Search results for "development."

Impact assessment of the soil and water conservation project: Participatory Impact Assessment

2006

A Coding Scheme Development Methodology Using Grounded Theory for Qualitative Analysis of Pair Programming

2008

A number of quantitative studies of pair programming (the practice of two programmers working together using just one computer) have partially conflicting results. Qualitative studies are needed to explain what is really going on. We support such studies by taking a grounded theory (GT) approach for deriving a coding scheme for the objective conceptual description of specific pair programming sessions independent of a particular research goal. The present article explains why our initial attempts at using GT failed and describes how to avoid these difficulties by a predetermined perspective on the data, concept naming rules, an analysis results metamodel, and pair coding. These practices ma…

Mobility of Cultures and Knowledge Management in Contemporary Europe

2012

In the aftermath of the fall of the Iron Curtain, the European Union (EU) has included more and more new member states from Central and Eastern parts of the European continent. This enlargement process has increased the cultural diversity of the European community as new languages and minority groups have been subsumed into the EU. It is the purpose of this article to discuss the challenges that result from the EU's enlargement, together with the added intra-European mobility of cultures, that affect the national knowledge infrastructures. Based on recent social scientific scholarship on mobility and cultures, this article proposes that knowledge management in contemporary Europe is not onl…

L’aire urbaine de Valencia dans le système global des villes : Les effets de la crise économique sur le réseau des liens financiers des entreprises m…

2021

El artículo analiza el impacto que la crisis económica ha tenido sobre las redes de relaciones de propiedad-filiación de las empresas multinacionales, consideradas en la literatura científica como uno de los factores más importantes en la integración de las ciudades en la globalización. El caso de estudio es el área metropolitana de Valencia, un centro de segundo nivel en las redes económicas mundiales que ha sido duramente golpeado por los efectos de la crisis desde 2008. A partir del análisis de bases de datos de empresas multinacionales en dos fechas de referencia, 2010 y 2013, se constata que la crisis económica ha provocado un retroceso en la inserción del área metropolitana en dichas …

Error messages in relational database management systems: A comparison of effectiveness, usefulness, and user confidence

2021

Abstract The database and the database management system (DBMS) are two of the main components of any information system. Structured Query Language (SQL) is the most popular query language for retrieving data from the database, as well as for many other data management tasks. During system development and maintenance, software developers use a considerable amount of time to interpret compiler error messages. The quality of these error messages has been demonstrated to affect software development effectiveness, and correctly formulating queries and fixing them when needed is an important task for many software developers. In this study, we set out to investigate how participants ( N = 152 ) …

CVB3 VP1 interacts with MAT1 to inhibit cell proliferation by interfering with Cdk-activating kinase complex activity in CVB3-induced acute pancreati…

2021

Coxsackievirus B3 (CVB3) belongs to the genus Enterovirus of the family Picornaviridae and can cause acute acinar pancreatitis in adults. However, the molecular mechanisms of pathogenesis underlying CVB3-induced acute pancreatitis have remained unclear. In this study, we discovered that CVB3 capsid protein VP1 inhibited pancreatic cell proliferation and exerted strong cytopathic effects on HPAC cells. Through yeast two-hybrid, co-immunoprecipitation, and confocal microscopy, we show that Menage a trois 1 (MAT1), a subunit of the Cdk-Activating Kinase (CAK) complex involved in cell proliferation and transcription, is a novel interaction protein with CVB3 VP1. Moreover, CVB3 VP1 inhibited MAT…

Internalization of novel non-viral vector TAT-streptavidin into human cells

2007

BMC Biotechnology, 7 (1)

The Nasal Epithelium as a Factory for Systemic Protein Delivery

2002

We have previously shown that recombinant Sendai virus (SeV) produces efficient in vivo airway epithelial gene transfer. The ability to produce therapeutic levels of circulating proteins following noninvasive gene transfer would have widespread clinical application. Here, we compared nose, lung, and skeletal muscle for the ability to produce circulating levels of the secreted mouse antiinflammatory cytokine interleukin-10 (IL10) following SeV-mediated gene transfer. High levels of serum IL10 were obtained from each site with a potency order of lung > nose > muscle for a given viral titer. Serum levels from each site were within the likely required range for anti-inflammatory effects. The co…

The closest relatives of icosahedral viruses of thermophilic bacteria are among viruses and plasmids of the halophilic archaea.

2009

We have sequenced the genome and identified the structural proteins and lipids of the novel membranecontaining, icosahedral virus P23-77 of Thermus thermophilus. P23-77 has an 17-kb circular double-stranded DNA genome, which was annotated to contain 37 putative genes. Virions were subjected to dissociation analysis, and five protein species were shown to associate with the internal viral membrane, while three were constituents of the protein capsid. Analysis of the bacteriophage genome revealed it to be evolutionarily related to another Thermus phage (IN93), archaeal Halobacterium plasmid (pHH205), a genetic element integrated into Haloarcula genome (designated here as IHP for integrated Ha…

Lipid Binding Controls Dimerization of the Coat Protein p24 Transmembrane Helix

2019

Abstract Coat protein (COP) I and COP II complexes are involved in the transport of proteins between the endoplasmic reticulum and the Golgi apparatus in eukaryotic cells. The formation of COP I/II complexes at membrane surfaces is an early step in vesicle formation and is mastered by p24, a type I transmembrane protein. Oligomerization of p24 monomers was suggested to be mediated and/or stabilized via interactions within the transmembrane domain, and the p24 transmembrane helix appears to selectively bind a single sphingomyelin C18:0 molecule. Furthermore, a potential cholesterol-binding sequence has also been predicted in the p24 transmembrane domain. Thus, sphingomyelin and/or cholestero…