Search results for "death"
showing 10 items of 1744 documents
Apigenin affects leptin/leptin receptor pathway and induces cell apoptosis in lung adenocarcinoma cell line
2011
Abstract Background Apigenin, a common edible plant flavonoid, is a well characterised antioxidant. The adipokine leptin exerts proliferative and anti-apoptotic activities in a variety of cell types. In cancer cells, apigenin may induce a pro-apoptotic pathway whereas leptin has an anti-apoptotic role. The purpose of the study is to investigate the role of apigenin and of leptin/leptin receptor pathway on proliferation and on apoptosis in lung adenocarcinoma. Methods Immunocytochemistry, flow cytometry and RT-q-RT PCR, were used to investigate the expression and modulation of leptin receptors on the lung adenocarcinoma cell line A549 in presence or absence of apigenin and of leptin, alone o…
Ripensare lo straniero. Lesbii e Parti nell'ottavo libro del Bellum civile di Lucano
2016
The eighth book of Lucan's bellum ciuile opens with the loss of Pompeius fleeing from the Thessalian battlefield. This departure, which sees the old fighter now prostrate and on which hangs a heavy fate of death, includes a double description of peoples and nations. The inhabitants of Lesbos, in which Cornelia had found shelter, offer a safe haven to Pompeius, urging him to remain. Moreover Pompeius seem to be inclined to the Parthians, the traditional enemy of Rome, but the option is destined to be defeated at the urging of Lentulus, which will convince the council of war to turn to Egypt. Within this opposing and contradictory motivations, where the reasons of the history emerge strongly …
NFATc1 Induction in Peripheral T and B Lymphocytes
2013
Abstract NFAT transcription factors control the proliferation and survival of peripheral lymphocytes. We have reported previously that the short isoform NFATc1/αA whose generation is induced by immune receptor stimulation supports the proliferation and inhibits the activation-induced cell death of peripheral T and B cells. We will show in this study that in novel bacterial artificial chromosome transgenic mice that express EGFP under the control of entire Nfatc1 locus the Nfatc1/Egfp transgene is expressed as early as in double-negative thymocytes and in nonstimulated peripheral T and B cells. Upon immune receptor stimulation, Nfatc1/Egfp expression is elevated in B, Th1, and Th2 cells, but…
LPS-induced microglial secretion of TNFα increases activity-dependent neuronal apoptosis in the neonatal cerebral cortex.
2012
During the pre- and neonatal period, the cerebral cortex reveals distinct patterns of spontaneous synchronized activity, which is critically involved in the formation of early networks and in the regulation of neuronal survival and programmed cell death (apoptosis). During this period, the cortex is also highly vulnerable to inflammation and in humans prenatal infection may have a profound impact on neurodevelopment causing long-term neurological deficits. Using in vitro and in vivo multi-electrode array recordings and quantification of caspase-3 (casp-3)-dependent apoptosis, we demonstrate that lipopolysaccharide-induced inflammation causes rapid alterations in the pattern of spontaneous b…
Ablation of c-FLIP in hepatocytes enhances death-receptor mediated apoptosis and toxic liver injury in vivo
2010
Background & Aims Apoptosis is crucially involved in acute and chronic liver injury, including viral, cholestatic, toxic, and metabolic liver disease. Additionally, dysregulation of apoptosis signaling pathways has been implicated in hepatocarcinogenesis. The most prominent members of the apoptosis-mediating tumor necrosis factor receptor superfamily are the TNF-R1 (CD120a) and the CD95 (Apo-1/Fas) receptor. Although extensively studied, the intracellular signaling events in hepatocytes are only incompletely understood. Methods To examine the role of the caspase-8 homolog cellular FLICE-inhibitory protein (c-FLIP) in liver injury, we generated mice with hepatocyte specific deletion of c-FLI…
Bcl-2 is a negative regulator of interleukin-1β secretion in murine macrophages in pharmacological-induced apoptosis
2010
BACKGROUND AND PURPOSE Cucurbitacin R, a natural anti-inflammatory product, has been shown to exhibit activity against both adjuvant-induced arthritis and delayed-type hypersensitivity reactions induced by various agents. Previous studies have demonstrated that the effects of cucurbitacin R stem from its inhibition of both cytokine production and lymphocyte proliferation. EXPERIMENTAL APPROACHES Effects of cucurbitacin R were investigated on lipopolysaccharide-stimulated RAW 264.7 cells. Cell cycle evolution was analysed by flow cytometry, detection of apoptosis by DNA ladder, Bcl-2, p21, p53, Bax, cleaved caspase-1 (p10), caspase-9, and caspase-3, cleaved caspase (p17) and interleukin-1β d…
Dominant negative MORT1/FADD rescues mice from CD95 and TNF-induced liver failure
2002
Derangement of the apoptotic program is considered an important cause of liver disease. It became clear that receptor-mediated apoptosis is of specific interest in this context, and CD95 and CD120a, both members of the tumor necrosis factor (TNF) receptor superfamily, are the most prominent cell death receptors involved. The death signal is induced upon ligand binding by recruitment of caspases via the adapter molecule MORT1/FADD to the receptor and their subsequent activation. To investigate the role of MORT1/FADD in hepatocyte apoptosis, we generated transgenic mice expressing liver-specific dominant negative mutant. Mice looked grossly normal; breeding and liver development were not diff…
El «Poema del otoño», de Rubén Darío, consolación de la poesía
2017
El trabajo insiste en el carácter fundacional de la obra de Darío en la poesía hispana debido, entre otras razones, a su poética especialmente dialogante. Poesía humanamente docta y erudita, el «Poema del otoño» pretende otorgar consuelo ante la idea de la muerte. En un contexto secularizado, Darío busca dar cuerpo a un nuevo absoluto, un arte que venza tiempo y espacio. La consolatio asume el tópico del carpe diem, pues el goce de la carne, como la contemplación de la belleza del mundo, como el placer del canto, no solo son experiencias sensibles, sino también simbólicas, que permiten intuir o recordar el orden íntegro y armónico del universo. This work focuses in the foundational role of …
La ejemplaridad de la muerte y la inmortalidad del saber en la literatura sapiencial medieval
2022
El origen escriturario de la muerte como castigo es el punto de partida de su ejemplaridad que, basada en presupuestos teológicos, le imprimió un halo de penitencia, que se perfila en todos los órdenes de actuación y convivencia del ser humano, en aras de conseguir el perdón y retornar a la gracia divina. En este trabajo se analizan los diferentes motivos que proyectan la ejemplaridad de la muerte en el ámbito de la literatura sapiencial: muerte física y muerte espiritual; trascendencia y sentencia del alma; el hecho maravilloso (milagros, premoniciones, relevaciones o visiones); el miedo (y lo macabro) ligado al arquetipo de la muerte transida y sus plasmaciones en variados tópicos (mement…
Necrológica del <i>Outsider</i> Reinhart Koselleck: el «historiador pensante» y las polémicas de los historiadores
2007
Reinhart Koselleck is an author difficult to classify, since his conceptual history is rejected, and at the same time taken advantage of, by both philosophy and historiography. This paper analyses Koselleck’s attitude, sometimes tactful, at other times passionate, towards the polemics of the historians and philosophers of his country, and particularly towards the controversy concerning the involvement of both groups in Nazism—a controversy that has provoked a debate, still open, about the affections and disaffections between the science which studies the past, and memory, and has also unleashed a dispute about memorials motivated by the monument to the victims of the Holocaust. Koselleck tr…