDynamics (mechanics)Lyapunov exponentsNonlinear Sciences::Chaotic DynamicsEconomics as a sciencesymbolsGeneral Economics Econometrics and Financeexpectations
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On the use of fractional calculus for the probabilistic characterization of random variables

2009

In this paper, the classical problem of the probabilistic characterization of a random variable is re-examined. A random variable is usually described by the probability density function (PDF) or by its Fourier transform, namely the characteristic function (CF). The CF can be further expressed by a Taylor series involving the moments of the random variable. However, in some circumstances, the moments do not exist and the Taylor expansion of the CF is useless. This happens for example in the case of $\alpha$--stable random variables. Here, the problem of representing the CF or the PDF of random variables (r.vs) is examined by introducing fractional calculus. Two very remarkable results are o…

Characteristic function (probability theory)FOS: Physical sciencesAerospace EngineeringMathematics - Statistics TheoryOcean EngineeringProbability density functionComplex order momentStatistics Theory (math.ST)Fractional calculusymbols.namesakeIngenieurwissenschaftenFOS: MathematicsTaylor seriesApplied mathematicsCharacteristic function serieMathematical PhysicsCivil and Structural EngineeringMathematicsGeneralized Taylor serieMechanical EngineeringStatistical and Nonlinear PhysicsProbability and statisticsMathematical Physics (math-ph)Condensed Matter PhysicsFractional calculusFourier transformNuclear Energy and EngineeringPhysics - Data Analysis Statistics and ProbabilitysymbolsFractional calculus; Generalized Taylor series; Complex order moments; Fractional moments; Characteristic function series; Probability density function seriesddc:620Series expansionFractional momentProbability density function seriesSettore ICAR/08 - Scienza Delle CostruzioniRandom variableData Analysis Statistics and Probability (physics.data-an)
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Immunotherapy With Human Gamma Delta T Cells—Synergistic Potential of Epigenetic Drugs?

2018

Epigenetics has emerged as one of the fastest growing concepts, adding more than 45 new publications every day, spreading through various fields ( 1). Conrad Waddington coined the term “epigenetics” in 1942; however, a multitude of definitions has been endorsed by different researchers. In essence, Waddington’s definition of “epigenetics” and its redefinition by Holiday is at the heart of cellular function. Hence, it is obvious that epigenetic regulation plays a central role also in the specification, differentiation, and functional plasticity of T lymphocytes ( 2). T-cell fate decision in progenitor cells, functional CD4 T-cell plasticity, CD8 T-cell differentiation, but also T-cell memory…

Checkpoint Inhibitorslcsh:Immunologic diseases. Allergy0301 basic medicineDeltaOpinionmedicine.medical_treatmentImmune checkpoint inhibitorsCell PlasticityImmunologyNatural-killer Group 2 Member DBiologyEpigenesis Genetic03 medical and health sciencesCell Plasticitymedicineddc:6AnimalsHumansgamma delta T cellsImmunology and Allergyddc:610EpigeneticsIntraepithelial Lymphocytesprogrammed death 1DNA methylationnatural-killer group 2 member DProgrammed Death 1articlehistone acetylationGamma Delta T CellsImmunotherapy030104 developmental biologyHistone acetylationDNA methylationCancer researchIntraepithelial lymphocyteBromodomain And Extraterminal DomainBromodomain and ExtraTerminal domainImmunotherapyimmunotherapyProgrammed death 1lcsh:RC581-607checkpoint inhibitorsFrontiers in Immunology
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Plasmonic Resonant Nanoantennas Induce Changes in the Shape and the Intensity of Infrared Spectra of Phospholipids.

2021

Surface enhanced infrared absorption spectroscopic studies (SEIRAS) as a technique to study biological molecules in extremely low concentrations is greatly evolving. In order to use the technique for identification of the structure and interactions of such biological molecules, it is necessary to identify the effects of the plasmonic electric-field enhancement on the spectral signature. In this study the spectral properties of 1,2-Dipalmitoyl-sn-glycero-3 phosphothioethanol (DPPTE) phospholipid immobilized on gold nanoantennas, specifically designed to enhance the vibrational fingerprints of lipid molecules were studied. An AFM study demonstrates an organization of the DPPTE phospholipid in…

Chemical PhenomenaSpectrophotometry InfraredLipid BilayersPharmaceutical ScienceMetal NanoparticleslipiditMicroscopy Atomic ForcebiomolekyylitkultaArticleAnalytical ChemistryQD241-441nanorakenteetDrug Discoveryddc:530Physical and Theoretical ChemistryDPPTEenhancementPhospholipidsSEIRASnanoantennas; DPPTE; bilayers; SEIRAS; enhancement; AFMPhysicsOrganic ChemistryTemperatureinfrapunaspektroskopiaSurface Plasmon ResonanceNanostructuresnanoantennasChemistry (miscellaneous)Molecular MedicineGoldAFMbilayersMolecules (Basel, Switzerland)
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Setting the photoelectron clock through molecular alignment

2020

The interaction of strong laser fields with matter intrinsically provides a powerful tool for imaging transient dynamics with an extremely high spatiotemporal resolution. Here, we study strong-field ionisation of laser-aligned molecules, and show a full real-time picture of the photoelectron dynamics in the combined action of the laser field and the molecular interaction. We demonstrate that the molecule has a dramatic impact on the overall strong-field dynamics: it sets the clock for the emission of electrons with a given rescattering kinetic energy. This result represents a benchmark for the seminal statements of molecular-frame strong-field physics and has strong impact on the interpreta…

Chemical Physics (physics.chem-ph)Atomic Physics (physics.atom-ph)ScienceChemical physicsQQuantum physicsFOS: Physical sciencesSettore FIS/03 - Fisica Della MateriaArticlePhysics - Atomic PhysicsStrong field ionizationTDDFTPhysics - Chemical Physicslcsh:QAtomic and molecular physicsddc:500Physics - Atomic and Molecular Clusterslcsh:ScienceAtomic and Molecular Clusters (physics.atm-clus)Applied optics
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Photochemically induced dynamic nuclear polarization of heteronuclear singlet order

2021

Photochemically induced dynamic nuclear polarization (photo-CIDNP) is a method to hyperpolarize nuclear spins using light. In most cases, CIDNP experiments are performed in high magnetic fields and the sample is irradiated by light inside a nuclear magnetic resonance (NMR) spectrometer. Here we demonstrate photo-CIDNP hyperpolarization generated in the Earth's magnetic field and under zero- to ultralow-field (ZULF) conditions. Irradiating a sample containing tetraphenylporphyrin and para-benzoquinone for several seconds with light-emitting diodes produces strong hyperpolarization of 1H and 13C nuclear spins, enhancing the NMR signals more than 200 times. The hyperpolarized spin states at th…

Chemical Physics (physics.chem-ph)Materials scienceSpin statesSpinsField (physics)CIDNPPhysics::Medical PhysicsFOS: Physical sciences02 engineering and technology010402 general chemistry021001 nanoscience & nanotechnologyPolarization (waves)7. Clean energy01 natural sciences0104 chemical sciencesMagnetic fieldHeteronuclear moleculePhysics - Chemical Physicsddc:530General Materials ScienceSinglet statePhysical and Theoretical ChemistryAtomic physics0210 nano-technology
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